product summary
company name :
MilliporeSigma
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, FLUKA, Sigma-Aldrich, Novabiochem, Guava, Roche Applied Science
product type :
antibody
product name :
Monoclonal Anti-β-Catenin antibody produced in mouse
catalog :
C7207
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
15B8
reactivity :
human, mouse, rat, dogs, chicken, bovine, zebrafish
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 78
Published Application/Species/Sample/DilutionReference
  • western blot; rat; loading ...; fig 5a
Wang H, Segersv xe4 rd H, Siren J, Perttunen S, Immonen K, Kosonen R, et al. Tankyrase Inhibition Attenuates Cardiac Dilatation and Dysfunction in Ischemic Heart Failure. Int J Mol Sci. 2022;23: pubmed publisher
  • western blot; mouse; 1:2000; loading ...; fig 2f
  • immunocytochemistry; human; 1:500; loading ...; fig s1-2
Gumber D, Do M, Suresh Kumar N, Sonavane P, Wu C, Cruz L, et al. Selective activation of FZD7 promotes mesendodermal differentiation of human pluripotent stem cells. elife. 2020;9: pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:1000; loading ...; fig 1s1a
Matos I, Asare A, Levorse J, Ouspenskaia T, de la Cruz Racelis J, Schuhmacher L, et al. Progenitors oppositely polarize WNT activators and inhibitors to orchestrate tissue development. elife. 2020;9: pubmed publisher
  • immunohistochemistry; zebrafish ; 1:500; loading ...; fig 4a
Schauer A, Pinheiro D, Hauschild R, Heisenberg C. Zebrafish embryonic explants undergo genetically encoded self-assembly. elife. 2020;9: pubmed publisher
  • immunocytochemistry; mouse; loading ...; fig 2b
Manalo A, Schroer A, Fenix A, Shancer Z, Coogan J, Brolsma T, et al. Loss of CENP-F Results in Dilated Cardiomyopathy with Severe Disruption of Cardiac Myocyte Architecture. Sci Rep. 2018;8:7546 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:1000; loading ...; fig s2d
Yousefi M, Li N, Nakauka Ddamba A, Wang S, Davidow K, Schoenberger J, et al. Msi RNA-binding proteins control reserve intestinal stem cell quiescence. J Cell Biol. 2016;215:401-413 pubmed
  • immunohistochemistry - paraffin section; mouse; 1:500; loading ...
Freddo A, Shoffner S, Shao Y, Taniguchi K, Grosse A, Guysinger M, et al. Coordination of signaling and tissue mechanics during morphogenesis of murine intestinal villi: a role for mitotic cell rounding. Integr Biol (Camb). 2016;8:918-28 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:1000; fig 1
Li N, Yousefi M, Nakauka Ddamba A, Tobias J, Jensen S, Morrisey E, et al. Heterogeneity in readouts of canonical wnt pathway activity within intestinal crypts. Dev Dyn. 2016;245:822-33 pubmed publisher
  • immunoprecipitation; human; fig 5
  • immunoprecipitation; mouse; fig 6
Kumar A, Chalamalasetty R, Kennedy M, Thomas S, Inala S, Garriock R, et al. Zfp703 Is a Wnt/?-Catenin Feedback Suppressor Targeting the ?-Catenin/Tcf1 Complex. Mol Cell Biol. 2016;36:1793-802 pubmed publisher
  • immunocytochemistry; human; 1:250; fig s13b
  • western blot; human; 1:1000; fig 8e
Lin K, Kao S, Lai C, Chen C, Wu C, Hsu H, et al. Tumor Suppressor Lzap Suppresses Wnt/β-Catenin Signaling to Promote Zebrafish Embryonic Ventral Cell Fates via the Suppression of Inhibitory Phosphorylation of Glycogen Synthase Kinase 3. J Biol Chem. 2015;290:29808-19 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; fig 2
Bebee T, Park J, Sheridan K, Warzecha C, Cieply B, Rohacek A, et al. The splicing regulators Esrp1 and Esrp2 direct an epithelial splicing program essential for mammalian development. elife. 2015;4: pubmed publisher
  • immunohistochemistry - frozen section; zebrafish ; fig s1
  • immunohistochemistry; zebrafish ; 1:200; fig s1
Evason K, Francisco M, Juric V, Balakrishnan S, Lopez Pazmino M, Gordan J, et al. Identification of Chemical Inhibitors of β-Catenin-Driven Liver Tumorigenesis in Zebrafish. PLoS Genet. 2015;11:e1005305 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:150
Remo A, Simeone I, Pancione M, Parcesepe P, Finetti P, Cerulo L, et al. Systems biology analysis reveals NFAT5 as a novel biomarker and master regulator of inflammatory breast cancer. J Transl Med. 2015;13:138 pubmed publisher
  • immunohistochemistry; chicken; 1:200; fig 2
  • western blot; chicken; 1:2000; fig 5
Herrera A, Saade M, Menendez A, Marti E, Pons S. Sustained Wnt/?-catenin signalling causes neuroepithelial aberrations through the accumulation of aPKC at the apical pole. Nat Commun. 2014;5:4168 pubmed publisher
  • immunocytochemistry; mouse; fig 1
Okumura N, Akutsu H, Sugawara T, Miura T, Takezawa Y, Hosoda A, et al. ?-catenin functions pleiotropically in differentiation and tumorigenesis in mouse embryo-derived stem cells. PLoS ONE. 2013;8:e63265 pubmed publisher
  • immunohistochemistry; mouse
Nair M, Nagamori I, Sun P, Mishra D, Rhéaume C, Li B, et al. Nuclear regulator Pygo2 controls spermiogenesis and histone H3 acetylation. Dev Biol. 2008;320:446-55 pubmed publisher
Low J, Du W, Gocha T, Oguz G, Zhang X, Chen M, et al. Molecular docking-aided identification of small molecule inhibitors targeting β-catenin-TCF4 interaction. iScience. 2021;24:102544 pubmed publisher
Weber C, Zhou Y, Lee J, Looger L, Qian G, Ge C, et al. Temperature-dependent sex determination is mediated by pSTAT3 repression of Kdm6b. Science. 2020;368:303-306 pubmed publisher
Unlu G, Qi X, Gamazon E, Melville D, Patel N, Rushing A, et al. Phenome-based approach identifies RIC1-linked Mendelian syndrome through zebrafish models, biobank associations and clinical studies. Nat Med. 2020;26:98-109 pubmed publisher
Young R, Ewan K, Ferrer V, Allende M, Godovac Zimmermann J, Dale T, et al. Developmentally regulated Tcf7l2 splice variants mediate transcriptional repressor functions during eye formation. elife. 2019;8: pubmed publisher
Akieda Y, Ogamino S, Furuie H, Ishitani S, Akiyoshi R, Nogami J, et al. Cell competition corrects noisy Wnt morphogen gradients to achieve robust patterning in the zebrafish embryo. Nat Commun. 2019;10:4710 pubmed publisher
Kalasekar S, Kotiyal S, Conley C, Phan C, Young A, Evason K. Heterogeneous beta-catenin activation is sufficient to cause hepatocellular carcinoma in zebrafish. Biol Open. 2019;8: pubmed publisher
Juettner V, Kruse K, Dan A, Vu V, Khan Y, Le J, et al. VE-PTP stabilizes VE-cadherin junctions and the endothelial barrier via a phosphatase-independent mechanism. J Cell Biol. 2019;218:1725-1742 pubmed publisher
Yin X, Liu Z, Zhu P, Wang Y, Ren Q, Chen H, et al. CXCL12/CXCR4 promotes proliferation, migration, and invasion of adamantinomatous craniopharyngiomas via PI3K/AKT signal pathway. J Cell Biochem. 2019;120:9724-9736 pubmed publisher
Song Y, Boncompagni A, Kim S, Gochnauer H, Zhang Y, Loots G, et al. Regional Control of Hairless versus Hair-Bearing Skin by Dkk2. Cell Rep. 2018;25:2981-2991.e3 pubmed publisher
Jang S, Cho I, Yang J, Park H, Woo S, Madrakhimov S, et al. The retinal pigment epithelial response after retinal laser photocoagulation in diabetic mice. Lasers Med Sci. 2019;34:179-190 pubmed publisher
Xing Y, Cheng X, Li Y, Zhang C, Saquet A, Liu Y, et al. Mutational analysis of dishevelled genes in zebrafish reveals distinct functions in embryonic patterning and gastrulation cell movements. PLoS Genet. 2018;14:e1007551 pubmed publisher
Pan J, Xiong L, Zhao K, Zeng P, Wang B, Tang F, et al. YAP promotes osteogenesis and suppresses adipogenic differentiation by regulating β-catenin signaling. Bone Res. 2018;6:18 pubmed publisher
Sun Q, Rabbani P, Takeo M, Lee S, Lim C, Noel E, et al. Dissecting Wnt Signaling for Melanocyte Regulation during Wound Healing. J Invest Dermatol. 2018;138:1591-1600 pubmed publisher
Huggins I, Bos T, Gaylord O, Jessen C, Lonquich B, Puranen A, et al. The WNT target SP5 negatively regulates WNT transcriptional programs in human pluripotent stem cells. Nat Commun. 2017;8:1034 pubmed publisher
Yasumuro H, Sakurai K, Toyama F, Maruo F, Chiba C. Implications of a Multi-Step Trigger of Retinal Regeneration in the Adult Newt. Biomedicines. 2017;5: pubmed publisher
Shimizu K, Fukushima H, Ogura K, Lien E, Nihira N, Zhang J, et al. The SCF?-TRCP E3 ubiquitin ligase complex targets Lipin1 for ubiquitination and degradation to promote hepatic lipogenesis. Sci Signal. 2017;10: pubmed publisher
Rydeen A, Waxman J. Cyp26 Enzymes Facilitate Second Heart Field Progenitor Addition and Maintenance of Ventricular Integrity. PLoS Biol. 2016;14:e2000504 pubmed publisher
Sarmah S, Muralidharan P, Marrs J. Embryonic Ethanol Exposure Dysregulates BMP and Notch Signaling, Leading to Persistent Atrio-Ventricular Valve Defects in Zebrafish. PLoS ONE. 2016;11:e0161205 pubmed publisher
Chou C, Hsu H, You M, Lin J, Liu Y. The endoderm indirectly influences morphogenetic movements of the zebrafish head kidney through the posterior cardinal vein and VegfC. Sci Rep. 2016;6:30677 pubmed publisher
Rabadán M, Herrera A, Fanlo L, Usieto S, Carmona Fontaine C, Barriga E, et al. Delamination of neural crest cells requires transient and reversible Wnt inhibition mediated by Dact1/2. Development. 2016;143:2194-205 pubmed publisher
Takeo M, Hale C, Ito M. Epithelium-Derived Wnt Ligands Are Essential for Maintenance of Underlying Digit Bone. J Invest Dermatol. 2016;136:1355-1363 pubmed publisher
Verstraeten B, van Hengel J, Huysseune A. Beta-Catenin and Plakoglobin Expression during Zebrafish Tooth Development and Replacement. PLoS ONE. 2016;11:e0148114 pubmed publisher
Xu Z, Wang W, Jiang K, Yu Z, Huang H, Wang F, et al. Embryonic attenuated Wnt/β-catenin signaling defines niche location and long-term stem cell fate in hair follicle. elife. 2015;4:e10567 pubmed publisher
Li Villarreal N, Forbes M, Loza A, Chen J, Ma T, Helde K, et al. Dachsous1b cadherin regulates actin and microtubule cytoskeleton during early zebrafish embryogenesis. Development. 2015;142:2704-18 pubmed publisher
Roszko I, S Sepich D, Jessen J, Chandrasekhar A, Solnica Krezel L. A dynamic intracellular distribution of Vangl2 accompanies cell polarization during zebrafish gastrulation. Development. 2015;142:2508-20 pubmed publisher
Vidal S, Amlani B, Chen T, Tsirigos A, Stadtfeld M. Combinatorial modulation of signaling pathways reveals cell-type-specific requirements for highly efficient and synchronous iPSC reprogramming. Stem Cell Reports. 2014;3:574-84 pubmed publisher
Wang S, Celic I, Choi S, Riccomagno M, Wang Q, Sun L, et al. Dlg5 regulates dendritic spine formation and synaptogenesis by controlling subcellular N-cadherin localization. J Neurosci. 2014;34:12745-61 pubmed publisher
Willis C, Klüppel M. Chondroitin sulfate-E is a negative regulator of a pro-tumorigenic Wnt/beta-catenin-Collagen 1 axis in breast cancer cells. PLoS ONE. 2014;9:e103966 pubmed publisher
Pattabiraman P, Epstein D, Rao P. Regulation of Adherens Junctions in Trabecular Meshwork Cells by Rac GTPase and their influence on Intraocular Pressure. J Ocul Biol. 2013;1: pubmed
Nicot A, Lo Verso F, Ratti F, Pilot Storck F, Streichenberger N, Sandri M, et al. Phosphorylation of NBR1 by GSK3 modulates protein aggregation. Autophagy. 2014;10:1036-53 pubmed publisher
Mermejo L, Leal L, Colli L, Fragoso M, Latronico A, Tone L, et al. Altered expression of noncanonical Wnt pathway genes in paediatric and adult adrenocortical tumours. Clin Endocrinol (Oxf). 2014;81:503-10 pubmed publisher
Pink A, Kallastu A, Turkina M, Skolnaja M, Kogerman P, Päll T, et al. Purification, characterization and plasma half-life of PEGylated soluble recombinant non-HA-binding CD44. BioDrugs. 2014;28:393-402 pubmed publisher
Lien W, Polak L, Lin M, Lay K, Zheng D, Fuchs E. In vivo transcriptional governance of hair follicle stem cells by canonical Wnt regulators. Nat Cell Biol. 2014;16:179-90 pubmed publisher
Castelli M, Boca M, Chiaravalli M, Ramalingam H, Rowe I, Distefano G, et al. Polycystin-1 binds Par3/aPKC and controls convergent extension during renal tubular morphogenesis. Nat Commun. 2013;4:2658 pubmed publisher
Corallo D, Schiavinato A, Trapani V, Moro E, Argenton F, Bonaldo P. Emilin3 is required for notochord sheath integrity and interacts with Scube2 to regulate notochord-derived Hedgehog signals. Development. 2013;140:4594-601 pubmed publisher
Weiser K, Barton M, Gershoony D, DasGupta R, Cardozo T. HIV's Nef interacts with β-catenin of the Wnt signaling pathway in HEK293 cells. PLoS ONE. 2013;8:e77865 pubmed publisher
Yang Z, Hai B, Qin L, Ti X, Shangguan L, Zhao Y, et al. Cessation of epithelial Bmp signaling switches the differentiation of crown epithelia to the root lineage in a ?-catenin-dependent manner. Mol Cell Biol. 2013;33:4732-44 pubmed publisher
Schumacher J, Bloomekatz J, Garavito Aguilar Z, Yelon D. tal1 Regulates the formation of intercellular junctions and the maintenance of identity in the endocardium. Dev Biol. 2013;383:214-26 pubmed publisher
Chou W, Takeo M, Rabbani P, Hu H, Lee W, Chung Y, et al. Direct migration of follicular melanocyte stem cells to the epidermis after wounding or UVB irradiation is dependent on Mc1r signaling. Nat Med. 2013;19:924-9 pubmed publisher
Jordan N, Prat A, Abell A, Zawistowski J, Sciaky N, Karginova O, et al. SWI/SNF chromatin-remodeling factor Smarcd3/Baf60c controls epithelial-mesenchymal transition by inducing Wnt5a signaling. Mol Cell Biol. 2013;33:3011-25 pubmed publisher
Song S, Eckerle S, Onichtchouk D, Marrs J, Nitschke R, Driever W. Pou5f1-dependent EGF expression controls E-cadherin endocytosis, cell adhesion, and zebrafish epiboly movements. Dev Cell. 2013;24:486-501 pubmed publisher
Mureli S, Gans C, Bare D, Geenen D, Kumar N, Banach K. Mesenchymal stem cells improve cardiac conduction by upregulation of connexin 43 through paracrine signaling. Am J Physiol Heart Circ Physiol. 2013;304:H600-9 pubmed publisher
Wu S, Shin J, Sepich D, Solnica Krezel L. Chemokine GPCR signaling inhibits β-catenin during zebrafish axis formation. PLoS Biol. 2012;10:e1001403 pubmed publisher
Syu L, El Zaatari M, Eaton K, Liu Z, Tetarbe M, Keeley T, et al. Transgenic expression of interferon-? in mouse stomach leads to inflammation, metaplasia, and dysplasia. Am J Pathol. 2012;181:2114-25 pubmed publisher
Asensio Juan E, Gallego C, Martínez Balbás M. The histone demethylase PHF8 is essential for cytoskeleton dynamics. Nucleic Acids Res. 2012;40:9429-40 pubmed publisher
Zhou B, Liu Y, Kahn M, Ann D, Han A, Wang H, et al. Interactions between ?-catenin and transforming growth factor-? signaling pathways mediate epithelial-mesenchymal transition and are dependent on the transcriptional co-activator cAMP-response element-binding protein (CREB)-binding protein (CBP). J Biol Chem. 2012;287:7026-38 pubmed publisher
Anton R, Chatterjee S, Simundza J, Cowin P, DasGupta R. A systematic screen for micro-RNAs regulating the canonical Wnt pathway. PLoS ONE. 2011;6:e26257 pubmed publisher
Gonsalves F, Klein K, Carson B, Katz S, Ekas L, Evans S, et al. An RNAi-based chemical genetic screen identifies three small-molecule inhibitors of the Wnt/wingless signaling pathway. Proc Natl Acad Sci U S A. 2011;108:5954-63 pubmed publisher
Inuzuka H, Shaik S, Onoyama I, Gao D, Tseng A, Maser R, et al. SCF(FBW7) regulates cellular apoptosis by targeting MCL1 for ubiquitylation and destruction. Nature. 2011;471:104-9 pubmed publisher
Garavito Aguilar Z, Riley H, Yelon D. Hand2 ensures an appropriate environment for cardiac fusion by limiting Fibronectin function. Development. 2010;137:3215-20 pubmed publisher
Verstraeten B, Sanders E, van Hengel J, Huysseune A. Zebrafish teeth as a model for repetitive epithelial morphogenesis: dynamics of E-cadherin expression. BMC Dev Biol. 2010;10:58 pubmed publisher
Freyer L, Morrow B. Canonical Wnt signaling modulates Tbx1, Eya1, and Six1 expression, restricting neurogenesis in the otic vesicle. Dev Dyn. 2010;239:1708-22 pubmed publisher
Peterson M, Henry C. Hedgehog signaling and laminin play unique and synergistic roles in muscle development. Dev Dyn. 2010;239:905-13 pubmed publisher
Mill P, Lee A, Fukata Y, Tsutsumi R, Fukata M, Keighren M, et al. Palmitoylation regulates epidermal homeostasis and hair follicle differentiation. PLoS Genet. 2009;5:e1000748 pubmed publisher
Muraoka Cook R, Sandahl M, Strunk K, Miraglia L, Husted C, Hunter D, et al. ErbB4 splice variants Cyt1 and Cyt2 differ by 16 amino acids and exert opposing effects on the mammary epithelium in vivo. Mol Cell Biol. 2009;29:4935-48 pubmed publisher
Snow C, Peterson M, Khalil A, Henry C. Muscle development is disrupted in zebrafish embryos deficient for fibronectin. Dev Dyn. 2008;237:2542-53 pubmed publisher
Marose T, Merkel C, McMahon A, Carroll T. Beta-catenin is necessary to keep cells of ureteric bud/Wolffian duct epithelium in a precursor state. Dev Biol. 2008;314:112-26 pubmed publisher
Guidato S, Itasaki N. Wise retained in the endoplasmic reticulum inhibits Wnt signaling by reducing cell surface LRP6. Dev Biol. 2007;310:250-63 pubmed
Shi C, Huang N, Harrison K, Han S, Kehrl J. The mitogen-activated protein kinase kinase kinase kinase GCKR positively regulates canonical and noncanonical Wnt signaling in B lymphocytes. Mol Cell Biol. 2006;26:6511-21 pubmed
Henry C, McNulty I, Durst W, Munchel S, Amacher S. Interactions between muscle fibers and segment boundaries in zebrafish. Dev Biol. 2005;287:346-60 pubmed
Yasui K, Li G, Wang Y, Saiga H, Zhang P, Aizawa S. beta-Catenin in early development of the lancelet embryo indicates specific determination of embryonic polarity. Dev Growth Differ. 2002;44:467-75 pubmed
Johnson K, Lewis J, Li D, Wahl J, Soler A, Knudsen K, et al. P- and E-cadherin are in separate complexes in cells expressing both cadherins. Exp Cell Res. 1993;207:252-60 pubmed
product information
Product No. :
C7207
Product Name :
Monoclonal Anti-β-Catenin antibody produced in mouse
Host :
mouse
Conjugate :
unconjugated
Antibody Type :
monoclonal
Clone Number (if monoclonal) :
15B8
Applications/Description :
Suitable for immunocytochemistry; western blot: 1:1000 using cultured MDBK cells extract; microarray; indirect immunofluorescence: 1:1000 using cultured MDBK cells; immunohistochemistry (frozen sections)
Species Reactivity :
bovine, canine, human, chicken
Isotype :
IgG1
Storage :
Store at -20°C
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA