Anti-Atg12 antibody produced in rabbit | MilliporeSigma A8731 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MilliporeSigma

other brands :

FLUKA, Sigma-Aldrich, Roche Applied Science

product type :

antibody

product name :

Anti-Atg12 antibody produced in rabbit

catalog :

A8731

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunoprecipitation

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; loading ...; fig 1a	Tazi M, Dakhlallah D, Caution K, Gerber M, Chang S, Khalil H, et al. Elevated Mirc1/Mir17-92 cluster expression negatively regulates autophagy and CFTR (cystic fibrosis transmembrane conductance regulator) function in CF macrophages. Autophagy. 2016;12:2026-2037 pubmed

product information

cat no :

A8731

brand :

SIGMA

name :

Anti-Atg12 antibody produced in rabbit

prod type :

Chemical

name suffix :

IgG fraction of antiserum, buffered aqueous solution

storage :

-20C

storage temp :

-20C

physical form :

Solution in 0.01 M phos

shipped in :

dry ice

syns :

Anti-APG12; Anti-Autophagy related 12 homolog (S. cerevisiae)

unspsc code :

12352203

wgk :

form :

buffered aqueous solution

antibody form :

IgG fraction of antiserum

application(s) :

western blot: 1:250-1:500 using whole extract of HEK-293T cells expressing mouse Atg12; immunoprecipitation: 5-10 muL using whole extract of HEK-293T cells co-expressing mouse Atg12 and Atg5-EGFP proteins

species reactivity :

mouse

immunogen :

synthetic peptide corresponding to amino acids 27-39 of mouse Atg12, conjugated to KLH. The corresponding sequence differs by one amino acid in human.

mol wt :

antigen mol wt ~20 kDa