A549 Cells GFP-STAT1 | MilliporeSigma CLL1158 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MilliporeSigma

other brands :

FLUKA, Sigma-Aldrich, Roche Applied Science

product type :

chemical

product name :

A549 Cells GFP-STAT1

catalog :

CLL1158

product information

name :

A549 Cells GFP-STAT1

cat no :

CLL1158

brand :

SIGMA

prod type :

Chemical

storage temp :

-196C

storage :

-196C

legal information :

CompoZr is a registered trademark of Sigma-Aldrich Co. LLC

general description :

A549 GFP-STAT1 are lung carcinoma cells from a human 58 year old caucasian male having two distinct ZFN modifications creating two fluorescent transgenes each expressed from their endogenous gene locus.This cell line was derived from ATCC Catalog No. CCL-185.

features and benefits :

Zinc Finger Nuclease (ZFN)-mediated targeted integration of a fluorescent GFP tag into the last coding exon just preceding the stop codon of the STAT1 gene on chromosome 2q32.2 to create a cell line exhibiting stable expression of the GFP transgene.The A549 cells are adherent, with a doubling time of approx. 22 hours.

application :

To learn more, please visit the Cellular Reporter Cell Line webpage; This product is a human A549 cell line in which the genomic STAT1 gene has been endogenously tagged with a Green Fluorescent Protein (GFP) gene using CompoZr(R) zinc finger nuclease (ZFN) technology. Integration resulted in endogenous expression of the fusion protein in which GFP is attached to the C-terminus of STAT1. Fluorescence imaging shows STAT1-GFP expression throughout the cell. Upon activation with a ligand such as interferon-gamma (INF-gamma), the cell line shows redistribution of STAT1 from the cytoplasm to the nucleus, making it useful for high content screening of compounds that modulate STAT1 activity. This stable cell line was expanded from a single clone. The target's gene regulation and corresponding protein function are preserved in contrast to cell lines with overexpression via an exogenous promoter.