This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
chemical
product name :
SW48 Cells BAX/BAK (-/-,-/-)
catalog :
CLLS1011
product information
name :
SW48 Cells BAX/BAK (-/-,-/-)
cat no :
CLLS1011
brand :
SIGMA
prod type :
Chemical
storage temp :
-196C
storage :
-196C
general description :
SW48 Cells BAX/BAK (-/-,-/-) are colorectal adenocarcinoma, epithelial cells, from a human caucasian female (aged 82 years), with a ZFN double knock out modification.; This product corresponds to ATCC Cat. No. CCL-231
features and benefits :
Zinc finger nuclease (ZFN) double knock out on chromosome 19q13.3-13.4 / 6p21.3; These SW48 cells are adherent, with a doubling time of approx. 35 hours.
application :
Media Renewal changes one to two times per week; Subculture Ratio: approx. 1:2 to 1:6; Rapidly thaw vial by gentle agitation in 37C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37C in a 5% CO2 atmosphere.; The base medium for this cell line is DMEM, Cat. No. D5671. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10%, Sodium pyruvate, Cat. No. S8636, at a final concentration of 1 mM, and L-glutamine, Cat. No. G7513, at a final concentration of 2mM.; The cell freezing medium is DMSO 1X (Cat. No. C6164)
company information
MilliporeSigma
questions and comments