This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
chemical
product name :
DLD1 Cells HIF1A -/-
catalog :
CLLS1002
product information
name :
DLD1 Cells HIF1A -/-
cat no :
CLLS1002
brand :
SIGMA
prod type :
Chemical
storage temp :
-196C
storage :
-196C
general description :
This product corresponds to ATCC Cat. No.CCL-221; DLD1 Cells HIF1A -/- are colorectal adenocarcinoma, epithelial cells, from a human adult male, with a ZFN knock out modification.
features and benefits :
Zinc finger nuclease (ZFN) knock out on chromosome 14q23.2; These DLD-1 cells are adherent, with a doubling time of approx. 20 hours.
application :
Media Renewal changes two to three times per week; Rapidly thaw vial by gentle agitation in 37C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37C in a 5% CO2 atmosphere.; The cell freezing medium is DMSO 1X (Cat. No. C6164); Subculture Ratio: approx. 1:3 to 1:10; The base medium for this cell line is RPMI, Cat. No. R5886. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10%.
company information
MilliporeSigma
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