product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
antibody
product name :
Monoclonal Anti-α-Fetoprotein (AFP) antibody produced in mouse
catalog :
A8452
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
C3
reactivity :
African green monkey, human, pigs
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
more info or order :
citations: 62
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; fig s4a
  • western blot; human; fig s4d
Wu X, Dao Thi V, Huang Y, Billerbeck E, Saha D, Hoffmann H, et al. Intrinsic Immunity Shapes Viral Resistance of Stem Cells. Cell. 2018;172:423-438.e25 pubmed publisher
  • immunocytochemistry; human; 1:250; fig 1
Herring A, Messana A, Bara A, Hazelbaker D, Eggan K, Barrett L. Generation of a TLE1 homozygous knockout human embryonic stem cell line using CRISPR-Cas9. Stem Cell Res. 2016;17:430-432 pubmed publisher
  • immunocytochemistry; human; 1:1000; fig 1
Momcilovic O, Sivapatham R, Oron T, Meyer M, Mooney S, Rao M, et al. Derivation, Characterization, and Neural Differentiation of Integration-Free Induced Pluripotent Stem Cell Lines from Parkinson's Disease Patients Carrying SNCA, LRRK2, PARK2, and GBA Mutations. PLoS ONE. 2016;11:e0154890 pubmed publisher
  • immunocytochemistry; human; 1:330; fig 4
Carpentier A, Nimgaonkar I, Chu V, Xia Y, Hu Z, Liang T. Hepatic differentiation of human pluripotent stem cells in miniaturized format suitable for high-throughput screen. Stem Cell Res. 2016;16:640-50 pubmed publisher
  • immunohistochemistry; human; 1:400; fig 3b
Meng G, Poon A, Liu S, Rancourt D. An Effective and Reliable Xeno-free Cryopreservation Protocol for Single Human Pluripotent Stem Cells. Methods Mol Biol. 2016;1516:47-56 pubmed publisher
  • immunocytochemistry; human; 1:1000; fig 5s1e
Carroll B, Maetzel D, Maddocks O, Otten G, Ratcliff M, Smith G, et al. Control of TSC2-Rheb signaling axis by arginine regulates mTORC1 activity. elife. 2016;5: pubmed publisher
  • immunocytochemistry; pigs ; fig 3
Zhang Y, Ma J, Li H, Lv J, Wei R, Cong Y, et al. bFGF signaling-mediated reprogramming of porcine primordial germ cells. Cell Tissue Res. 2016;364:429-41 pubmed publisher
  • immunocytochemistry; African green monkey; 1:250; fig 1
Ramaswamy K, Yik W, Wang X, Oliphant E, Lu W, Shibata D, et al. Derivation of induced pluripotent stem cells from orangutan skin fibroblasts. BMC Res Notes. 2015;8:577 pubmed publisher
  • immunoprecipitation; human; 1:500; fig 1g
Yu J, Ramasamy T, Murphy N, Holt M, Czapiewski R, Wei S, et al. PI3K/mTORC2 regulates TGF-β/Activin signalling by modulating Smad2/3 activity via linker phosphorylation. Nat Commun. 2015;6:7212 pubmed publisher
  • immunocytochemistry; human; 1:600; tbl 1
Yanagida A, Nakauchi H, Kamiya A. Generation and In Vitro Expansion of Hepatic Progenitor Cells from Human iPS Cells. Methods Mol Biol. 2016;1357:295-310 pubmed publisher
  • immunocytochemistry; human
Sivapatham R, Zeng X. Generation and Characterization of Patient-Specific Induced Pluripotent Stem Cell for Disease Modeling. Methods Mol Biol. 2016;1353:25-44 pubmed publisher
  • immunocytochemistry; human; 1:500
Zhu S, Rezvani M, Harbell J, Mattis A, Wolfe A, Benet L, et al. Mouse liver repopulation with hepatocytes generated from human fibroblasts. Nature. 2014;508:93-7 pubmed publisher
Chumarina M, Russ K, Azevedo C, Heuer A, Pihl M, Collin A, et al. Cellular alterations identified in pluripotent stem cell-derived midbrain spheroids generated from a female patient with progressive external ophthalmoplegia and parkinsonism who carries a novel variation (p.Q811R) in the POLG1 gene. Acta Neuropathol Commun. 2019;7:208 pubmed publisher
Zhang L, Xu M, Liu G, Wu R, Meng S, Xiahou K, et al. Generation of induced pluripotent stem cell line (IPTi001-A) from a 62-year old sporadic Alzheimer's disease patient with APOE3 (ε3/ε3) genotype. Stem Cell Res. 2019;41:101589 pubmed publisher
Li Z, Wu J, Wang L, Han W, Yu J, Liu X, et al. Generation of qualified clinical-grade functional hepatocytes from human embryonic stem cells in chemically defined conditions. Cell Death Dis. 2019;10:763 pubmed publisher
Tonon F, Giobbe G, Zambon A, Luni C, Gagliano O, Floreani A, et al. In vitro metabolic zonation through oxygen gradient on a chip. Sci Rep. 2019;9:13557 pubmed publisher
Tiihonen J, Koskuvi M, Storvik M, Hyötyläinen I, Gao Y, Puttonen K, et al. Sex-specific transcriptional and proteomic signatures in schizophrenia. Nat Commun. 2019;10:3933 pubmed publisher
Kolundžić N, Khurana P, Devito L, Jeriha J, Marx J, Hobbs C, et al. Induced pluripotent stem cell line heterozygous for p.R501X mutation in filaggrin: KCLi003-A. Stem Cell Res. 2019;39:101527 pubmed publisher
Minami T, Ishii T, Yasuchika K, Fukumitsu K, Ogiso S, Miyauchi Y, et al. Novel hybrid three-dimensional artificial liver using human induced pluripotent stem cells and a rat decellularized liver scaffold. Regen Ther. 2019;10:127-133 pubmed publisher
Kolundžić N, Khurana P, Hobbs C, Rogar M, Ropret S, Torma H, et al. Induced pluripotent stem cell (iPSC) line from an epidermolysis bullosa simplex patient heterozygous for keratin 5 E475G mutation and with the Dowling Meara phenotype. Stem Cell Res. 2019;37:101424 pubmed publisher
Jing R, Corbett J, Cai J, Beeson G, Beeson C, CHAN S, et al. A Screen Using iPSC-Derived Hepatocytes Reveals NAD+ as a Potential Treatment for mtDNA Depletion Syndrome. Cell Rep. 2018;25:1469-1484.e5 pubmed publisher
Wang Y, Kang C, Yu H, Fen J, Ma X, Lei Y, et al. Establishment of TUSMi004-A, an induced pluripotent stem cell (iPSC) line from a 32-year old Chinese Han patient with Obsessive-Compulsive Disorder (OCD). Stem Cell Res. 2018;32:83-86 pubmed publisher
Oksanen M, Hyötyläinen I, Voutilainen J, Puttonen K, Hämäläinen R, Graff C, et al. Generation of a human induced pluripotent stem cell line (LL008 1.4) from a familial Alzheimer's disease patient carrying a double KM670/671NL (Swedish) mutation in APP gene. Stem Cell Res. 2018;31:181-185 pubmed publisher
Wang Y, Yu H, Chen Y, Li G, Lei Y, Zhao J. Derivation of induced pluripotent stem cells TUSMi006 from an 87-year old Chinese Han Alzheimer's disease patient carrying GRINB and SORL1 mutations. Stem Cell Res. 2018;31:127-130 pubmed publisher
Shaltouki A, Hsieh C, Kim M, Wang X. Alpha-synuclein delays mitophagy and targeting Miro rescues neuron loss in Parkinson's models. Acta Neuropathol. 2018;136:607-620 pubmed publisher
Smutny T, Harjumäki R, Kanninen L, Yliperttula M, Pavek P, Lou Y. A feasibility study of the toxic responses of human induced pluripotent stem cell-derived hepatocytes to phytochemicals. Toxicol In Vitro. 2018;52:94-105 pubmed publisher
Pisal R, Suchanek J, Siller R, Soukup T, Hrebíková H, Bezrouk A, et al. Directed reprogramming of comprehensively characterized dental pulp stem cells extracted from natal tooth. Sci Rep. 2018;8:6168 pubmed publisher
Asumda F, Hatzistergos K, Dykxhoorn D, Jakubski S, Edwards J, Thomas E, et al. Differentiation of hepatocyte-like cells from human pluripotent stem cells using small molecules. Differentiation. 2018;101:16-24 pubmed publisher
Pfeiffer M, Quaranta R, Piccini I, Fell J, Rao J, Röpke A, et al. Cardiogenic programming of human pluripotent stem cells by dose-controlled activation of EOMES. Nat Commun. 2018;9:440 pubmed publisher
Memon B, Karam M, Al Khawaga S, Abdelalim E. Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1. Stem Cell Res Ther. 2018;9:15 pubmed publisher
Han Y, Zhao J, Huang R, Xia M, Wang D. Omics-Based Platform for Studying Chemical Toxicity Using Stem Cells. J Proteome Res. 2018;17:579-589 pubmed publisher
Megaw R, Abu Arafeh H, JUNGNICKEL M, Mellough C, Gurniak C, Witke W, et al. Gelsolin dysfunction causes photoreceptor loss in induced pluripotent cell and animal retinitis pigmentosa models. Nat Commun. 2017;8:271 pubmed publisher
Marczenke M, Piccini I, Mengarelli I, Fell J, Röpke A, Seebohm G, et al. Cardiac Subtype-Specific Modeling of Kv1.5 Ion Channel Deficiency Using Human Pluripotent Stem Cells. Front Physiol. 2017;8:469 pubmed publisher
Bara A, Messana A, Herring A, Hazelbaker D, Eggan K, Barrett L. Generation of a TLE3 heterozygous knockout human embryonic stem cell line using CRISPR-Cas9. Stem Cell Res. 2016;17:441-443 pubmed publisher
Sotthibundhu A, McDonagh K, von Kriegsheim A, Garcia Munoz A, Klawiter A, Thompson K, et al. Rapamycin regulates autophagy and cell adhesion in induced pluripotent stem cells. Stem Cell Res Ther. 2016;7:166 pubmed
Hung S, Van Bergen N, Jackson S, Liang H, Mackey D, Hernández D, et al. Study of mitochondrial respiratory defects on reprogramming to human induced pluripotent stem cells. Aging (Albany NY). 2016;8:945-57 pubmed publisher
Duan S, Yuan G, Liu X, Ren R, Li J, Zhang W, et al. PTEN deficiency reprogrammes human neural stem cells towards a glioblastoma stem cell-like phenotype. Nat Commun. 2015;6:10068 pubmed publisher
Lam A, Li J, Chen A, Reuveny S, Oh S, Birch W. Cationic surface charge combined with either vitronectin or laminin dictates the evolution of human embryonic stem cells/microcarrier aggregates and cell growth in agitated cultures. Stem Cells Dev. 2014;23:1688-703 pubmed publisher
Bershteyn M, Hayashi Y, Desachy G, Hsiao E, Sami S, Tsang K, et al. Cell-autonomous correction of ring chromosomes in human induced pluripotent stem cells. Nature. 2014;507:99-103 pubmed publisher
Lou Y, Kanninen L, Kuisma T, Niklander J, Noon L, Burks D, et al. The use of nanofibrillar cellulose hydrogel as a flexible three-dimensional model to culture human pluripotent stem cells. Stem Cells Dev. 2014;23:380-92 pubmed publisher
Yu Y, Liu H, Ikeda Y, Amiot B, Rinaldo P, Duncan S, et al. Hepatocyte-like cells differentiated from human induced pluripotent stem cells: relevance to cellular therapies. Stem Cell Res. 2012;9:196-207 pubmed publisher
Frank S, Zhang M, Scholer H, Greber B. Small molecule-assisted, line-independent maintenance of human pluripotent stem cells in defined conditions. PLoS ONE. 2012;7:e41958 pubmed publisher
Cao H, Yang P, Pu Y, Sun X, Yin H, Zhang Y, et al. Characterization of bovine induced pluripotent stem cells by lentiviral transduction of reprogramming factor fusion proteins. Int J Biol Sci. 2012;8:498-511 pubmed publisher
Greber B, Coulon P, Zhang M, Moritz S, Frank S, Müller Molina A, et al. FGF signalling inhibits neural induction in human embryonic stem cells. EMBO J. 2011;30:4874-84 pubmed publisher
Ritner C, Wong S, King F, Mihardja S, Liszewski W, Erle D, et al. An engineered cardiac reporter cell line identifies human embryonic stem cell-derived myocardial precursors. PLoS ONE. 2011;6:e16004 pubmed publisher
Fischer Y, Ganic E, Ameri J, Xian X, Johannesson M, Semb H. NANOG reporter cell lines generated by gene targeting in human embryonic stem cells. PLoS ONE. 2010;5: pubmed publisher
Leung H, Chen A, Choo A, Reuveny S, Oh S. Agitation can induce differentiation of human pluripotent stem cells in microcarrier cultures. Tissue Eng Part C Methods. 2011;17:165-72 pubmed publisher
King F, Liszewski W, Ritner C, Bernstein H. High-throughput tracking of pluripotent human embryonic stem cells with dual fluorescence resonance energy transfer molecular beacons. Stem Cells Dev. 2011;20:475-84 pubmed publisher
Gaur M, Ritner C, Sievers R, Pedersen A, Prasad M, Bernstein H, et al. Timed inhibition of p38MAPK directs accelerated differentiation of human embryonic stem cells into cardiomyocytes. Cytotherapy. 2010;12:807-17 pubmed publisher
Armstrong L, Tilgner K, Saretzki G, Atkinson S, Stojkovic M, Moreno R, et al. Human induced pluripotent stem cell lines show stress defense mechanisms and mitochondrial regulation similar to those of human embryonic stem cells. Stem Cells. 2010;28:661-73 pubmed publisher
Sakurai K, Shimoji M, Tahimic C, Aiba K, Kawase E, Hasegawa K, et al. Efficient integration of transgenes into a defined locus in human embryonic stem cells. Nucleic Acids Res. 2010;38:e96 pubmed publisher
Meng G, Liu S, Li X, Krawetz R, Rancourt D. Extracellular matrix isolated from foreskin fibroblasts supports long-term xeno-free human embryonic stem cell culture. Stem Cells Dev. 2010;19:547-56 pubmed publisher
Inada M, Benten D, Cheng K, Joseph B, Berishvili E, Badve S, et al. Stage-specific regulation of adhesion molecule expression segregates epithelial stem/progenitor cells in fetal and adult human livers. Hepatol Int. 2008;2:50-62 pubmed publisher
Xue H, Wu S, Papadeas S, Spusta S, Swistowska A, MacArthur C, et al. A targeted neuroglial reporter line generated by homologous recombination in human embryonic stem cells. Stem Cells. 2009;27:1836-46 pubmed publisher
King F, Ritner C, Liszewski W, Kwan H, Pedersen A, Leavitt A, et al. Subpopulations of human embryonic stem cells with distinct tissue-specific fates can be selected from pluripotent cultures. Stem Cells Dev. 2009;18:1441-50 pubmed publisher
Vaags A, Rosic Kablar S, Gartley C, Zheng Y, Chesney A, Villagómez D, et al. Derivation and characterization of canine embryonic stem cell lines with in vitro and in vivo differentiation potential. Stem Cells. 2009;27:329-40 pubmed publisher
Hasegawa K, Cowan A, Nakatsuji N, Suemori H. Efficient multicistronic expression of a transgene in human embryonic stem cells. Stem Cells. 2007;25:1707-12 pubmed
Clements M, Godfrey A, Crossley J, Wilson S, Takeuchi Y, Boshoff C. Lentiviral manipulation of gene expression in human adult and embryonic stem cells. Tissue Eng. 2006;12:1741-51 pubmed
Cai J, Chen J, Liu Y, Miura T, Luo Y, Loring J, et al. Assessing self-renewal and differentiation in human embryonic stem cell lines. Stem Cells. 2006;24:516-30 pubmed
Brock D, Sutcliffe R. Alpha-fetoprotein in the antenatal diagnosis of anencephaly and spina bifida. Lancet. 1972;2:197-9 pubmed
Abelev G. Alpha-fetoprotein in ontogenesis and its association with malignant tumors. Adv Cancer Res. 1971;14:295-358 pubmed
Yazova A, Goussev A, Poltoranina V, Yakimenko E. Human alpha-fetoprotein epitopes as revealed by monoclonal antibodies. Immunol Lett. 1990;25:325-30 pubmed
product information
Catalog Number :
A8452
Product Name :
Monoclonal Anti-α-Fetoprotein (AFP) antibody produced in mouse
Product Type :
ANTIBODIES - SIAL
Product Group :
Protein & Pathway Technologies
Host Species :
mouse
Clonality :
monoclonal
Conjugation :
unconjugated
Clone Name :
C3
Isotype :
IgG2a
Product Description :
ascites fluid, clone C3
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA