product summary
company name :
MilliporeSigma
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, FLUKA, Sigma-Aldrich, Novabiochem, Guava, Roche Applied Science
product type :
antibody
product name :
Monoclonal Anti-α-Actinin (Sarcomeric) antibody produced in mouse
catalog :
A7811
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
EA-53
reactivity :
Atlantic killifish, jerboas, African green monkey, human, mouse, rat, dogs, chicken, bovine, pigs , zebrafish , Xenopus laevis, domestic rabbit, rhesus macaque
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, other
more info or order :
citations: 778
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; 1:250; fig 5k
Cui M, Atmanli A, Morales M, Tan W, Chen K, Xiao X, et al. Nrf1 promotes heart regeneration and repair by regulating proteostasis and redox balance. Nat Commun. 2021;12:5270 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 1l
Wang J, Zhou C, Khodabukus A, Tran S, Han S, Carlson A, et al. Three-dimensional tissue-engineered human skeletal muscle model of Pompe disease. Commun Biol. 2021;4:524 pubmed publisher
  • immunocytochemistry; African green monkey; 1:1000; loading ...; fig 7b
  • immunocytochemistry; rhesus macaque; 1:1000; loading ...; fig 7b
  • immunocytochemistry; human; 1:1000; loading ...; fig 7b
Stauske M, Rodriguez Polo I, Haas W, Knorr D, Borchert T, Streckfuss Bömeke K, et al. Non-Human Primate iPSC Generation, Cultivation, and Cardiac Differentiation under Chemically Defined Conditions. Cells. 2020;9: pubmed publisher
  • immunocytochemistry; human; loading ...; fig 2b
Taneja N, Neininger A, Burnette D. Coupling to substrate adhesions drives the maturation of muscle stress fibers into myofibrils within cardiomyocytes. Mol Biol Cell. 2020;31:1273-1288 pubmed publisher
  • immunohistochemistry; mouse; 1:800; loading ...; fig 3a
el Azzouzi H, Vilaça A, Feyen D, Gommans W, de Weger R, Doevendans P, et al. Cardiomyocyte Specific Deletion of ADAR1 Causes Severe Cardiac Dysfunction and Increased Lethality. Front Cardiovasc Med. 2020;7:30 pubmed publisher
  • immunocytochemistry; mouse; 1:200; loading ...; fig 5a
Hou M, Han J, Li G, Kwon M, Jiang J, Emani S, et al. Multipotency of mouse trophoblast stem cells. Stem Cell Res Ther. 2020;11:55 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 1d
Guo X, Kolpakov M, Hooshdaran B, Schappell W, Wang T, Eguchi S, et al. Cardiac Expression of Factor X Mediates Cardiac Hypertrophy and Fibrosis in Pressure Overload. JACC Basic Transl Sci. 2020;5:69-83 pubmed publisher
  • immunocytochemistry; human; 1:1000; loading ...; fig 1b
Choi I, Lim H, Cho H, Oh Y, Chou B, Bai H, et al. Transcriptional landscape of myogenesis from human pluripotent stem cells reveals a key role of TWIST1 in maintenance of skeletal muscle progenitors. elife. 2020;9: pubmed publisher
  • immunohistochemistry - frozen section; jerboas; 1:1000; loading ...; fig 5e
Tran M, Tsutsumi R, Erberich J, Chen K, Flores M, Cooper K. Evolutionary loss of foot muscle during development with characteristics of atrophy and no evidence of cell death. elife. 2019;8: pubmed publisher
  • immunohistochemistry; human; 1:200; loading ...; fig 3a
Ahrens Nicklas R, Pappas C, Farman G, Mayfield R, Larrinaga T, Medne L, et al. Disruption of cardiac thin filament assembly arising from a mutation in LMOD2: A novel mechanism of neonatal dilated cardiomyopathy. Sci Adv. 2019;5:eaax2066 pubmed publisher
  • immunohistochemistry; human; loading ...; fig 1d
Birket M, Raibaud S, Lettieri M, Adamson A, Letang V, Cervello P, et al. A Human Stem Cell Model of Fabry Disease Implicates LIMP-2 Accumulation in Cardiomyocyte Pathology. Stem Cell Reports. 2019;13:380-393 pubmed publisher
  • immunocytochemistry; human; 1:100; loading ...; fig 2a
Tsai S, Ghazizadeh Z, Wang H, Ortega F, Badieyan Z, Hsu Z, et al. A Human Embryonic Stem Cell Reporter Line for Monitoring Chemical-induced Cardiotoxicity. Cardiovasc Res. 2019;: pubmed publisher
  • immunocytochemistry; human; 1:500; loading ...; fig s1b
  • immunocytochemistry; African green monkey; 1:500; loading ...; fig s1b
WARD M, Gilad Y. A generally conserved response to hypoxia in iPSC-derived cardiomyocytes from humans and chimpanzees. elife. 2019;8: pubmed publisher
  • immunocytochemistry; human; 1:500; fig s3d
Yap L, Wang J, Moreno Moral A, Chong L, Sun Y, Harmston N, et al. In Vivo Generation of Post-infarct Human Cardiac Muscle by Laminin-Promoted Cardiovascular Progenitors. Cell Rep. 2019;26:3231-3245.e9 pubmed publisher
  • immunocytochemistry; mouse; 1:100; loading ...; fig ev1c
Anderson R, Lagnado A, Maggiorani D, Walaszczyk A, Dookun E, Chapman J, et al. Length-independent telomere damage drives post-mitotic cardiomyocyte senescence. EMBO J. 2019;38: pubmed publisher
  • immunocytochemistry; mouse; 1:250; loading ...; fig 3f
Li Y, Merkel C, Zeng X, Heier J, Cantrell P, Sun M, et al. The N-cadherin interactome in primary cardiomyocytes as defined using quantitative proximity proteomics. J Cell Sci. 2019;132: pubmed publisher
  • immunocytochemistry; rat; 1:200; loading ...; fig 6a
Ly T, Pappas C, Johnson D, Schlecht W, Colpan M, Galkin V, et al. Effects of cardiomyopathy-linked mutations K15N and R21H in tropomyosin on thin-filament regulation and pointed-end dynamics. Mol Biol Cell. 2019;30:268-281 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 2a
Pappas C, Farman G, Mayfield R, Konhilas J, Gregorio C. Cardiac-specific knockout of Lmod2 results in a severe reduction in myofilament force production and rapid cardiac failure. J Mol Cell Cardiol. 2018;122:88-97 pubmed publisher
  • immunocytochemistry; mouse; loading ...; fig 2a
Manalo A, Schroer A, Fenix A, Shancer Z, Coogan J, Brolsma T, et al. Loss of CENP-F Results in Dilated Cardiomyopathy with Severe Disruption of Cardiac Myocyte Architecture. Sci Rep. 2018;8:7546 pubmed publisher
  • immunocytochemistry; mouse; 1:200; loading ...; fig 1a
Yu H, Yuan C, Westenbroek R, Catterall W. The AKAP Cypher/Zasp contributes to β-adrenergic/PKA stimulation of cardiac CaV1.2 calcium channels. J Gen Physiol. 2018;150:883-889 pubmed publisher
  • immunohistochemistry; human; 1:800; loading ...; fig 1b
Anderson D, Kaplan D, Bell K, Koutsis K, Haynes J, Mills R, et al. NKX2-5 regulates human cardiomyogenesis via a HEY2 dependent transcriptional network. Nat Commun. 2018;9:1373 pubmed publisher
  • immunohistochemistry; mouse; 1:400; loading ...; fig s4a
Fujita J, Freire P, Coarfa C, Benham A, Gunaratne P, Schneider M, et al. Ronin Governs Early Heart Development by Controlling Core Gene Expression Programs. Cell Rep. 2017;21:1562-1573 pubmed publisher
  • western blot; human
Al Maqtari T, Hong K, Vajravelu B, Moktar A, Cao P, Moore J, et al. Transcription factor-induced activation of cardiac gene expression in human c-kit+ cardiac progenitor cells. PLoS ONE. 2017;12:e0174242 pubmed publisher
  • immunocytochemistry; mouse; 1:250; loading ...; fig s1b
Keckesova Z, Donaher J, De Cock J, Freinkman E, Lingrell S, Bachovchin D, et al. LACTB is a tumour suppressor that modulates lipid metabolism and cell state. Nature. 2017;543:681-686 pubmed publisher
  • immunocytochemistry; mouse; 1:400; loading ...; fig 1d
Abad M, Hashimoto H, Zhou H, Morales M, Chen B, Bassel Duby R, et al. Notch Inhibition Enhances Cardiac Reprogramming by Increasing MEF2C Transcriptional Activity. Stem Cell Reports. 2017;8:548-560 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100; fig 7f
  • immunocytochemistry; rat; 1:100; fig 2b
Shi J, Bei Y, Kong X, Liu X, Lei Z, Xu T, et al. miR-17-3p Contributes to Exercise-Induced Cardiac Growth and Protects against Myocardial Ischemia-Reperfusion Injury. Theranostics. 2017;7:664-676 pubmed publisher
  • immunohistochemistry; mouse; 1:400; loading ...; fig 2a
Ragni C, Diguet N, Le Garrec J, Novotova M, Resende T, Pop S, et al. Amotl1 mediates sequestration of the Hippo effector Yap1 downstream of Fat4 to restrict heart growth. Nat Commun. 2017;8:14582 pubmed publisher
  • immunohistochemistry; human; loading ...; fig 2a
Lin Y, Zhen Y, Chien K, Lee I, Lin W, Chen M, et al. LIMCH1 regulates nonmuscle myosin-II activity and suppresses cell migration. Mol Biol Cell. 2017;28:1054-1065 pubmed publisher
  • western blot; rat; 1:5000; loading ...; fig 5b
Kovacs A, Kalász J, Pasztor E, Toth A, Papp Z, Dhalla N, et al. Myosin heavy chain and cardiac troponin T damage is associated with impaired myofibrillar ATPase activity contributing to sarcomeric dysfunction in Ca2+-paradox rat hearts. Mol Cell Biochem. 2017;430:57-68 pubmed publisher
  • immunocytochemistry; rat; 1:500; loading ...; fig 5a
Fukuda R, Gunawan F, Beisaw A, Jiménez Amilburu V, Maischein H, Kostin S, et al. Proteolysis regulates cardiomyocyte maturation and tissue integration. Nat Commun. 2017;8:14495 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:50; loading ...; fig st3
Dadson K, Hauck L, Hao Z, Grothe D, Rao V, Mak T, et al. The E3 ligase Mule protects the heart against oxidative stress and mitochondrial dysfunction through Myc-dependent inactivation of Pgc-1α and Pink1. Sci Rep. 2017;7:41490 pubmed publisher
  • immunocytochemistry; mouse; 1:400; fig 1b
Omatsu Kanbe M, Nozuchi N, Nishino Y, Mukaisho K, Sugihara H, Matsuura H. Identification of cardiac progenitors that survive in the ischemic human heart after ventricular myocyte death. Sci Rep. 2017;7:41318 pubmed publisher
  • immunohistochemistry; zebrafish ; loading ...; fig s4b
Ohki Y, Wenninger Weinzierl A, Hruscha A, Asakawa K, Kawakami K, Haass C, et al. Glycine-alanine dipeptide repeat protein contributes to toxicity in a zebrafish model of C9orf72 associated neurodegeneration. Mol Neurodegener. 2017;12:6 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 1d
Christoforou N, Chakraborty S, Kirkton R, Adler A, Addis R, Leong K. Core Transcription Factors, MicroRNAs, and Small Molecules Drive Transdifferentiation of Human Fibroblasts Towards The Cardiac Cell Lineage. Sci Rep. 2017;7:40285 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig s6
Tang J, Shen D, Caranasos T, Wang Z, Vandergriff A, Allen T, et al. Therapeutic microparticles functionalized with biomimetic cardiac stem cell membranes and secretome. Nat Commun. 2017;8:13724 pubmed publisher
  • immunocytochemistry; human; loading ...; fig s2i
Ang Y, Rivas R, Ribeiro A, Srivas R, Rivera J, Stone N, et al. Disease Model of GATA4 Mutation Reveals Transcription Factor Cooperativity in Human Cardiogenesis. Cell. 2016;167:1734-1749.e22 pubmed publisher
  • flow cytometry; human; 1:800; fig 1d
Kempf H, Olmer R, Haase A, Franke A, Bolesani E, Schwanke K, et al. Bulk cell density and Wnt/TGFbeta signalling regulate mesendodermal patterning of human pluripotent stem cells. Nat Commun. 2016;7:13602 pubmed publisher
  • immunocytochemistry; mouse; loading ...; fig 1d
  • western blot; mouse; loading ...; fig 1a
Huang S, Zhou A, Nguyen D, Zhang H, Benz E. Protein 4.1R Influences Myogenin Protein Stability and Skeletal Muscle Differentiation. J Biol Chem. 2016;291:25591-25607 pubmed
  • immunohistochemistry; zebrafish ; 1:100
  • western blot; human; 1:2500; loading ...; fig 4b
O Grady G, Best H, Sztal T, Schartner V, Sanjuan Vazquez M, Donkervoort S, et al. Variants in the Oxidoreductase PYROXD1 Cause Early-Onset Myopathy with Internalized Nuclei and Myofibrillar Disorganization. Am J Hum Genet. 2016;99:1086-1105 pubmed publisher
  • immunocytochemistry; human; 1:250; fig s1
Okata S, Yuasa S, Suzuki T, Ito S, Makita N, Yoshida T, et al. Embryonic type Na+ channel ?-subunit, SCN3B masks the disease phenotype of Brugada syndrome. Sci Rep. 2016;6:34198 pubmed publisher
  • immunocytochemistry; mouse; 1:500; fig 1
Ow J, Palanichamy Kala M, Rao V, Choi M, Bharathy N, Taneja R. G9a inhibits MEF2C activity to control sarcomere assembly. Sci Rep. 2016;6:34163 pubmed publisher
  • immunocytochemistry; human; 1:1000; fig 3
Kim E, Page P, Dellefave Castillo L, McNally E, Wyatt E. Direct reprogramming of urine-derived cells with inducible MyoD for modeling human muscle disease. Skelet Muscle. 2016;6:32 pubmed publisher
  • immunohistochemistry - frozen section; mouse; tbl s5
Rader E, Turk R, Willer T, Beltrán D, Inamori K, Peterson T, et al. Role of dystroglycan in limiting contraction-induced injury to the sarcomeric cytoskeleton of mature skeletal muscle. Proc Natl Acad Sci U S A. 2016;113:10992-7 pubmed publisher
  • immunohistochemistry - paraffin section; zebrafish ; 1:200; loading ...; fig 1j
Cheng F, Miao L, Wu Q, Gong X, Xiong J, Zhang J. Vinculin b deficiency causes epicardial hyperplasia and coronary vessel disorganization in zebrafish. Development. 2016;143:3522-3531 pubmed
  • western blot; mouse; fig s3
Kudová J, Prochazkova J, Vašíček O, Perecko T, Sedláčková M, Pesl M, et al. HIF-1alpha Deficiency Attenuates the Cardiomyogenesis of Mouse Embryonic Stem Cells. PLoS ONE. 2016;11:e0158358 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 3a
Boogerd C, Aneas I, Sakabe N, Dirschinger R, Cheng Q, Zhou B, et al. Probing chromatin landscape reveals roles of endocardial TBX20 in septation. J Clin Invest. 2016;126:3023-35 pubmed publisher
  • immunohistochemistry; mouse; 1:200; fig 1B
Kanda M, Nagai T, Takahashi T, Liu M, Kondou N, Naito A, et al. Leukemia Inhibitory Factor Enhances Endogenous Cardiomyocyte Regeneration after Myocardial Infarction. PLoS ONE. 2016;11:e0156562 pubmed publisher
  • western blot; mouse; fig 1
Zhang L, Lu X, Gui L, Wu Y, Sims S, Wang G, et al. Inhibition of Rac1 reduces store overload-induced calcium release and protects against ventricular arrhythmia. J Cell Mol Med. 2016;20:1513-22 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:800; fig 2
  • flow cytometry; human; 1:800; fig s1
Mannhardt I, Breckwoldt K, Letuffe Brenière D, Schaaf S, Schulz H, Neuber C, et al. Human Engineered Heart Tissue: Analysis of Contractile Force. Stem Cell Reports. 2016;7:29-42 pubmed publisher
  • immunocytochemistry; mouse; 1:1000; fig 1b
Shi H, Drummond C, Fan X, Haller S, Liu J, Malhotra D, et al. Hiding inside? Intracellular expression of non-glycosylated c-kit protein in cardiac progenitor cells. Stem Cell Res. 2016;16:795-806 pubmed publisher
  • western blot; human; fig 2
Winter L, Türk M, Harter P, Mittelbronn M, Kornblum C, Norwood F, et al. Downstream effects of plectin mutations in epidermolysis bullosa simplex with muscular dystrophy. Acta Neuropathol Commun. 2016;4:44 pubmed publisher
  • immunocytochemistry; human; fig 4k
Suchanek J, Suchánková Kleplová T, Rehacek V, Browne K, Soukup T. Proliferative Capacity and Phenotypical Alteration of Multipotent Ecto-Mesenchymal Stem Cells from Human Exfoliated Deciduous Teeth Cultured in Xenogeneic and Allogeneic Media. Folia Biol (Praha). 2016;62:1-14 pubmed
  • immunohistochemistry; zebrafish ; 1:100; fig 7
Ruparelia A, Oorschot V, Ramm G, Bryson Richardson R. FLNC myofibrillar myopathy results from impaired autophagy and protein insufficiency. Hum Mol Genet. 2016;25:2131-2142 pubmed
  • immunocytochemistry; rat; 1:200; fig 5
Cannavo A, Liccardo D, Eguchi A, Elliott K, Traynham C, Ibetti J, et al. Myocardial pathology induced by aldosterone is dependent on non-canonical activities of G protein-coupled receptor kinases. Nat Commun. 2016;7:10877 pubmed publisher
  • immunohistochemistry; mouse; 1:1000; loading ...; fig 1e
Yu W, Huang X, Tian X, Zhang H, He L, Wang Y, et al. GATA4 regulates Fgf16 to promote heart repair after injury. Development. 2016;143:936-49 pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig 5
Passer D, van de Vrugt A, Atmanli A, Domian I. Atypical Protein Kinase C-Dependent Polarized Cell Division Is Required for Myocardial Trabeculation. Cell Rep. 2016;14:1662-1672 pubmed publisher
  • immunocytochemistry; mouse; loading ...; fig s1a
Zhang W, St Clair D, Butterfield A, Vore M. Loss of Mrp1 Potentiates Doxorubicin-Induced Cytotoxicity in Neonatal Mouse Cardiomyocytes and Cardiac Fibroblasts. Toxicol Sci. 2016;151:44-56 pubmed publisher
  • immunocytochemistry; human; 1:500; fig 1
Eng G, Lee B, Protas L, Gagliardi M, Brown K, Kass R, et al. Autonomous beating rate adaptation in human stem cell-derived cardiomyocytes. Nat Commun. 2016;7:10312 pubmed publisher
  • western blot; human; fig 9
Maggio I, Stefanucci L, Janssen J, Liu J, Chen X, Mouly V, et al. Selection-free gene repair after adenoviral vector transduction of designer nucleases: rescue of dystrophin synthesis in DMD muscle cell populations. Nucleic Acids Res. 2016;44:1449-70 pubmed publisher
  • immunocytochemistry; mouse; 1:100; fig 4
Ban K, Wile B, Cho K, Kim S, Song M, Kim S, et al. Non-genetic Purification of Ventricular Cardiomyocytes from Differentiating Embryonic Stem Cells through Molecular Beacons Targeting IRX-4. Stem Cell Reports. 2015;5:1239-1249 pubmed publisher
  • immunohistochemistry - frozen section; zebrafish ; fig 5
Furlan S, Mosole S, Murgia M, Nagaraj N, Argenton F, Volpe P, et al. Calsequestrins in skeletal and cardiac muscle from adult Danio rerio. J Muscle Res Cell Motil. 2016;37:27-39 pubmed publisher
  • immunohistochemistry; mouse; 1:100-1:200; fig 4
Kraut B, Maier H, Kókai E, Fiedler K, Boettger T, Illing A, et al. Cardiac-Specific Activation of IKK2 Leads to Defects in Heart Development and Embryonic Lethality. PLoS ONE. 2015;10:e0141591 pubmed publisher
  • other; mouse; 1:800; loading ...; fig 2a
Saito Y, Nakamura K, Yoshida M, Sugiyama H, Ohe T, Kurokawa J, et al. Enhancement of Spontaneous Activity by HCN4 Overexpression in Mouse Embryonic Stem Cell-Derived Cardiomyocytes - A Possible Biological Pacemaker. PLoS ONE. 2015;10:e0138193 pubmed publisher
  • immunocytochemistry; mouse; 1:300; fig 2
Zhao Y, Londono P, Cao Y, Sharpe E, Proenza C, O Rourke R, et al. High-efficiency reprogramming of fibroblasts into cardiomyocytes requires suppression of pro-fibrotic signalling. Nat Commun. 2015;6:8243 pubmed publisher
  • western blot; human; 1:200
Jeong M, Kim S, Kang H, Park K, Park W, Yang S, et al. Cucurbitacin I Attenuates Cardiomyocyte Hypertrophy via Inhibition of Connective Tissue Growth Factor (CCN2) and TGF- β/Smads Signalings. PLoS ONE. 2015;10:e0136236 pubmed publisher
  • immunocytochemistry; mouse
Fu Y, Huang C, Xu X, Gu H, Ye Y, Jiang C, et al. Direct reprogramming of mouse fibroblasts into cardiomyocytes with chemical cocktails. Cell Res. 2015;25:1013-24 pubmed publisher
  • western blot; human; fig 4b
Birket M, Ribeiro M, Verkerk A, Ward D, Leitoguinho A, Den Hartogh S, et al. Expansion and patterning of cardiovascular progenitors derived from human pluripotent stem cells. Nat Biotechnol. 2015;33:970-9 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:5000; fig 7
Li F, Buck D, De Winter J, Kolb J, Meng H, Birch C, et al. Nebulin deficiency in adult muscle causes sarcomere defects and muscle-type-dependent changes in trophicity: novel insights in nemaline myopathy. Hum Mol Genet. 2015;24:5219-33 pubmed publisher
  • immunocytochemistry; mouse
Yang J, Kaur K, Ong L, Eisenberg C, Eisenberg L. Inhibition of G9a Histone Methyltransferase Converts Bone Marrow Mesenchymal Stem Cells to Cardiac Competent Progenitors. Stem Cells Int. 2015;2015:270428 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:200
Wei K, Díaz Trelles R, Liu Q, Diez Cuñado M, Scimia M, Cai W, et al. Developmental origin of age-related coronary artery disease. Cardiovasc Res. 2015;107:287-94 pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig 3
Tian L, Ding S, You Y, Li T, Liu Y, Wu X, et al. Leiomodin-3-deficient mice display nemaline myopathy with fast-myofiber atrophy. Dis Model Mech. 2015;8:635-41 pubmed publisher
  • immunocytochemistry; human; 1:100; fig 2
Zatti S, Martewicz S, Serena E, Uno N, Giobbe G, Kazuki Y, et al. Complete restoration of multiple dystrophin isoforms in genetically corrected Duchenne muscular dystrophy patient-derived cardiomyocytes. Mol Ther Methods Clin Dev. 2014;1:1 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; fig 4
