product summary
company name :
MilliporeSigma
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, FLUKA, Sigma-Aldrich, Novabiochem, Guava, Roche Applied Science
product type :
antibody
product name :
Monoclonal Anti-β-Actin antibody produced in mouse
catalog :
A5316
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
AC-74
reactivity :
African green monkey, Japanese rice fish, human, mouse, rat, dogs, chicken, bovine, Xenopus laevis
application :
western blot, immunocytochemistry, immunoprecipitation
more info or order :
citations: 2850
Published Application/Species/Sample/DilutionReference
  • western blot; human; loading ...; fig 4a
Sil S, Singh S, Chemparathy D, Chivero E, Gordon L, Buch S. Astrocytes & Astrocyte derived Extracellular Vesicles in Morphine Induced Amyloidopathy: Implications for Cognitive Deficits in Opiate Abusers. Aging Dis. 2021;12:1389-1408 pubmed publisher
  • western blot; human; 1:2000; loading ...; fig 7d
De Groof T, Bergkamp N, Heukers R, Giap T, Bebelman M, Goeij de Haas R, et al. Selective targeting of ligand-dependent and -independent signaling by GPCR conformation-specific anti-US28 intrabodies. Nat Commun. 2021;12:4357 pubmed publisher
  • western blot; mouse; 1:50,000; loading ...; fig 5b
Gaja Capdevila N, Hernández N, Zamanillo D, Vela J, Merlos M, Navarro X, et al. Neuroprotective Effects of Sigma 1 Receptor Ligands on Motoneuron Death after Spinal Root Injury in Mice. Int J Mol Sci. 2021;22: pubmed publisher
  • western blot; human; loading ...; fig 1d
Padmanabhan N, Kyon H, Boot A, Lim K, Srivastava S, Chen S, et al. Highly recurrent CBS epimutations in gastric cancer CpG island methylator phenotypes and inflammation. Genome Biol. 2021;22:167 pubmed publisher
  • western blot; human; loading ...; fig 2c
Nakajima W, Miyazaki K, Asano Y, Kubota S, Tanaka N. Krüppel-Like Factor 4 and Its Activator APTO-253 Induce NOXA-Mediated, p53-Independent Apoptosis in Triple-Negative Breast Cancer Cells. Genes (Basel). 2021;12: pubmed publisher
  • western blot; human; 1:10,000; loading ...; fig 3a
Sato K, Hikita H, Myojin Y, Fukumoto K, Murai K, Sakane S, et al. Hyperglycemia enhances pancreatic cancer progression accompanied by elevations in phosphorylated STAT3 and MYC levels. PLoS ONE. 2020;15:e0235573 pubmed publisher
  • western blot; mouse; 1:2000; loading ...; fig 3h
Sato T, Kataoka K, Ito Y, Yokoyama S, Inui M, Mori M, et al. Lin28a/let-7 pathway modulates the Hox code via Polycomb regulation during axial patterning in vertebrates. elife. 2020;9: pubmed publisher
  • western blot; mouse; 1:5000; loading ...; fig 4d
Alajati A, D Ambrosio M, Troiani M, Mosole S, Pellegrini L, Chen J, et al. CDCP1 overexpression drives prostate cancer progression and can be targeted in vivo. J Clin Invest. 2020;130:2435-2450 pubmed publisher
  • western blot; human; 1:5000; fig 3d
Rana S, Espinosa Díez C, Ruhl R, Chatterjee N, Hudson C, Fraile Bethencourt E, et al. Differential regulation of microRNA-15a by radiation affects angiogenesis and tumor growth via modulation of acid sphingomyelinase. Sci Rep. 2020;10:5581 pubmed publisher
  • western blot; mouse; 1:10,000; loading ...; fig 4d
Eom T, Han S, Kim J, Blundon J, Wang Y, Yu J, et al. Schizophrenia-related microdeletion causes defective ciliary motility and brain ventricle enlargement via microRNA-dependent mechanisms in mice. Nat Commun. 2020;11:912 pubmed publisher
  • western blot; mouse; loading ...; fig 6a
Zhai Y, Zhu Y, Liu J, Xie K, Yu J, Yu L, et al. Dexmedetomidine Post-Conditioning Alleviates Cerebral Ischemia-Reperfusion Injury in Rats by Inhibiting High Mobility Group Protein B1 Group (HMGB1)/Toll-Like Receptor 4 (TLR4)/Nuclear Factor kappa B (NF-κB) Signaling Pathway. Med Sci Monit. 2020;26:e918617 pubmed publisher
  • western blot; mouse; loading ...; fig 1f
Choi W, Kim H, Kim M, Cinar R, Yi H, Eun H, et al. Glutamate Signaling in Hepatic Stellate Cells Drives Alcoholic Steatosis. Cell Metab. 2019;30:877-889.e7 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 2d
Gao X, Chen H, Liu J, Shen S, Wang Q, Clement T, et al. The REGγ-Proteasome Regulates Spermatogenesis Partially by P53-PLZF Signaling. Stem Cell Reports. 2019;13:559-571 pubmed publisher
  • western blot; human; 1:10,000; loading ...; fig 3
Wohlrab C, Kuiper C, Vissers M, Phillips E, Robinson B, Dachs G. Ascorbate modulates the hypoxic pathway by increasing intracellular activity of the HIF hydroxylases in renal cell carcinoma cells. Hypoxia (Auckl). 2019;7:17-31 pubmed publisher
  • western blot; human; 1:5000; loading ...; fig s3e
Wu S, Fatkhutdinov N, Rosin L, Luppino J, Iwasaki O, Tanizawa H, et al. ARID1A spatially partitions interphase chromosomes. Sci Adv. 2019;5:eaaw5294 pubmed publisher
  • western blot; human; 1:5000; loading ...; fig 5d
Liu X, Zhou Z, Li W, Zhang S, Li J, Zhou M, et al. Heparanase Promotes Tumor Growth and Liver Metastasis of Colorectal Cancer Cells by Activating the p38/MMP1 Axis. Front Oncol. 2019;9:216 pubmed publisher
  • western blot; human; 1:200; loading ...; fig 2d
Li Y, Wang L, Rivera Serrano E, Chen X, Lemon S. TNRC6 proteins modulate hepatitis C virus replication by spatially regulating the binding of miR-122/Ago2 complexes to viral RNA. Nucleic Acids Res. 2019;47:6411-6424 pubmed publisher
  • western blot; human; fig 5b
Zhang Q, Higginbotham J, Jeppesen D, Yang Y, Li W, McKinley E, et al. Transfer of Functional Cargo in Exomeres. Cell Rep. 2019;27:940-954.e6 pubmed publisher
  • western blot; mouse; 1:2500; loading ...; fig 1a
Upadhyay A, Hosseinibarkooie S, Schneider S, Kaczmarek A, Torres Benito L, Mendoza Ferreira N, et al. Neurocalcin Delta Knockout Impairs Adult Neurogenesis Whereas Half Reduction Is Not Pathological. Front Mol Neurosci. 2019;12:19 pubmed publisher
  • western blot; human; 1:5000; loading ...; fig 3f
Niu F, Liao K, Hu G, Sil S, Callen S, Guo M, et al. Cocaine-induced release of CXCL10 from pericytes regulates monocyte transmigration into the CNS. J Cell Biol. 2019;218:700-721 pubmed publisher
  • western blot; mouse; loading ...; fig 4b
MacFarlane E, Parker S, Shin J, Kang B, Ziegler S, Creamer T, et al. Lineage-specific events underlie aortic root aneurysm pathogenesis in Loeys-Dietz syndrome. J Clin Invest. 2019;129:659-675 pubmed publisher
  • western blot; human; loading ...; fig 1d
Jachimowicz R, Beleggia F, Isensee J, Velpula B, Goergens J, Bustos M, et al. UBQLN4 Represses Homologous Recombination and Is Overexpressed in Aggressive Tumors. Cell. 2019;176:505-519.e22 pubmed publisher
  • western blot; human; loading ...; fig 5a
Song J, Zhang X, Liao Z, Liang H, Chu L, Dong W, et al. 14-3-3ζ inhibits heme oxygenase-1 (HO-1) degradation and promotes hepatocellular carcinoma proliferation: involvement of STAT3 signaling. J Exp Clin Cancer Res. 2019;38:3 pubmed publisher
  • western blot; Xenopus laevis; 1:1000; fig 1a
Schrank B, Aparicio T, Li Y, Chang W, Chait B, Gundersen G, et al. Nuclear ARP2/3 drives DNA break clustering for homology-directed repair. Nature. 2018;559:61-66 pubmed publisher
  • western blot; mouse; loading ...; fig 1b
Iseka F, Goetz B, Mushtaq I, An W, Cypher L, Bielecki T, et al. Role of the EHD Family of Endocytic Recycling Regulators for TCR Recycling and T Cell Function. J Immunol. 2018;200:483-499 pubmed publisher
  • immunocytochemistry; mouse; loading ...; fig 4b
Chen J, Zhong M, Guo H, Davidson D, Mishel S, Lu Y, et al. SLAMF7 is critical for phagocytosis of haematopoietic tumour cells via Mac-1 integrin. Nature. 2017;544:493-497 pubmed publisher
  • western blot; mouse; fig 1
Fogarty L, Song B, Suppiah Y, Hasan S, Martin H, Hogan S, et al. Bcl-xL dependency coincides with the onset of neurogenesis in the developing mammalian spinal cord. Mol Cell Neurosci. 2016;77:34-46 pubmed publisher
  • western blot; human; fig 1
Chai Y, Sierecki E, Tomatis V, Gormal R, Giles N, Morrow I, et al. Munc18-1 is a molecular chaperone for α-synuclein, controlling its self-replicating aggregation. J Cell Biol. 2016;214:705-18 pubmed publisher
  • western blot; human; fig 1
Sato N, Takasaka N, Yoshida M, Tsubouchi K, Minagawa S, Araya J, et al. Metformin attenuates lung fibrosis development via NOX4 suppression. Respir Res. 2016;17:107 pubmed publisher
  • western blot; human; fig 3
Valencia Gattas M, Conner G, Fregien N. Gefitinib, an EGFR Tyrosine Kinase inhibitor, Prevents Smoke-Mediated Ciliated Airway Epithelial Cell Loss and Promotes Their Recovery. PLoS ONE. 2016;11:e0160216 pubmed publisher
  • western blot; mouse; 1:1000; fig 3
Nakamichi R, Ito Y, Inui M, Onizuka N, Kayama T, Kataoka K, et al. Mohawk promotes the maintenance and regeneration of the outer annulus fibrosus of intervertebral discs. Nat Commun. 2016;7:12503 pubmed publisher
  • western blot; human; fig 2
Kariya Y, Tatsuta T, Sugawara S, Kariya Y, Nitta K, Hosono M. RNase activity of sialic acid-binding lectin from bullfrog eggs drives antitumor effect via the activation of p38 MAPK to caspase-3/7 signaling pathway in human breast cancer cells. Int J Oncol. 2016;49:1334-42 pubmed publisher
  • immunocytochemistry; human; 1:100; fig 4
Busse B, Bezrukov L, Blank P, Zimmerberg J. Resin embedded multicycle imaging (REMI): a tool to evaluate protein domains. Sci Rep. 2016;6:30284 pubmed publisher
  • western blot; Japanese rice fish; fig 3
Yuan Y, Wang Y, Liu Q, Zhu F, Hong Y. Singapore grouper iridovirus protein VP088 is essential for viral infectivity. Sci Rep. 2016;6:31170 pubmed publisher
  • western blot; human; 1:1000; fig 3h
Kaukonen R, Mai A, Georgiadou M, Saari M, De Franceschi N, Betz T, et al. Normal stroma suppresses cancer cell proliferation via mechanosensitive regulation of JMJD1a-mediated transcription. Nat Commun. 2016;7:12237 pubmed publisher
  • western blot; mouse; 1:10,000; fig 3
Lai M, Gonzalez Martin A, Cooper A, Oda H, Jin H, Shepherd J, et al. Regulation of B-cell development and tolerance by different members of the miR-17∼92 family microRNAs. Nat Commun. 2016;7:12207 pubmed publisher
  • western blot; human; fig 1
Liu X, Ehmed E, Li B, Dou J, Qiao X, Jiang W, et al. Breast cancer metastasis suppressor 1 modulates SIRT1-dependent p53 deacetylation through interacting with DBC1. Am J Cancer Res. 2016;6:1441-9 pubmed
  • western blot; mouse; 1:2000; fig 1
Jang H, Lee G, Selby C, Lee G, Jeon Y, Lee J, et al. SREBP1c-CRY1 signalling represses hepatic glucose production by promoting FOXO1 degradation during refeeding. Nat Commun. 2016;7:12180 pubmed publisher
  • western blot; mouse; fig 2
Hogg S, Newbold A, Vervoort S, Cluse L, Martin B, Gregory G, et al. BET Inhibition Induces Apoptosis in Aggressive B-Cell Lymphoma via Epigenetic Regulation of BCL-2 Family Members. Mol Cancer Ther. 2016;15:2030-41 pubmed publisher
  • western blot; human; fig 3
Seo H, Lee C, Lee J, Lim S, Choi E, Park J, et al. The role of nuclear factor of activated T cells during phorbol myristate acetate-induced cardiac differentiation of mesenchymal stem cells. Stem Cell Res Ther. 2016;7:90 pubmed publisher
  • western blot; human; 1:2000; fig s3
Kloten V, Schlensog M, Eschenbruch J, Gasthaus J, Tiedemann J, Mijnes J, et al. Abundant NDRG2 Expression Is Associated with Aggressiveness and Unfavorable Patients' Outcome in Basal-Like Breast Cancer. PLoS ONE. 2016;11:e0159073 pubmed publisher
  • western blot; mouse; fig s2
Xiao L, Feng Q, Zhang Z, Wang F, Lydon J, Ittmann M, et al. The essential role of GATA transcription factors in adult murine prostate. Oncotarget. 2016;7:47891-47903 pubmed publisher
  • western blot; human; fig 1
Gayatri S, Cowles M, Vemulapalli V, Cheng D, Sun Z, Bedford M. Using oriented peptide array libraries to evaluate methylarginine-specific antibodies and arginine methyltransferase substrate motifs. Sci Rep. 2016;6:28718 pubmed publisher
  • western blot; mouse; fig 1
Fernández Nogales M, Santos Galindo M, Merchán Rubira J, Hoozemans J, Rábano A, Ferrer I, et al. Tau-positive nuclear indentations in P301S tauopathy mice. Brain Pathol. 2017;27:314-322 pubmed publisher
  • western blot; human; 1:10,000; fig 3
Timms R, Menzies S, Tchasovnikarova I, Christensen L, Williamson J, Antrobus R, et al. Genetic dissection of mammalian ERAD through comparative haploid and CRISPR forward genetic screens. Nat Commun. 2016;7:11786 pubmed publisher
  • western blot; human; fig 5
Lo Dico A, Costa V, Martelli C, Diceglie C, Rajata F, Rizzo A, et al. MiR675-5p Acts on HIF-1? to Sustain Hypoxic Responses: A New Therapeutic Strategy for Glioma. Theranostics. 2016;6:1105-18 pubmed publisher
  • western blot; human; 1:5000; fig 1
Mukhopadhyay A, Sehgal L, Bose A, Gulvady A, Senapati P, Thorat R, et al. 14-3-3? Prevents Centrosome Amplification and Neoplastic Progression. Sci Rep. 2016;6:26580 pubmed publisher
  • western blot; mouse; fig 3
Minakawa Y, Atsumi Y, Shinohara A, Murakami Y, Yoshioka K. Gamma-irradiated quiescent cells repair directly induced double-strand breaks but accumulate persistent double-strand breaks during subsequent DNA replication. Genes Cells. 2016;21:789-97 pubmed publisher
  • western blot; mouse; fig 1
Zeng L, Cai C, Li S, Wang W, Li Y, Chen J, et al. Essential Roles of Cyclin Y-Like 1 and Cyclin Y in Dividing Wnt-Responsive Mammary Stem/Progenitor Cells. PLoS Genet. 2016;12:e1006055 pubmed publisher
  • western blot; mouse; fig 4
de Jong P, Taniguchi K, Harris A, Bertin S, Takahashi N, Duong J, et al. ERK5 signalling rescues intestinal epithelial turnover and tumour cell proliferation upon ERK1/2 abrogation. Nat Commun. 2016;7:11551 pubmed publisher
  • western blot; mouse; 1:10,000; fig s9
Devaraju P, Yu J, Eddins D, Mellado Lagarde M, Earls L, Westmoreland J, et al. Haploinsufficiency of the 22q11.2 microdeletion gene Mrpl40 disrupts short-term synaptic plasticity and working memory through dysregulation of mitochondrial calcium. Mol Psychiatry. 2017;22:1313-1326 pubmed publisher
  • western blot; mouse; fig 4
Benjamin J, Van Der Meer R, Im A, Plosa E, Zaynagetdinov R, Burman A, et al. Epithelial-Derived Inflammation Disrupts Elastin Assembly and Alters Saccular Stage Lung Development. Am J Pathol. 2016;186:1786-1800 pubmed publisher
  • western blot; human; fig s2
Hamam D, Abdouh M, Gao Z, Arena V, Arena M, Arena G. Transfer of malignant trait to BRCA1 deficient human fibroblasts following exposure to serum of cancer patients. J Exp Clin Cancer Res. 2016;35:80 pubmed publisher
  • western blot; human; fig 1
Tenorio M, Ross B, Luchsinger C, Rivera Dictter A, Arriagada C, Acuña D, et al. Distinct Biochemical Pools of Golgi Phosphoprotein 3 in the Human Breast Cancer Cell Lines MCF7 and MDA-MB-231. PLoS ONE. 2016;11:e0154719 pubmed publisher
  • western blot; human
Matias A, Manieri T, Cerchiaro G. Zinc Chelation Mediates the Lysosomal Disruption without Intracellular ROS Generation. Oxid Med Cell Longev. 2016;2016:6724585 pubmed publisher
  • western blot; human; fig 2
Scott A, Wilkinson A, Wilkinson J. Basal metabolic state governs AIF-dependent growth support in pancreatic cancer cells. BMC Cancer. 2016;16:286 pubmed publisher
  • western blot; human; fig 1
Leung C, Mak W, Kai A, Chan K, Lee T, Ng I, et al. Sox9 confers stemness properties in hepatocellular carcinoma through Frizzled-7 mediated Wnt/?-catenin signaling. Oncotarget. 2016;7:29371-86 pubmed publisher
  • western blot; human; fig 1
Ren H, Elgner F, Jiang B, Himmelsbach K, Medvedev R, Ploen D, et al. The Autophagosomal SNARE Protein Syntaxin 17 Is an Essential Factor for the Hepatitis C Virus Life Cycle. J Virol. 2016;90:5989-6000 pubmed publisher
  • western blot; human; 1:5000; fig s6
  • western blot; mouse; 1:5000; fig s4
Haemmerle M, Bottsford Miller J, Pradeep S, Taylor M, Choi H, Hansen J, et al. FAK regulates platelet extravasation and tumor growth after antiangiogenic therapy withdrawal. J Clin Invest. 2016;126:1885-96 pubmed publisher
  • western blot; mouse; 1:5000; fig 1c
Katlinskaya Y, Katlinski K, Yu Q, Ortiz A, Beiting D, Brice A, et al. Suppression of Type I Interferon Signaling Overcomes Oncogene-Induced Senescence and Mediates Melanoma Development and Progression. Cell Rep. 2016;15:171-180 pubmed publisher
  • western blot; human; 1:1000; fig s2
Wu S, Rupaimoole R, Shen F, Pradeep S, Pecot C, Ivan C, et al. A miR-192-EGR1-HOXB9 regulatory network controls the angiogenic switch in cancer. Nat Commun. 2016;7:11169 pubmed publisher
  • western blot; mouse; 1:5000; fig 5
Nemeth T, Futosi K, Sitaru C, Ruland J, Mocsai A. Neutrophil-specific deletion of the CARD9 gene expression regulator suppresses autoantibody-induced inflammation in vivo. Nat Commun. 2016;7:11004 pubmed publisher
  • western blot; human; fig 6
Mosadeghi R, Reichermeier K, Winkler M, Schreiber A, Reitsma J, Zhang Y, et al. Structural and kinetic analysis of the COP9-Signalosome activation and the cullin-RING ubiquitin ligase deneddylation cycle. elife. 2016;5: pubmed publisher
  • western blot; human; 1:5000; fig 1
Iimori M, Watanabe S, Kiyonari S, Matsuoka K, Sakasai R, Saeki H, et al. Phosphorylation of EB2 by Aurora B and CDK1 ensures mitotic progression and genome stability. Nat Commun. 2016;7:11117 pubmed publisher
  • western blot; human; 1:5000; fig 7
Woodfield S, Guo R, Liu Y, Major A, Hollingsworth E, Indiviglio S, et al. Neuroblastoma patient outcomes, tumor differentiation, and ERK activation are correlated with expression levels of the ubiquitin ligase UBE4B. Genes Cancer. 2016;7:13-26 pubmed
  • western blot; mouse; fig 1
Vermeer D, Coppock J, Zeng E, Lee K, Spanos W, Onken M, et al. Metastatic model of HPV+ oropharyngeal squamous cell carcinoma demonstrates heterogeneity in tumor metastasis. Oncotarget. 2016;7:24194-207 pubmed publisher
  • western blot; mouse; 1:5000; fig s4a
Shih Y, Hsueh Y. VCP and ATL1 regulate endoplasmic reticulum and protein synthesis for dendritic spine formation. Nat Commun. 2016;7:11020 pubmed publisher
  • western blot; human; 1:5000; fig 4
Tögel L, Nightingale R, Chueh A, Jayachandran A, Tran H, Phesse T, et al. Dual Targeting of Bromodomain and Extraterminal Domain Proteins, and WNT or MAPK Signaling, Inhibits c-MYC Expression and Proliferation of Colorectal Cancer Cells. Mol Cancer Ther. 2016;15:1217-26 pubmed publisher
  • western blot; mouse; fig 6
Van De Pette M, Tunster S, McNamara G, Shelkovnikova T, Millership S, Benson L, et al. Cdkn1c Boosts the Development of Brown Adipose Tissue in a Murine Model of Silver Russell Syndrome. PLoS Genet. 2016;12:e1005916 pubmed publisher
  • western blot; rat; 1:5000; fig 6a
Barja Fernández S, Folgueira C, Castelao C, Al Massadi O, Bravo S, Garcia Caballero T, et al. FNDC5 is produced in the stomach and associated to body composition. Sci Rep. 2016;6:23067 pubmed publisher
  • western blot; mouse; fig s1
Ma X, Xu L, Mueller E. Forkhead box A3 mediates glucocorticoid receptor function in adipose tissue. Proc Natl Acad Sci U S A. 2016;113:3377-82 pubmed publisher
  • western blot; mouse; fig 4
Shin J, Nunomiya A, Kitajima Y, Dan T, Miyata T, Nagatomi R. Prolyl hydroxylase domain 2 deficiency promotes skeletal muscle fiber-type transition via a calcineurin/NFATc1-dependent pathway. Skelet Muscle. 2016;6:5 pubmed publisher
  • western blot; human; fig 5
Pokharel D, Padula M, Lu J, Jaiswal R, Djordjevic S, Bebawy M. The Role of CD44 and ERM Proteins in Expression and Functionality of P-glycoprotein in Breast Cancer Cells. Molecules. 2016;21:290 pubmed publisher
  • western blot; mouse; 1:1000; fig s16
Karuppagounder S, Alim I, Khim S, Bourassa M, Sleiman S, John R, et al. Therapeutic targeting of oxygen-sensing prolyl hydroxylases abrogates ATF4-dependent neuronal death and improves outcomes after brain hemorrhage in several rodent models. Sci Transl Med. 2016;8:328ra29 pubmed publisher
  • western blot; rat; 1:2000; fig 2
Cannavo A, Liccardo D, Eguchi A, Elliott K, Traynham C, Ibetti J, et al. Myocardial pathology induced by aldosterone is dependent on non-canonical activities of G protein-coupled receptor kinases. Nat Commun. 2016;7:10877 pubmed publisher
  • western blot; human; 1:5000; fig 1
Woodfield S, Zhang L, Scorsone K, Liu Y, Zage P. Binimetinib inhibits MEK and is effective against neuroblastoma tumor cells with low NF1 expression. BMC Cancer. 2016;16:172 pubmed publisher
  • western blot; mouse; fig 2
  • western blot; human; fig 1
Shin M, He Y, Marrogi E, Piperdi S, Ren L, Khanna C, et al. A RUNX2-Mediated Epigenetic Regulation of the Survival of p53 Defective Cancer Cells. PLoS Genet. 2016;12:e1005884 pubmed publisher
  • western blot; mouse; fig 1
  • western blot; human; fig 1
Weiher H, Pircher H, Jansen Dürr P, Hegenbarth S, Knolle P, Grunau S, et al. A monoclonal antibody raised against bacterially expressed MPV17 sequences shows peroxisomal, endosomal and lysosomal localisation in U2OS cells. BMC Res Notes. 2016;9:128 pubmed publisher
  • western blot; human; 1:1000; fig s1
De Herdt M, Willems S, van der Steen B, Noorlag R, Verhoef E, van Leenders G, et al. Absent and abundant MET immunoreactivity is associated with poor prognosis of patients with oral and oropharyngeal squamous cell carcinoma. Oncotarget. 2016;7:13167-81 pubmed publisher
  • western blot; mouse; fig 3
  • western blot; human; fig 3
Liu L, Liu X, Ren X, Tian Y, Chen Z, Xu X, et al. Smad2 and Smad3 have differential sensitivity in relaying TGFβ signaling and inversely regulate early lineage specification. Sci Rep. 2016;6:21602 pubmed publisher
  • western blot; human; 1:30,000; fig 3
Edalat L, Stegen B, Klumpp L, Haehl E, Schilbach K, Lukowski R, et al. BK K+ channel blockade inhibits radiation-induced migration/brain infiltration of glioblastoma cells. Oncotarget. 2016;7:14259-78 pubmed publisher
  • western blot; mouse; 1:10,000; fig 2
del Río C, Navarrete C, Collado J, Bellido M, Gómez Cañas M, Pazos M, et al. The cannabinoid quinol VCE-004.8 alleviates bleomycin-induced scleroderma and exerts potent antifibrotic effects through peroxisome proliferator-activated receptor-γ and CB2 pathways. Sci Rep. 2016;6:21703 pubmed publisher
  • western blot; mouse; 1:25,000; loading ...; fig 4c
Sparks L, Gemmink A, Phielix E, Bosma M, Schaart G, Moonen Kornips E, et al. ANT1-mediated fatty acid-induced uncoupling as a target for improving myocellular insulin sensitivity. Diabetologia. 2016;59:1030-9 pubmed publisher
  • western blot; human; fig 2
Trivedi P, Bruns T, Ward S, Mai M, Schmidt C, Hirschfield G, et al. Intestinal CCL25 expression is increased in colitis and correlates with inflammatory activity. J Autoimmun. 2016;68:98-104 pubmed publisher
  • western blot; human; fig 7
Xu J, Wang N, Luo J, Xia J. Syntabulin regulates the trafficking of PICK1-containing vesicles in neurons. Sci Rep. 2016;6:20924 pubmed publisher
  • western blot; human; fig 1
Li J, Pan Q, Rowan P, Trotter T, Peker D, Regal K, et al. Heparanase promotes myeloma progression by inducing mesenchymal features and motility of myeloma cells. Oncotarget. 2016;7:11299-309 pubmed publisher
  • western blot; rat; 1:30,000; fig 3
Zhang J, Jiang Z, Bao C, Mei J, Zhu J. Cardiopulmonary bypass increases pulmonary microvascular permeability through the Src kinase pathway: Involvement of caveolin-1 and vascular endothelial cadherin. Mol Med Rep. 2016;13:2918-24 pubmed publisher
  • western blot; human; fig 1b
Heir P, Srikumar T, Bikopoulos G, Bunda S, Poon B, Lee J, et al. Oxygen-dependent Regulation of Erythropoietin Receptor Turnover and Signaling. J Biol Chem. 2016;291:7357-72 pubmed publisher
  • western blot; mouse; 1:1000; fig 1
Xu C, Ochi H, Fukuda T, Sato S, Sunamura S, Takarada T, et al. Circadian Clock Regulates Bone Resorption in Mice. J Bone Miner Res. 2016;31:1344-55 pubmed publisher
  • western blot; human; fig 1b
Jiang P, Gan M, Yen S, Moussaud S, McLean P, Dickson D. Proaggregant nuclear factor(s) trigger rapid formation of ?-synuclein aggregates in apoptotic neurons. Acta Neuropathol. 2016;132:77-91 pubmed publisher
  • western blot; human; fig 1
  • western blot; mouse; fig 3
Duplan E, Giordano C, Checler F, Alves da Costa C. Direct α-synuclein promoter transactivation by the tumor suppressor p53. Mol Neurodegener. 2016;11:13 pubmed publisher
  • western blot; human; fig 4
Hayashi H, Al Mamun A, Sakima M, Sato M. Activator of G-protein signaling 8 is involved in VEGF-mediated signal processing during angiogenesis. J Cell Sci. 2016;129:1210-22 pubmed publisher
  • western blot; mouse; 1:1000; fig 3
Blanco E, Galeano P, Palomino A, Pavón F, Rivera P, Serrano A, et al. Cocaine-induced behavioral sensitization decreases the expression of endocannabinoid signaling-related proteins in the mouse hippocampus. Eur Neuropsychopharmacol. 2016;26:477-92 pubmed publisher
  • western blot; mouse; fig 5
Park W, Kim S, Kim Y, Park J. Bortezomib alleviates drug-induced liver injury by regulating CYP2E1 gene transcription. Int J Mol Med. 2016;37:613-22 pubmed publisher
  • western blot; human; 1:5000; fig 3
Soo Lee N, Jin Chung H, Kim H, Yun Lee S, Ji J, Seo Y, et al. TRAIP/RNF206 is required for recruitment of RAP80 to sites of DNA damage. Nat Commun. 2016;7:10463 pubmed publisher
  • western blot; mouse; 1:4000; fig 2
Wu H, Shi L, Wang Q, Cheng L, Zhao X, Chen Q, et al. Mumps virus-induced innate immune responses in mouse Sertoli and Leydig cells. Sci Rep. 2016;6:19507 pubmed publisher
  • western blot; human; fig 4
Vlčková K, Ondrušová L, Vachtenheim J, Réda J, Dundr P, Zadinová M, et al. Survivin, a novel target of the Hedgehog/GLI signaling pathway in human tumor cells. Cell Death Dis. 2016;7:e2048 pubmed publisher
  • western blot; human; 1:30,000; fig 1
Klawitter S, Fuchs N, Upton K, Muñoz Lopez M, Shukla R, Wang J, et al. Reprogramming triggers endogenous L1 and Alu retrotransposition in human induced pluripotent stem cells. Nat Commun. 2016;7:10286 pubmed publisher
  • western blot; human; fig 1D
Ding X, Jiang W, Zhou P, Liu L, Wan X, Yuan X, et al. Mixed Lineage Leukemia 5 (MLL5) Protein Stability Is Cooperatively Regulated by O-GlcNac Transferase (OGT) and Ubiquitin Specific Protease 7 (USP7). PLoS ONE. 2015;10:e0145023 pubmed publisher
  • western blot; human; fig 2
Monian P, Jiang X. The Cellular Apoptosis Susceptibility Protein (CAS) Promotes Tumor Necrosis Factor-related Apoptosis-inducing Ligand (TRAIL)-induced Apoptosis and Cell Proliferation. J Biol Chem. 2016;291:2379-88 pubmed publisher
  • western blot; human; 1:4000; fig 2b
Weijer R, Broekgaarden M, Krekorian M, Alles L, van Wijk A, Mackaaij C, et al. Inhibition of hypoxia inducible factor 1 and topoisomerase with acriflavine sensitizes perihilar cholangiocarcinomas to photodynamic therapy. Oncotarget. 2016;7:3341-56 pubmed publisher
  • western blot; human; fig 1
Adam M, Matt S, Christian S, Hess Stumpp H, Haegebarth A, Hofmann T, et al. SIAH ubiquitin ligases regulate breast cancer cell migration and invasion independent of the oxygen status. Cell Cycle. 2015;14:3734-47 pubmed publisher
  • western blot; human; fig 2
Iskit S, Schlicker A, Wessels L, Peeper D. Fra-1 is a key driver of colon cancer metastasis and a Fra-1 classifier predicts disease-free survival. Oncotarget. 2015;6:43146-61 pubmed publisher
  • western blot; mouse; 1:10,000; fig 1
Hashimoto M, Murata K, Ishida J, Kanou A, Kasuya Y, Fukamizu A. Severe Hypomyelination and Developmental Defects Are Caused in Mice Lacking Protein Arginine Methyltransferase 1 (PRMT1) in the Central Nervous System. J Biol Chem. 2016;291:2237-45 pubmed publisher
  • western blot; mouse; 1:1000; fig 5
Min J, Guo K, Suryadevara P, Zhu F, Holbrook G, Chen Y, et al. Optimization of a Novel Series of Ataxia-Telangiectasia Mutated Kinase Inhibitors as Potential Radiosensitizing Agents. J Med Chem. 2016;59:559-77 pubmed publisher
  • western blot; human; 1:10,000; fig 2
  • western blot; mouse; 1:10,000; fig 2
Bunda S, Burrell K, Heir P, Zeng L, Alamsahebpour A, Kano Y, et al. Inhibition of SHP2-mediated dephosphorylation of Ras suppresses oncogenesis. Nat Commun. 2015;6:8859 pubmed publisher
  • western blot; mouse; fig 4d
Hagelkruys A, Mattes K, Moos V, Rennmayr M, Ringbauer M, Sawicka A, et al. Essential Nonredundant Function of the Catalytic Activity of Histone Deacetylase 2 in Mouse Development. Mol Cell Biol. 2016;36:462-74 pubmed publisher
  • western blot; human; fig 1
Kim M, Kim M, Park S, Lee C, Lim D. Role of Angiomotin-like 2 mono-ubiquitination on YAP inhibition. EMBO Rep. 2016;17:64-78 pubmed publisher
  • western blot; rat; fig 1
Wang C, Wang Y, Hu M, Chai Z, Wu Q, Huang R, et al. Synaptotagmin-11 inhibits clathrin-mediated and bulk endocytosis. EMBO Rep. 2016;17:47-63 pubmed publisher
  • western blot; mouse; fig 1
Gao L, Jiang Y, Mu L, Liu Y, Wang F, Wang P, et al. Efficient Generation of Mice with Consistent Transgene Expression by FEEST. Sci Rep. 2015;5:16284 pubmed publisher
  • western blot; human; fig 2
Amigo Jiménez I, Bailón E, Aguilera Montilla N, Terol M, García Marco J, García Pardo A. Bone marrow stroma-induced resistance of chronic lymphocytic leukemia cells to arsenic trioxide involves Mcl-1 upregulation and is overcome by inhibiting the PI3Kδ or PKCβ signaling pathways. Oncotarget. 2015;6:44832-48 pubmed publisher
  • western blot; human; fig 3,4,5
Elgner F, Donnerhak C, Ren H, Medvedev R, Schreiber A, Weber L, et al. Characterization of α-taxilin as a novel factor controlling the release of hepatitis C virus. Biochem J. 2016;473:145-55 pubmed publisher
  • western blot; human; fig 1
Shi J, Fung G, Deng H, Zhang J, Fiesel F, Springer W, et al. NBR1 is dispensable for PARK2-mediated mitophagy regardless of the presence or absence of SQSTM1. Cell Death Dis. 2015;6:e1943 pubmed publisher
  • western blot; human; fig 1
  • western blot; dogs; fig 1
Heishima K, Mori T, Ichikawa Y, Sakai H, Kuranaga Y, Nakagawa T, et al. MicroRNA-214 and MicroRNA-126 Are Potential Biomarkers for Malignant Endothelial Proliferative Diseases. Int J Mol Sci. 2015;16:25377-91 pubmed publisher
  • western blot; mouse; 1:5000; fig s1
Gökbuget D, Pereira J, Bachofner S, Marchais A, Ciaudo C, Stoffel M, et al. The Lin28/let-7 axis is critical for myelination in the peripheral nervous system. Nat Commun. 2015;6:8584 pubmed publisher
  • western blot; human; fig 1b
Kabaria S, Choi D, Chaudhuri A, Jain M, Li H, Junn E. MicroRNA-7 activates Nrf2 pathway by targeting Keap1 expression. Free Radic Biol Med. 2015;89:548-56 pubmed publisher
  • western blot; mouse; fig 7
  • western blot; human; fig 1
Yu D, Makkar G, Strickland D, Blanpied T, Stumpo D, Blackshear P, et al. Myristoylated Alanine-Rich Protein Kinase Substrate (MARCKS) Regulates Small GTPase Rac1 and Cdc42 Activity and Is a Critical Mediator of Vascular Smooth Muscle Cell Migration in Intimal Hyperplasia Formation. J Am Heart Assoc. 2015;4:e002255 pubmed publisher
  • western blot; human; 1:3000; fig 2b
  • western blot; mouse; 1:3000; fig 2b
André P, Prêle C, Vierkotten S, Carnesecchi S, Donati Y, Chambers R, et al. BARD1 mediates TGF-β signaling in pulmonary fibrosis. Respir Res. 2015;16:118 pubmed publisher
  • western blot; rat; fig 1
Pellet Many C, Mehta V, Fields L, Mahmoud M, Lowe V, Evans I, et al. Neuropilins 1 and 2 mediate neointimal hyperplasia and re-endothelialization following arterial injury. Cardiovasc Res. 2015;108:288-98 pubmed publisher
  • western blot; human; 1:5000; fig 3d
Mazur P, Herner A, Mello S, Wirth M, Hausmann S, Sánchez Rivera F, et al. Combined inhibition of BET family proteins and histone deacetylases as a potential epigenetics-based therapy for pancreatic ductal adenocarcinoma. Nat Med. 2015;21:1163-71 pubmed publisher
  • western blot; mouse; 1:50,000; fig 1
Nemazanyy I, Montagnac G, Russell R, Morzyglod L, Burnol A, Guan K, et al. Class III PI3K regulates organismal glucose homeostasis by providing negative feedback on hepatic insulin signalling. Nat Commun. 2015;6:8283 pubmed publisher
  • western blot; mouse; fig 3
Yang L, McKnight G. Hypothalamic PKA regulates leptin sensitivity and adiposity. Nat Commun. 2015;6:8237 pubmed publisher
  • western blot; human; fig 3
Zoppi N, Chiarelli N, Cinquina V, Ritelli M, Colombi M. GLUT10 deficiency leads to oxidative stress and non-canonical αvβ3 integrin-mediated TGFβ signalling associated with extracellular matrix disarray in arterial tortuosity syndrome skin fibroblasts. Hum Mol Genet. 2015;24:6769-87 pubmed publisher
  • western blot; bovine; 1:1000; fig 1b
Laird J, Pan Y, Modestou M, Yamaguchi D, Song H, Sokolov M, et al. Identification of a VxP Targeting Signal in the Flagellar Na+ /K+ -ATPase. Traffic. 2015;16:1239-53 pubmed publisher
  • western blot; human
Qiao Y, Lin S, Chen Y, Voon D, Zhu F, Chuang L, et al. RUNX3 is a novel negative regulator of oncogenic TEAD-YAP complex in gastric cancer. Oncogene. 2016;35:2664-74 pubmed publisher
  • western blot; rat
Li M, Yang S, Xing B, Ferguson B, Gulchina Y, Li Y, et al. LY395756, an mGluR2 agonist and mGluR3 antagonist, enhances NMDA receptor expression and function in the normal adult rat prefrontal cortex, but fails to improve working memory and reverse MK801-induced working memory impairment. Exp Neurol. 2015;273:190-201 pubmed publisher
  • western blot; human; fig 8
Heishima K, Mori T, Sakai H, Sugito N, Murakami M, Yamada N, et al. MicroRNA-214 Promotes Apoptosis in Canine Hemangiosarcoma by Targeting the COP1-p53 Axis. PLoS ONE. 2015;10:e0137361 pubmed publisher
  • western blot; mouse
Pflibsen L, Stang K, Sconce M, Wilson V, Hood R, Meshul C, et al. Executive function deficits and glutamatergic protein alterations in a progressive 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine mouse model of Parkinson's disease. J Neurosci Res. 2015;93:1849-64 pubmed publisher
  • western blot; human; fig 5a
Wu X, Zahari M, Renuse S, Nirujogi R, Kim M, Manda S, et al. Phosphoproteomic Analysis Identifies Focal Adhesion Kinase 2 (FAK2) as a Potential Therapeutic Target for Tamoxifen Resistance in Breast Cancer. Mol Cell Proteomics. 2015;14:2887-900 pubmed publisher
  • western blot; human; loading ...; fig st2
Tiffen J, Gunatilake D, Gallagher S, Gowrishankar K, Heinemann A, Cullinane C, et al. Targeting activating mutations of EZH2 leads to potent cell growth inhibition in human melanoma by derepression of tumor suppressor genes. Oncotarget. 2015;6:27023-36 pubmed publisher
  • western blot; human
Kauko O, Laajala T, Jumppanen M, Hintsanen P, Suni V, Haapaniemi P, et al. Label-free quantitative phosphoproteomics with novel pairwise abundance normalization reveals synergistic RAS and CIP2A signaling. Sci Rep. 2015;5:13099 pubmed publisher
  • western blot; human; fig 1
Kieffer Kwon P, Happel C, Uldrick T, Ramalingam D, Ziegelbauer J. KSHV MicroRNAs Repress Tropomyosin 1 and Increase Anchorage-Independent Growth and Endothelial Tube Formation. PLoS ONE. 2015;10:e0135560 pubmed publisher
  • western blot; human; 1:10000; fig s13
Swindell W, Remmer H, Sarkar M, Xing X, Barnes D, Wolterink L, et al. Proteogenomic analysis of psoriasis reveals discordant and concordant changes in mRNA and protein abundance. Genome Med. 2015;7:86 pubmed publisher
  • western blot; human; 1:2000; fig 1
Roggia M, Ueta T. αvβ5 Integrin/FAK/PGC-1α Pathway Confers Protective Effects on Retinal Pigment Epithelium. PLoS ONE. 2015;10:e0134870 pubmed publisher
  • western blot; mouse; fig 4b
Hieke N, Löffler A, Kaizuka T, Berleth N, Böhler P, Drießen S, et al. Expression of a ULK1/2 binding-deficient ATG13 variant can partially restore autophagic activity in ATG13-deficient cells. Autophagy. 2015;11:1471-83 pubmed publisher
  • western blot; human
Kiyonari S, Iimori M, Matsuoka K, Watanabe S, Morikawa Ichinose T, Miura D, et al. The 1,2-Diaminocyclohexane Carrier Ligand in Oxaliplatin Induces p53-Dependent Transcriptional Repression of Factors Involved in Thymidylate Biosynthesis. Mol Cancer Ther. 2015;14:2332-42 pubmed publisher
  • western blot; human; fig 1a,b,d
Drießen S, Berleth N, Friesen O, Löffler A, Böhler P, Hieke N, et al. Deubiquitinase inhibition by WP1130 leads to ULK1 aggregation and blockade of autophagy. Autophagy. 2015;11:1458-70 pubmed publisher
  • western blot; human; 1:5000; fig 1c
  • western blot; mouse; 1:5000; fig 1b
Lee M, Jeong M, Lee H, Han H, Ko A, Hewitt S, et al. PI3K/AKT activation induces PTEN ubiquitination and destabilization accelerating tumourigenesis. Nat Commun. 2015;6:7769 pubmed publisher
  • western blot; mouse; 1:5000; fig 1d
Quintana P, Soto D, Poirot O, Zonouzi M, Kellenberger S, Muller D, et al. Acid-sensing ion channel 1a drives AMPA receptor plasticity following ischaemia and acidosis in hippocampal CA1 neurons. J Physiol. 2015;593:4373-86 pubmed publisher
  • western blot; human; 1:5000; fig 1
Kontro H, Cannino G, Rustin P, Dufour E, Kainulainen H. DAPIT Over-Expression Modulates Glucose Metabolism and Cell Behaviour in HEK293T Cells. PLoS ONE. 2015;10:e0131990 pubmed publisher
  • western blot; mouse; 1:4000; fig 1
Wu C, Jiao H, Lai Y, Zheng W, Chen K, Qu H, et al. Kindlin-2 controls TGF-β signalling and Sox9 expression to regulate chondrogenesis. Nat Commun. 2015;6:7531 pubmed publisher
  • western blot; mouse; 1:2000; fig 2
Kim A, Park Y, Pan X, Shin K, Kwak S, Bassas A, et al. Obesity-induced DNA hypermethylation of the adiponectin gene mediates insulin resistance. Nat Commun. 2015;6:7585 pubmed publisher
  • western blot; rat; fig 1
Jacquin S, Rincheval V, Mignotte B, Richard S, Humbert M, Mercier O, et al. Inactivation of p53 Is Sufficient to Induce Development of Pulmonary Hypertension in Rats. PLoS ONE. 2015;10:e0131940 pubmed publisher
  • western blot; human; fig 2
Chhabra S, Jain S, Wallace C, Hong F, Liu B. High expression of endoplasmic reticulum chaperone grp94 is a novel molecular hallmark of malignant plasma cells in multiple myeloma. J Hematol Oncol. 2015;8:77 pubmed publisher
  • western blot; mouse; 1:5000
Gopinathan G, Milagre C, Pearce O, Reynolds L, Hodivala Dilke K, Leinster D, et al. Interleukin-6 Stimulates Defective Angiogenesis. Cancer Res. 2015;75:3098-107 pubmed publisher
  • western blot; mouse; fig 2
Kim J, Jeong M, Lee S, Song J. Camptothecin and topotecan inhibit adipocyte differentiation by inducing degradation of PPARγ. Biochem Biophys Res Commun. 2015;463:1122-8 pubmed publisher
  • western blot; rat; 1:4000
Ronchi G, Haastert Talini K, Fornasari B, Perroteau I, Geuna S, Gambarotta G. The Neuregulin1/ErbB system is selectively regulated during peripheral nerve degeneration and regeneration. Eur J Neurosci. 2016;43:351-64 pubmed publisher
  • western blot; human
de Wilde A, Wannee K, Scholte F, Goeman J, Ten Dijke P, Snijder E, et al. A Kinome-Wide Small Interfering RNA Screen Identifies Proviral and Antiviral Host Factors in Severe Acute Respiratory Syndrome Coronavirus Replication, Including Double-Stranded RNA-Activated Protein Kinase and Early Secretory Pathway Proteins. J Virol. 2015;89:8318-33 pubmed publisher
  • western blot; human
Barber A, Castillo Martin M, Bonal D, Jia A, Rybicki B, Christiano A, et al. PI3K/AKT pathway regulates E-cadherin and Desmoglein 2 in aggressive prostate cancer. Cancer Med. 2015;4:1258-71 pubmed publisher
  • western blot; human; fig 5
Bosco D, Kenworthy R, Zorio D, Sang Q. Human mesenchymal stem cells are resistant to Paclitaxel by adopting a non-proliferative fibroblastic state. PLoS ONE. 2015;10:e0128511 pubmed publisher
  • western blot; human; fig 1
Park S, Shim J, Park H, Eum D, Park M, Mi Yi J, et al. MacroH2A1 downregulation enhances the stem-like properties of bladder cancer cells by transactivation of Lin28B. Oncogene. 2016;35:1292-301 pubmed publisher
  • western blot; mouse
Musicki B, Zhang Y, Chen H, Brown T, Zirkin B, Burnett A. Mechanism of testosterone deficiency in the transgenic sickle cell mouse. PLoS ONE. 2015;10:e0128694 pubmed publisher
  • western blot; human; 1:5000; fig 1b
Yu J, Ramasamy T, Murphy N, Holt M, Czapiewski R, Wei S, et al. PI3K/mTORC2 regulates TGF-β/Activin signalling by modulating Smad2/3 activity via linker phosphorylation. Nat Commun. 2015;6:7212 pubmed publisher
  • western blot; mouse; 1:500; fig 2e
Watanabe S, Sanuki R, Sugita Y, Imai W, Yamazaki R, Kozuka T, et al. Prdm13 regulates subtype specification of retinal amacrine interneurons and modulates visual sensitivity. J Neurosci. 2015;35:8004-20 pubmed publisher
  • western blot; human
Yang C, Chiang C, Chen C, Lee Y, Wu M, Tsou Y, et al. Identification and characterization of nuclear and nucleolar localization signals in 58-kDa microspherule protein (MSP58). J Biomed Sci. 2015;22:33 pubmed publisher
  • western blot; human; fig 6
Wong T, Lin S, Leung L. The flavone apigenin blocks nuclear translocation of sterol regulatory element-binding protein-2 in the hepatic cells WRL-68. Br J Nutr. 2015;113:1844-52 pubmed publisher
  • western blot; human
Garcia P, Miller A, Kreitzburg K, Council L, Gamblin T, Christein J, et al. The BET bromodomain inhibitor JQ1 suppresses growth of pancreatic ductal adenocarcinoma in patient-derived xenograft models. Oncogene. 2016;35:833-45 pubmed publisher
  • western blot; mouse; fig 6
Lin A, Beasley F, Olson J, Keller N, Shalwitz R, Hannan T, et al. Role of Hypoxia Inducible Factor-1α (HIF-1α) in Innate Defense against Uropathogenic Escherichia coli Infection. PLoS Pathog. 2015;11:e1004818 pubmed publisher
  • western blot; human; fig 3
Studzian M, Bartosz G, Pulaski L. Endocytosis of ABCG2 drug transporter caused by binding of 5D3 antibody: trafficking mechanisms and intracellular fate. Biochim Biophys Acta. 2015;1853:1759-71 pubmed publisher
  • western blot; human; 1:5000; fig 1
Suzuki M, Takeda T, Nakagawa H, Iwata S, Watanabe T, Siddiquey M, et al. The heat shock protein 90 inhibitor BIIB021 suppresses the growth of T and natural killer cell lymphomas. Front Microbiol. 2015;6:280 pubmed publisher
  • western blot; mouse; 1:2000; fig 6
Sheean R, Weston R, Perera N, D Amico A, Nutt S, Turner B. Effect of thymic stimulation of CD4+ T cell expansion on disease onset and progression in mutant SOD1 mice. J Neuroinflammation. 2015;12:40 pubmed publisher
  • western blot; mouse; fig 2
Uetake Y, Ikeda H, Irie R, Tejima K, Matsui H, Ogura S, et al. High-salt in addition to high-fat diet may enhance inflammation and fibrosis in liver steatosis induced by oxidative stress and dyslipidemia in mice. Lipids Health Dis. 2015;14:6 pubmed publisher
  • western blot; human; fig 1
Vishwamitra D, Curry C, Alkan S, Song Y, Gallick G, Kaseb A, et al. The transcription factors Ik-1 and MZF1 downregulate IGF-IR expression in NPM-ALK⁺ T-cell lymphoma. Mol Cancer. 2015;14:53 pubmed publisher
  • western blot; human; 1:5000
Basu S, Thorat R, Dalal S. MMP7 is required to mediate cell invasion and tumor formation upon Plakophilin3 loss. PLoS ONE. 2015;10:e0123979 pubmed publisher
  • western blot; human; 1:20,000; fig 1
Jühlen R, Idkowiak J, Taylor A, Kind B, Arlt W, Huebner A, et al. Role of ALADIN in human adrenocortical cells for oxidative stress response and steroidogenesis. PLoS ONE. 2015;10:e0124582 pubmed publisher
  • western blot; mouse; fig 3
Kropp K, Hsieh W, Isern E, Forster T, Krause E, Brune W, et al. A temporal gate for viral enhancers to co-opt Toll-like-receptor transcriptional activation pathways upon acute infection. PLoS Pathog. 2015;11:e1004737 pubmed publisher
  • western blot; mouse; fig 4
Vashist S, Ureña L, Gonzalez Hernandez M, Choi J, de Rougemont A, Rocha Pereira J, et al. Molecular chaperone Hsp90 is a therapeutic target for noroviruses. J Virol. 2015;89:6352-63 pubmed publisher
  • western blot; human; fig 2
Schilling D, Kühnel A, Tetzlaff F, Konrad S, Multhoff G. NZ28-induced inhibition of HSF1, SP1 and NF-κB triggers the loss of the natural killer cell-activating ligands MICA/B on human tumor cells. Cancer Immunol Immunother. 2015;64:599-608 pubmed publisher
  • western blot; human; fig 1
Hausmann S, Brandt E, Köchel C, Einsele H, Bargou R, Seggewiss Bernhardt R, et al. Loss of serum and glucocorticoid-regulated kinase 3 (SGK3) does not affect proliferation and survival of multiple myeloma cell lines. PLoS ONE. 2015;10:e0122689 pubmed publisher
  • western blot; rat; fig 2
Neira Peña T, Rojas Mancilla E, Munoz Vio V, Perez R, Gutierrez Hernandez M, Bustamante D, et al. Perinatal asphyxia leads to PARP-1 overactivity, p65 translocation, IL-1β and TNF-α overexpression, and apoptotic-like cell death in mesencephalon of neonatal rats: prevention by systemic neonatal nicotinamide administration. Neurotox Res. 2015;27:453-65 pubmed publisher
  • western blot; mouse; fig 2
Yamakoshi K, Katano S, Iida M, Kimura H, Okuma A, Ikemoto Uezumi M, et al. Dysregulation of the Bmi-1/p16(Ink⁴a) pathway provokes an aging-associated decline of submandibular gland function. Aging Cell. 2015;14:616-24 pubmed publisher
  • western blot; human; fig 4
Yun H, Xie J, Olumi A, Ghosh R, Kumar A. Activation of AKR1C1/ERβ induces apoptosis by downregulation of c-FLIP in prostate cancer cells: A prospective therapeutic opportunity. Oncotarget. 2015;6:11600-13 pubmed
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Hsieh C, Botta G, Gao S, Li T, Van Allen E, Treacy D, et al. PLZF, a tumor suppressor genetically lost in metastatic castration-resistant prostate cancer, is a mediator of resistance to androgen deprivation therapy. Cancer Res. 2015;75:1944-8 pubmed publisher
  • western blot; human; fig s5
Chu V, Weber T, Wefers B, Wurst W, Sander S, Rajewsky K, et al. Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. Nat Biotechnol. 2015;33:543-8 pubmed publisher
  • western blot; mouse; 1:2000; fig s2
Venkatesh A, Ma S, Le Y, Hall M, Rüegg M, Punzo C. Activated mTORC1 promotes long-term cone survival in retinitis pigmentosa mice. J Clin Invest. 2015;125:1446-58 pubmed publisher
  • western blot; human; fig 2
Shibayama Y, Kondo T, Ohya H, Fujisawa S, Teshima T, Iseki K. Upregulation of microRNA-126-5p is associated with drug resistance to cytarabine and poor prognosis in AML patients. Oncol Rep. 2015;33:2176-82 pubmed publisher
  • western blot; human; 1:30000
López González I, Schlüter A, Aso E, Garcia Esparcia P, Ansoleaga B, Llorens F, et al. Neuroinflammatory signals in Alzheimer disease and APP/PS1 transgenic mice: correlations with plaques, tangles, and oligomeric species. J Neuropathol Exp Neurol. 2015;74:319-44 pubmed publisher
  • western blot; human; fig 1
Warren D, Tajsic T, Porter L, Minaisah R, Cobb A, Jacob A, et al. Nesprin-2-dependent ERK1/2 compartmentalisation regulates the DNA damage response in vascular smooth muscle cell ageing. Cell Death Differ. 2015;22:1540-50 pubmed publisher
  • western blot; mouse; fig 2
Aubrey B, Kelly G, Kueh A, Brennan M, O Connor L, Milla L, et al. An inducible lentiviral guide RNA platform enables the identification of tumor-essential genes and tumor-promoting mutations in vivo. Cell Rep. 2015;10:1422-32 pubmed publisher
  • western blot; human
Prabhu A, Sarcar B, Miller C, Kim S, Nakano I, Forsyth P, et al. Ras-mediated modulation of pyruvate dehydrogenase activity regulates mitochondrial reserve capacity and contributes to glioblastoma tumorigenesis. Neuro Oncol. 2015;17:1220-30 pubmed publisher
  • western blot; mouse; 1:5000; fig s4
Mourier A, Motori E, Brandt T, Lagouge M, Atanassov I, Galinier A, et al. Mitofusin 2 is required to maintain mitochondrial coenzyme Q levels. J Cell Biol. 2015;208:429-42 pubmed publisher
  • western blot; mouse; fig 4
Guillaumond F, Bidaut G, Ouaissi M, Servais S, Gouirand V, Olivares O, et al. Cholesterol uptake disruption, in association with chemotherapy, is a promising combined metabolic therapy for pancreatic adenocarcinoma. Proc Natl Acad Sci U S A. 2015;112:2473-8 pubmed publisher
  • western blot; mouse; fig 1
Michelet X, Garg S, Wolf B, Tuli A, Ricciardi Castagnoli P, Brenner M. MHC class II presentation is controlled by the lysosomal small GTPase, Arl8b. J Immunol. 2015;194:2079-88 pubmed publisher
  • western blot; mouse; 1:20000; fig 1
La Sala G, Marazziti D, Di Pietro C, Golini E, Matteoni R, Tocchini Valentini G. Modulation of Dhh signaling and altered Sertoli cell function in mice lacking the GPR37-prosaposin receptor. FASEB J. 2015;29:2059-69 pubmed publisher
  • western blot; mouse; 1:10,000; fig 1
Lu W, Liu S, Li B, Xie Y, Adhiambo C, Yang Q, et al. SKP2 inactivation suppresses prostate tumorigenesis by mediating JARID1B ubiquitination. Oncotarget. 2015;6:771-88 pubmed
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Wang W, Visavadiya N, Pandya J, Nelson P, Sullivan P, Springer J. Mitochondria-associated microRNAs in rat hippocampus following traumatic brain injury. Exp Neurol. 2015;265:84-93 pubmed publisher
  • western blot; human
Jepsen M, Kløverpris S, Mikkelsen J, Pedersen J, Füchtbauer E, Laursen L, et al. Stanniocalcin-2 inhibits mammalian growth by proteolytic inhibition of the insulin-like growth factor axis. J Biol Chem. 2015;290:3430-9 pubmed publisher
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Han Y, Hsieh F. Osteogenic differentiation of late-outgrowth CD45-negative endothelial progenitor cells. J Vasc Res. 2014;51:369-75 pubmed publisher
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  • western blot; mouse; fig 5
Zhang D, Wang X, Li Y, Zhao L, Lu M, Yao X, et al. Thyroid hormone regulates muscle fiber type conversion via miR-133a1. J Cell Biol. 2014;207:753-66 pubmed publisher
  • western blot; human; 1:7000
Winkler M, Dib C, Ljubimov A, Saghizadeh M. Targeting miR-146a to treat delayed wound healing in human diabetic organ-cultured corneas. PLoS ONE. 2014;9:e114692 pubmed publisher
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  • western blot; mouse; 1:5000; fig 2
Ventelä S, Sittig E, Mannermaa L, Mäkelä J, Kulmala J, Löyttyniemi E, et al. CIP2A is an Oct4 target gene involved in head and neck squamous cell cancer oncogenicity and radioresistance. Oncotarget. 2015;6:144-58 pubmed
  • immunocytochemistry; mouse
Lu S, Zeumer L, Sorensen H, Yang H, Ng Y, Yu F, et al. The murine Pbx1-d lupus susceptibility allele accelerates mesenchymal stem cell differentiation and impairs their immunosuppressive function. J Immunol. 2015;194:43-55 pubmed
  • western blot; mouse; 1:5000
Nashine S, Liu Y, Kim B, Clark A, Pang I. Role of C/EBP homologous protein in retinal ganglion cell death after ischemia/reperfusion injury. Invest Ophthalmol Vis Sci. 2014;56:221-31 pubmed publisher
  • western blot; mouse; 1:10,000; fig 1
Kim H, Xu H, Yao Q, Li W, Huang Q, Outeda P, et al. Ciliary membrane proteins traffic through the Golgi via a Rabep1/GGA1/Arl3-dependent mechanism. Nat Commun. 2014;5:5482 pubmed publisher
  • western blot; mouse; 1:10,000; fig 2
Gascon E, Lynch K, Ruan H, Almeida S, Verheyden J, Seeley W, et al. Alterations in microRNA-124 and AMPA receptors contribute to social behavioral deficits in frontotemporal dementia. Nat Med. 2014;20:1444-51 pubmed publisher
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Marques Howarth M, Simpson D, Ngok S, Nieves B, Chen R, Siprashvili Z, et al. Long noncoding RNA EWSAT1-mediated gene repression facilitates Ewing sarcoma oncogenesis. J Clin Invest. 2014;124:5275-90 pubmed publisher
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Plosa E, Young L, Gulleman P, Polosukhin V, Zaynagetdinov R, Benjamin J, et al. Epithelial β1 integrin is required for lung branching morphogenesis and alveolarization. Development. 2014;141:4751-62 pubmed publisher
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Wang D, Zhang P, Gao K, Tang Y, Jin X, Zhang Y, et al. PLK1 and β-TrCP-dependent ubiquitination and degradation of Rap1GAP controls cell proliferation. PLoS ONE. 2014;9:e110296 pubmed publisher
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Bailey Elkin B, Knaap R, Johnson G, Dalebout T, Ninaber D, van Kasteren P, et al. Crystal structure of the Middle East respiratory syndrome coronavirus (MERS-CoV) papain-like protease bound to ubiquitin facilitates targeted disruption of deubiquitinating activity to demonstrate its role in innate immune suppression. J Biol Chem. 2014;289:34667-82 pubmed publisher
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Zhang Y, Wang N, Su P, Lu J, Wang Y. Disruption of dopamine D1 receptor phosphorylation at serine 421 attenuates cocaine-induced behaviors in mice. Neurosci Bull. 2014;30:1025-35 pubmed publisher
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Kinjo E, Higa G, Morya E, Valle A, Kihara A, Britto L. Reciprocal regulation of epileptiform neuronal oscillations and electrical synapses in the rat hippocampus. PLoS ONE. 2014;9:e109149 pubmed publisher
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Lee S, Xu G, Jay T, Bhatta S, Kim K, Jung S, et al. Opposing effects of membrane-anchored CX3CL1 on amyloid and tau pathologies via the p38 MAPK pathway. J Neurosci. 2014;34:12538-46 pubmed publisher
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Abazyan S, Yang E, Abazyan B, Xia M, Yang C, Rojas C, et al. Mutant disrupted-in-schizophrenia 1 in astrocytes: focus on glutamate metabolism. J Neurosci Res. 2014;92:1659-68 pubmed publisher
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Steiner E, Enzmann G, Lyck R, Lin S, Ruegg M, Kroger S, et al. The heparan sulfate proteoglycan agrin contributes to barrier properties of mouse brain endothelial cells by stabilizing adherens junctions. Cell Tissue Res. 2014;358:465-79 pubmed publisher
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Sutiwisesak R, Kitiyanant N, Kotchabhakdi N, Felsenfeld G, Andrews P, Wongtrakoongate P. Induced pluripotency enables differentiation of human nullipotent embryonal carcinoma cells N2102Ep. Biochim Biophys Acta. 2014;1843:2611-9 pubmed publisher
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Saha S, Parachoniak C, Ghanta K, Fitamant J, Ross K, Najem M, et al. Mutant IDH inhibits HNF-4α to block hepatocyte differentiation and promote biliary cancer. Nature. 2014;513:110-4 pubmed publisher
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Ouyang H, Xue Y, Lin Y, Zhang X, Xi L, Patel S, et al. WNT7A and PAX6 define corneal epithelium homeostasis and pathogenesis. Nature. 2014;511:358-61 pubmed publisher
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Kehoe L, Bellone C, De Roo M, Zandueta A, Dey P, Pérez Otaño I, et al. GluN3A promotes dendritic spine pruning and destabilization during postnatal development. J Neurosci. 2014;34:9213-21 pubmed publisher
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Lakshminarayan R, Wunder C, Becken U, Howes M, Benzing C, Arumugam S, et al. Galectin-3 drives glycosphingolipid-dependent biogenesis of clathrin-independent carriers. Nat Cell Biol. 2014;16:595-606 pubmed publisher
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Durak O, de Anda F, Singh K, Leussis M, Petryshen T, Sklar P, et al. Ankyrin-G regulates neurogenesis and Wnt signaling by altering the subcellular localization of ?-catenin. Mol Psychiatry. 2015;20:388-97 pubmed publisher
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Thoresen S, Campsteijn C, Vietri M, Schink K, Liestøl K, Andersen J, et al. ANCHR mediates Aurora-B-dependent abscission checkpoint control through retention of VPS4. Nat Cell Biol. 2014;16:550-60 pubmed publisher
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Dichamp I, Seite P, Agius G, Barbarin A, Beby Defaux A. Human papillomavirus 16 oncoprotein E7 stimulates UBF1-mediated rDNA gene transcription, inhibiting a p53-independent activity of p14ARF. PLoS ONE. 2014;9:e96136 pubmed publisher
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Tossetta G, Paolinelli F, Avellini C, Salvolini E, Ciarmela P, Lorenzi T, et al. IL-1? and TGF-? weaken the placental barrier through destruction of tight junctions: an in vivo and in vitro study. Placenta. 2014;35:509-16 pubmed publisher
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Gürtler C, Carty M, Kearney J, Schattgen S, Ding A, Fitzgerald K, et al. SARM regulates CCL5 production in macrophages by promoting the recruitment of transcription factors and RNA polymerase II to the Ccl5 promoter. J Immunol. 2014;192:4821-32 pubmed publisher
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Low Calle A, Prada Arismendy J, Castellanos J. Study of interferon-? antiviral activity against Herpes simplex virus type 1 in neuron-enriched trigeminal ganglia cultures. Virus Res. 2014;180:49-58 pubmed publisher
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Gregoraszczuk E, Ptak A, Karpeta A, Fiedor E, Wrobel A, Milewicz T, et al. Hexachlorobenzene and pentachlorobenzene accumulation, metabolism and effect on steroid secretion and on CYP11A1 and CYP19 expression in cultured human placental tissue. Reprod Toxicol. 2014;43:102-10 pubmed publisher
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Wongtrakoongate P, Li J, Andrews P. DNMT3B inhibits the re-expression of genes associated with induced pluripotency. Exp Cell Res. 2014;321:231-9 pubmed publisher
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product information
Product No. :
A5316
Product Name :
Monoclonal Anti-β-Actin antibody produced in mouse
Host :
mouse
Conjugate :
unconjugated
Antibody Type :
monoclonal
Clone Number (if monoclonal) :
AC-74
Applications/Description :
Suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections); western blot: 1:5,000 using cultured human or chicken fibroblast cell extracts; indirect immunofluorescence: 1:1,000 using cultured human or chicken fibroblasts; indirect ELISA: sui
Species Reactivity :
canine, guinea pig, rabbit, bovine, chicken, rat, sheep, carp, wide range, Drosophila, Hirudo medicinalis, pig, cat, mouse, human
Isotype :
IgG2a
Storage :
Store at -20°C
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA