U2OS GFP-TUBA1B | MilliporeSigma CLL1031 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MilliporeSigma

other brands :

FLUKA, Sigma-Aldrich, Roche Applied Science

product type :

chemical

product name :

U2OS GFP-TUBA1B

catalog :

CLL1031

product information

name :

U2OS GFP-TUBA1B

cat no :

CLL1031

brand :

SIGMA

prod type :

Chemical

storage temp :

-196C

storage :

-196C

legal information :

CompoZr is a registered trademark of Sigma-Aldrich Co. LLC

general description :

This product corresponds to ATCC Cat. No. HTB-96.; U2OS GFP-TUBA1B are osteosarcoma, epithelial cells, from a human caucasian female (aged 15 years), with a GFP-tagged modification.

features and benefits :

GFP-tagged on chromosome 12q13.12; The U2OS cells are adherent, with a doubling time of approx. 29 hours.

application :

This product is a human U2OS cell line in which the genomic TUBA1B gene has been endogenously tagged with a Green Fluorescent Protein (GFP) gene using CompoZr(R) Zinc Finger Nuclease technology. Integration resulted in endogenous expression of the fusion protein in which GFP is attached to the N-terminus of tubulin. Fluorescence imaging shows that the tubulin can polymerize to form the characteristic pattern of microtubules. Addition of vincristine to this cell line shows the expected depolymerization of microtubules. This stable cell line was expanded from a single clone. The target's gene regulation and corresponding protein function are preserved in contrast to cell lines with overexpression via an exogenous promoter.; To learn more, please visit the Cellular Reporter Cell Line webpage