Liang X, Ding Y, Zhang Y, Chai Y, He J, Chiu S, et al. Activation of NRG1-ERBB4 signaling potentiates mesenchymal stem cell-mediated myocardial repairs following myocardial infarction. Cell Death Dis. 2015;6:e1765 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:500
  • western blot; mouse
Mastrototaro G, Liang X, Li X, Carullo P, Piroddi N, Tesi C, et al. Nebulette knockout mice have normal cardiac function, but show Z-line widening and up-regulation of cardiac stress markers. Cardiovasc Res. 2015;107:216-25 pubmed publisher
  • western blot; human
Kalinowska M, Chávez A, Lutzu S, Castillo P, Bukauskas F, Francesconi A. Actinin-4 Governs Dendritic Spine Dynamics and Promotes Their Remodeling by Metabotropic Glutamate Receptors. J Biol Chem. 2015;290:15909-20 pubmed publisher
  • immunohistochemistry - frozen section; zebrafish ; 1:100
Sztal T, Zhao M, Williams C, Oorschot V, Parslow A, Giousoh A, et al. Zebrafish models for nemaline myopathy reveal a spectrum of nemaline bodies contributing to reduced muscle function. Acta Neuropathol. 2015;130:389-406 pubmed publisher
  • western blot; rat
Sin Y, Martin T, Wills L, Currie S, Baillie G. Small heat shock protein 20 (Hsp20) facilitates nuclear import of protein kinase D 1 (PKD1) during cardiac hypertrophy. Cell Commun Signal. 2015;13:16 pubmed publisher
  • flow cytometry; human; fig 1
Jha R, Xu R, Xu C. Efficient differentiation of cardiomyocytes from human pluripotent stem cells with growth factors. Methods Mol Biol. 2015;1299:115-31 pubmed publisher
  • immunocytochemistry; human; 1:800; fig 6
Zhang M, Schulte J, Heinick A, Piccini I, Rao J, Quaranta R, et al. Universal cardiac induction of human pluripotent stem cells in two and three-dimensional formats: implications for in vitro maturation. Stem Cells. 2015;33:1456-69 pubmed publisher
  • immunocytochemistry; mouse; 1:400; fig 1e
Choi S, Lee H, Choi J, Kim J, Park C, Joo H, et al. Cyclosporin A induces cardiac differentiation but inhibits hemato-endothelial differentiation of P19 cells. PLoS ONE. 2015;10:e0117410 pubmed publisher
  • immunocytochemistry; human; 1:800
van den Berg C, Elliott D, Braam S, Mummery C, Davis R. Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes Under Defined Conditions. Methods Mol Biol. 2016;1353:163-80 pubmed publisher
  • immunocytochemistry; mouse; 1:500
Reuter S, Soonpaa M, Firulli A, Chang A, Field L. Recombinant neuregulin 1 does not activate cardiomyocyte DNA synthesis in normal or infarcted adult mice. PLoS ONE. 2014;9:e115871 pubmed publisher
  • immunohistochemistry; rat; 1:200
Hou Y, Jayasinghe I, Crossman D, Baddeley D, Soeller C. Nanoscale analysis of ryanodine receptor clusters in dyadic couplings of rat cardiac myocytes. J Mol Cell Cardiol. 2015;80:45-55 pubmed publisher
  • immunohistochemistry; human; 1:200
Swager S, Delfín D, Rastogi N, Wang H, Canan B, Fedorov V, et al. Claudin-5 levels are reduced from multiple cell types in human failing hearts and are associated with mislocalization of ephrin-B1. Cardiovasc Pathol. 2015;24:160-167 pubmed publisher
  • immunocytochemistry; mouse; 1:400; fig 3
Nam Y, Lubczyk C, Bhakta M, Zang T, Fernandez Perez A, McAnally J, et al. Induction of diverse cardiac cell types by reprogramming fibroblasts with cardiac transcription factors. Development. 2014;141:4267-78 pubmed publisher
  • immunohistochemistry; human; 1:200; fig 4
Tchao J, Han L, Lin B, Yang L, Tobita K. Combined biophysical and soluble factor modulation induces cardiomyocyte differentiation from human muscle derived stem cells. Sci Rep. 2014;4:6614 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100
He L, Tian X, Zhang H, Hu T, Huang X, Zhang L, et al. BAF200 is required for heart morphogenesis and coronary artery development. PLoS ONE. 2014;9:e109493 pubmed publisher
  • immunohistochemistry; zebrafish ; 1:100
Ruparelia A, Oorschot V, Vaz R, Ramm G, Bryson Richardson R. Zebrafish models of BAG3 myofibrillar myopathy suggest a toxic gain of function leading to BAG3 insufficiency. Acta Neuropathol. 2014;128:821-33 pubmed publisher
  • immunocytochemistry; mouse; fig 4
White J, Barro M, Makarenkova H, Sanger J, Sanger J. Localization of sarcomeric proteins during myofibril assembly in cultured mouse primary skeletal myotubes. Anat Rec (Hoboken). 2014;297:1571-84 pubmed publisher
  • western blot; mouse; 1:5000; fig 5
Wein N, Vulin A, Falzarano M, Szigyarto C, Maiti B, Findlay A, et al. Translation from a DMD exon 5 IRES results in a functional dystrophin isoform that attenuates dystrophinopathy in humans and mice. Nat Med. 2014;20:992-1000 pubmed publisher
  • immunocytochemistry; rat
Bingen B, Engels M, Schalij M, Jangsangthong W, Neshati Z, Feola I, et al. Light-induced termination of spiral wave arrhythmias by optogenetic engineering of atrial cardiomyocytes. Cardiovasc Res. 2014;104:194-205 pubmed publisher
  • immunocytochemistry; mouse; 1:200
Martinez Fernandez A, Nelson T, Reyes S, Alekseev A, Secreto F, Perez Terzic C, et al. iPS cell-derived cardiogenicity is hindered by sustained integration of reprogramming transgenes. Circ Cardiovasc Genet. 2014;7:667-76 pubmed publisher
  • immunohistochemistry - frozen section; mouse
Can T, Faas L, Ashford D, Dowle A, Thomas J, O Toole P, et al. Proteomic analysis of laser capture microscopy purified myotendinous junction regions from muscle sections. Proteome Sci. 2014;12:25 pubmed publisher
  • immunohistochemistry; human
Janbaz A, Lindström M, Liu J, Pedrosa Domellöf F. Intermediate filaments in the human extraocular muscles. Invest Ophthalmol Vis Sci. 2014;55:5151-9 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 4e
Kudo Sakamoto Y, Akazawa H, Ito K, Takano J, Yano M, Yabumoto C, et al. Calpain-dependent cleavage of N-cadherin is involved in the progression of post-myocardial infarction remodeling. J Biol Chem. 2014;289:19408-19 pubmed publisher
  • immunohistochemistry - paraffin section; mouse
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product information
Product No. :
A7811
Product Name :
Monoclonal Anti-α-Actinin (Sarcomeric) antibody produced in mouse
Host :
mouse
Conjugate :
unconjugated
Antibody Type :
monoclonal
Clone Number (if monoclonal) :
EA-53
Applications/Description :
Suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:800 using human skeletal and cardiac muscle; western blot: 1:2,500 using rat leg muscle; indirect immunofluorescence using human and animal cultured muscle cells; indirect ELISA:
Species Reactivity :
sheep, rabbit, lizard, feline, snake, canine, fish, human, mouse, goat, chicken, rat, frog, bovine, pig, hamster
Isotype :
IgG1
Storage :
Store at -20°C
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA