product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
antibody
product name :
Monoclonal Anti-Collagen, Type X antibody produced in mouse
catalog :
C7974
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
COL-10
reactivity :
human, chicken
application :
western blot, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 33
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; human; 1:1500; loading ...; fig 3c
Bianchi V, Weber J, Waldman S, Backstein D, Kandel R. Formation of Hyaline Cartilage Tissue by Passaged Human Osteoarthritic Chondrocytes. Tissue Eng Part A. 2017;23:156-165 pubmed publisher
  • western blot; chicken; 1:800
Juhász T, Szentléleky E, Somogyi C, Takács R, Dobrosi N, Engler M, et al. Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) Pathway Is Induced by Mechanical Load and Reduces the Activity of Hedgehog Signaling in Chondrogenic Micromass Cell Cultures. Int J Mol Sci. 2015;16:17344-67 pubmed publisher
  • immunohistochemistry - paraffin section; human
Pei M, Li J, Zhang Y, Liu G, Wei L, Zhang Y. Expansion on a matrix deposited by nonchondrogenic urine stem cells strengthens the chondrogenic capacity of repeated-passage bone marrow stromal cells. Cell Tissue Res. 2014;356:391-403 pubmed publisher
Chubinskaya S, Di Matteo B, Lovato L, Iacono F, Robinson D, Kon E. Agili-C implant promotes the regenerative capacity of articular cartilage defects in an ex vivo model. Knee Surg Sports Traumatol Arthrosc. 2019;27:1953-1964 pubmed publisher
Huynh N, Zhang B, Guilak F. High-depth transcriptomic profiling reveals the temporal gene signature of human mesenchymal stem cells during chondrogenesis. FASEB J. 2019;33:358-372 pubmed publisher
Endo K, Fujita N, Nakagawa T, Nishimura R. Effect of fibroblast growth factor-2 and serum on canine mesenchymal stem cell chondrogenesis. Tissue Eng Part A. 2018;: pubmed publisher
Rowland C, Glass K, Ettyreddy A, Gloss C, Matthews J, Huynh N, et al. Regulation of decellularized tissue remodeling via scaffold-mediated lentiviral delivery in anatomically-shaped osteochondral constructs. Biomaterials. 2018;177:161-175 pubmed publisher
Schmal H, Kowal J, Kassem M, Seidenstuecker M, Bernstein A, Böttiger K, et al. Comparison of Regenerative Tissue Quality following Matrix-Associated Cell Implantation Using Amplified Chondrocytes Compared to Synovium-Derived Stem Cells in a Rabbit Model for Cartilage Lesions. Stem Cells Int. 2018;2018:4142031 pubmed publisher
Huynh N, Brunger J, Gloss C, Moutos F, Gersbach C, Guilak F. Genetic Engineering of Mesenchymal Stem Cells for Differential Matrix Deposition on 3D Woven Scaffolds. Tissue Eng Part A. 2018;24:1531-1544 pubmed publisher
Valiani A, Izadi M, Bahramian H, Esfandiari E, Hashemibeni B. Comparison between the effect of kartogenin and TGFβ3 on chondrogenesis of human adipose- derived stem cells in fibrin scaffold. Bratisl Lek Listy. 2017;118:591-597 pubmed publisher
Gardner O, Musumeci G, Neumann A, Eglin D, Archer C, Alini M, et al. Asymmetrical seeding of MSCs into fibrin-poly(ester-urethane) scaffolds and its effect on mechanically induced chondrogenesis. J Tissue Eng Regen Med. 2017;11:2912-2921 pubmed publisher
Rowland C, Colucci L, Guilak F. Fabrication of anatomically-shaped cartilage constructs using decellularized cartilage-derived matrix scaffolds. Biomaterials. 2016;91:57-72 pubmed publisher
Mellor L, Mohiti Asli M, Williams J, Kannan A, Dent M, Guilak F, et al. Extracellular Calcium Modulates Chondrogenic and Osteogenic Differentiation of Human Adipose-Derived Stem Cells: A Novel Approach for Osteochondral Tissue Engineering Using a Single Stem Cell Source. Tissue Eng Part A. 2015;21:2323-33 pubmed publisher
Diekman B, Thakore P, O Connor S, Willard V, Brunger J, Christoforou N, et al. Knockdown of the cell cycle inhibitor p21 enhances cartilage formation by induced pluripotent stem cells. Tissue Eng Part A. 2015;21:1261-74 pubmed publisher
Sieker J, Kunz M, Weißenberger M, Gilbert F, Frey S, Rudert M, et al. Direct bone morphogenetic protein 2 and Indian hedgehog gene transfer for articular cartilage repair using bone marrow coagulates. Osteoarthritis Cartilage. 2015;23:433-42 pubmed publisher
Yoshizawa S, Brown A, Barchowsky A, Sfeir C. Role of magnesium ions on osteogenic response in bone marrow stromal cells. Connect Tissue Res. 2014;55 Suppl 1:155-9 pubmed publisher
Glass K, Link J, Brunger J, Moutos F, Gersbach C, Guilak F. Tissue-engineered cartilage with inducible and tunable immunomodulatory properties. Biomaterials. 2014;35:5921-31 pubmed publisher
Brunger J, Huynh N, Guenther C, Perez Pinera P, Moutos F, Sanchez Adams J, et al. Scaffold-mediated lentiviral transduction for functional tissue engineering of cartilage. Proc Natl Acad Sci U S A. 2014;111:E798-806 pubmed publisher
Mardani M, Kabiri A, Esfandiari E, Esmaeili A, Pourazar A, Ansar M, et al. The effect of platelet rich plasma on chondrogenic differentiation of human adipose derived stem cells in transwell culture. Iran J Basic Med Sci. 2013;16:1163-9 pubmed
Rowland C, Lennon D, Caplan A, Guilak F. The effects of crosslinking of scaffolds engineered from cartilage ECM on the chondrogenic differentiation of MSCs. Biomaterials. 2013;34:5802-12 pubmed publisher
Cheng N, Estes B, Young T, Guilak F. Genipin-crosslinked cartilage-derived matrix as a scaffold for human adipose-derived stem cell chondrogenesis. Tissue Eng Part A. 2013;19:484-96 pubmed publisher
Tran S, Cooley A, Elder S. Effect of a mechanical stimulation bioreactor on tissue engineered, scaffold-free cartilage. Biotechnol Bioeng. 2011;108:1421-9 pubmed publisher
Cheng N, Estes B, Young T, Guilak F. Engineered cartilage using primary chondrocytes cultured in a porous cartilage-derived matrix. Regen Med. 2011;6:81-93 pubmed publisher
Bernstein P, Sticht C, Jacobi A, Liebers C, Manthey S, Stiehler M. Expression pattern differences between osteoarthritic chondrocytes and mesenchymal stem cells during chondrogenic differentiation. Osteoarthritis Cartilage. 2010;18:1596-607 pubmed publisher
Estes B, Diekman B, Gimble J, Guilak F. Isolation of adipose-derived stem cells and their induction to a chondrogenic phenotype. Nat Protoc. 2010;5:1294-311 pubmed publisher
Ahmad P, Trcka D, Xue S, Franco C, Speer M, Giachelli C, et al. Discoidin domain receptor-1 deficiency attenuates atherosclerotic calcification and smooth muscle cell-mediated mineralization. Am J Pathol. 2009;175:2686-96 pubmed publisher
Diekman B, Estes B, Guilak F. The effects of BMP6 overexpression on adipose stem cell chondrogenesis: Interactions with dexamethasone and exogenous growth factors. J Biomed Mater Res A. 2010;93:994-1003 pubmed publisher
Diekman B, Rowland C, Lennon D, Caplan A, Guilak F. Chondrogenesis of adult stem cells from adipose tissue and bone marrow: induction by growth factors and cartilage-derived matrix. Tissue Eng Part A. 2010;16:523-33 pubmed publisher
Khan A, Suits J, Kandel R, Waldman S. The effect of continuous culture on the growth and structure of tissue-engineered cartilage. Biotechnol Prog. 2009;25:508-15 pubmed publisher
Kawakita A, Sato K, Makino H, Ikegami H, Takayama S, Toyama Y, et al. Nicotine acts on growth plate chondrocytes to delay skeletal growth through the alpha7 neuronal nicotinic acetylcholine receptor. PLoS ONE. 2008;3:e3945 pubmed publisher
Cheng N, Estes B, Awad H, Guilak F. Chondrogenic differentiation of adipose-derived adult stem cells by a porous scaffold derived from native articular cartilage extracellular matrix. Tissue Eng Part A. 2009;15:231-41 pubmed publisher
Ulici V, Hoenselaar K, Gillespie J, Beier F. The PI3K pathway regulates endochondral bone growth through control of hypertrophic chondrocyte differentiation. BMC Dev Biol. 2008;8:40 pubmed publisher
Wang G, Woods A, Agoston H, Ulici V, Glogauer M, Beier F. Genetic ablation of Rac1 in cartilage results in chondrodysplasia. Dev Biol. 2007;306:612-23 pubmed
product information
Product No. :
C7974
Product Name :
Monoclonal Anti-Collagen, Type X antibody produced in mouse
Host :
mouse
Conjugate :
unconjugated
Antibody Type :
monoclonal
Clone Number (if monoclonal) :
COL-10
Applications/Description :
Suitable for dot blot; western blot; immunocytochemistry: 1:1,000 using HT 1080 human fibrosarcoma cells
Species Reactivity :
deer, pig, human
Isotype :
IgM
Storage :
Store at -20°C
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA