product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
antibody
product name :
Monoclonal Anti-β-Tubulin−Cy3 antibody produced in mouse
catalog :
C4585
clonality :
monoclonal
host :
mouse
conjugate :
Cy3
clone name :
TUB 2.1
reactivity :
human, mouse, rat, chicken, bovine, pigs
application :
western blot, immunohistochemistry, immunocytochemistry
more info or order :
citations: 34
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse; 1:200; fig 3b
Raju D, Schonauer S, Hamzeh H, FLYNN K, Bradke F, Vom Dorp K, et al. Accumulation of glucosylceramide in the absence of the beta-glucosidase GBA2 alters cytoskeletal dynamics. PLoS Genet. 2015;11:e1005063 pubmed publisher
  • western blot; mouse; 1:10000; fig 1
Tokuda E, Watanabe S, Okawa E, Ono S. Regulation of Intracellular Copper by Induction of Endogenous Metallothioneins Improves the Disease Course in a Mouse Model of Amyotrophic Lateral Sclerosis. Neurotherapeutics. 2015;12:461-76 pubmed publisher
  • immunocytochemistry; pigs ; fig 2
Cai Y, Fedeles S, Dong K, Anyatonwu G, Onoe T, Mitobe M, et al. Altered trafficking and stability of polycystins underlie polycystic kidney disease. J Clin Invest. 2014;124:5129-44 pubmed publisher
  • western blot; human
Srinivasan S, Romagnoli M, Bohm A, Sonenshein G. N-glycosylation regulates ADAM8 processing and activation. J Biol Chem. 2014;289:33676-88 pubmed publisher
  • western blot; mouse
De Rossi G, Evans A, Kay E, Woodfin A, McKay T, Nourshargh S, et al. Shed syndecan-2 inhibits angiogenesis. J Cell Sci. 2014;127:4788-99 pubmed publisher
  • western blot; mouse
McGowan S, McCoy D. Regulation of fibroblast lipid storage and myofibroblast phenotypes during alveolar septation in mice. Am J Physiol Lung Cell Mol Physiol. 2014;307:L618-31 pubmed publisher
  • western blot; human; fig 3
McEwen A, Maher M, Mo R, Gottardi C. E-cadherin phosphorylation occurs during its biosynthesis to promote its cell surface stability and adhesion. Mol Biol Cell. 2014;25:2365-74 pubmed publisher
  • western blot; human
Tsika E, Glauser L, Moser R, Fiser A, Daniel G, Sheerin U, et al. Parkinson's disease-linked mutations in VPS35 induce dopaminergic neurodegeneration. Hum Mol Genet. 2014;23:4621-38 pubmed publisher
  • western blot; rat
Han X, Cheng D, Song F, Zeng T, An L, Xie K. Decelerated transport and its mechanism of 2,5-hexanedione on middle-molecular-weight neurofilament in rat dorsal root ganglia cells. Neuroscience. 2014;269:192-8 pubmed publisher
  • immunocytochemistry; mouse
Jung H, Song K, Chang J, Doh J. Geometrically controlled asymmetric division of CD4+ T cells studied by immunological synapse arrays. PLoS ONE. 2014;9:e91926 pubmed publisher
  • western blot; human
Yim D, Ghosh S, Guy G, Virshup D. Casein kinase 1 regulates Sprouty2 in FGF-ERK signaling. Oncogene. 2015;34:474-84 pubmed publisher
  • immunocytochemistry; human; 1:200
Körschen H, Yildiz Y, Raju D, Schonauer S, Bönigk W, Jansen V, et al. The non-lysosomal ?-glucosidase GBA2 is a non-integral membrane-associated protein at the endoplasmic reticulum (ER) and Golgi. J Biol Chem. 2013;288:3381-93 pubmed publisher
Lobo Jarne T, Nyvltová E, Pérez Pérez R, Timón Gómez A, Molinié T, Choi A, et al. Human COX7A2L Regulates Complex III Biogenesis and Promotes Supercomplex Organization Remodeling without Affecting Mitochondrial Bioenergetics. Cell Rep. 2018;25:1786-1799.e4 pubmed publisher
Guidi L, Holloway Z, Arnoult C, Ray P, Monaco A, Molnar Z, et al. AU040320 deficiency leads to disruption of acrosome biogenesis and infertility in homozygous mutant mice. Sci Rep. 2018;8:10379 pubmed publisher
Huang X, Kong W, Qiao J. Response of the Retinal Nerve Fiber Layer Reflectance and Thickness to Optic Nerve Crush. Invest Ophthalmol Vis Sci. 2018;59:2094-2103 pubmed publisher
Park J, Kwon M, Kim E, Lee H, Song E. USP35 regulates mitotic progression by modulating the stability of Aurora B. Nat Commun. 2018;9:688 pubmed publisher
Jakka P, Bhargavi B, Namani S, Murugan S, Splitter G, Radhakrishnan G. Cytoplasmic Linker Protein CLIP170 Negatively Regulates TLR4 Signaling by Targeting the TLR Adaptor Protein TIRAP. J Immunol. 2018;200:704-714 pubmed publisher
Murray J, Yin D, Wolkoff A. Reduction of organelle motility by removal of potassium and other solutes. PLoS ONE. 2017;12:e0184898 pubmed publisher
Barbiero I, Valente D, Chandola C, Magi F, Bergo A, Monteonofrio L, et al. CDKL5 localizes at the centrosome and midbody and is required for faithful cell division. Sci Rep. 2017;7:6228 pubmed publisher
Hung S, Lesmana A, Peck A, Lee R, Tchoubrieva E, Hannan K, et al. Cell cycle and growth stimuli regulate different steps of RNA polymerase I transcription. Gene. 2017;612:36-48 pubmed publisher
Liu K, Qiu W, Naveen Raj E, Liu H, Huang H, Lin Y, et al. Ubiquitin-coated nanodiamonds bind to autophagy receptors for entry into the selective autophagy pathway. Autophagy. 2017;13:187-200 pubmed publisher
Janmaleki M, Pachenari M, Seyedpour S, Shahghadami R, Sanati Nezhad A. Impact of Simulated Microgravity on Cytoskeleton and Viscoelastic Properties of Endothelial Cell. Sci Rep. 2016;6:32418 pubmed publisher
Seno T, Ikeno T, Mennya K, Kurishita M, Sakae N, Sato M, et al. Kinesin-1 sorting in axons controls the differential retraction of arbor terminals. J Cell Sci. 2016;129:3499-510 pubmed publisher
Delgado Ortega M, Olivier M, Sizaret P, Simon G, Meurens F. Newborn pig trachea cell line cultured in air-liquid interface conditions allows a partial in vitro representation of the porcine upper airway tissue. BMC Cell Biol. 2014;15:14 pubmed publisher
Brockmann C, Huarte J, Dugina V, Challet L, Rey E, Conne B, et al. Beta- and gamma-cytoplasmic actins are required for meiosis in mouse oocytes. Biol Reprod. 2011;85:1025-39 pubmed publisher
Quintanar Audelo M, Yusoff P, Sinniah S, Chandramouli S, Guy G. Sprouty-related Ena/vasodilator-stimulated phosphoprotein homology 1-domain-containing protein (SPRED1), a tyrosine-protein phosphatase non-receptor type 11 (SHP2) substrate in the Ras/extracellular signal-regulated kinase (ERK) pathway. J Biol Chem. 2011;286:23102-12 pubmed publisher
Liu M, Nadar V, Kozielski F, Kozlowska M, Yu W, Baas P. Kinesin-12, a mitotic microtubule-associated motor protein, impacts axonal growth, navigation, and branching. J Neurosci. 2010;30:14896-906 pubmed publisher
Qiang L, Yu W, Liu M, Solowska J, Baas P. Basic fibroblast growth factor elicits formation of interstitial axonal branches via enhanced severing of microtubules. Mol Biol Cell. 2010;21:334-44 pubmed publisher
Williamson A, Wickliffe K, Mellone B, Song L, Karpen G, Rape M. Identification of a physiological E2 module for the human anaphase-promoting complex. Proc Natl Acad Sci U S A. 2009;106:18213-8 pubmed publisher
Callis T, Pandya K, Seok H, Tang R, Tatsuguchi M, Huang Z, et al. MicroRNA-208a is a regulator of cardiac hypertrophy and conduction in mice. J Clin Invest. 2009;119:2772-86 pubmed publisher
Dong S, Hamel E, Bai R, Covell D, Beutler J, Porco J. Enantioselective synthesis of (+)-chamaecypanone C: a novel microtubule inhibitor. Angew Chem Int Ed Engl. 2009;48:1494-7 pubmed publisher
Yu W, Qiang L, Solowska J, Karabay A, Korulu S, Baas P. The microtubule-severing proteins spastin and katanin participate differently in the formation of axonal branches. Mol Biol Cell. 2008;19:1485-98 pubmed publisher
Yu W, Solowska J, Qiang L, Karabay A, Baird D, Baas P. Regulation of microtubule severing by katanin subunits during neuronal development. J Neurosci. 2005;25:5573-83 pubmed
Oca Cossio J, Kenyon L, Hao H, Moraes C. Limitations of allotopic expression of mitochondrial genes in mammalian cells. Genetics. 2003;165:707-20 pubmed
product information
Product No. :
C4585
Product Name :
Monoclonal Anti-β-Tubulin−Cy3 antibody produced in mouse
Host :
mouse
Conjugate :
CY3 conjugate
Antibody Type :
monoclonal
Clone Number (if monoclonal) :
TUB 2.1
Applications/Description :
Suitable for direct immunofluorescence: 1:100 using cultured chicken fibroblasts or BHK cells
Species Reactivity :
chicken, human, plant, frog, wheat, rabbit, bovine, hamster, moth, mouse, sea urchin, rat
Isotype :
IgG1
Storage :
Store at 2-8°C
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA