product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
antibody
product name :
Monoclonal Anti-ATG5 antibody produced in mouse
catalog :
A2859
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
ATG5-18
reactivity :
human, mouse, rat
application :
western blot, immunoprecipitation, western blot knockout validation
more info or order :
citations: 6
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; 1:1000; fig 8h
  • western blot knockout validation; human; 1:1000; loading ...; fig e2a
Chen G, Xie W, Nah J, Sauvat A, Liu P, Pietrocola F, et al. 3,4-Dimethoxychalcone induces autophagy through activation of the transcription factors TFE3 and TFEB. EMBO Mol Med. 2019;11:e10469 pubmed publisher
  • western blot; mouse; fig e3
Kimmey J, Huynh J, Weiss L, Park S, Kambal A, Debnath J, et al. Unique role for ATG5 in neutrophil-mediated immunopathology during M. tuberculosis infection. Nature. 2015;528:565-9 pubmed publisher
  • western blot; mouse; fig 2
  • western blot; rat; fig 7
Li S, Wang W, Niu T, Wang H, Li B, Shao L, et al. Nrf2 deficiency exaggerates doxorubicin-induced cardiotoxicity and cardiac dysfunction. Oxid Med Cell Longev. 2014;2014:748524 pubmed publisher
Sauvat A, Chen G, Müller K, Tong M, Aprahamian F, Durand S, et al. Trans-Fats Inhibit Autophagy Induced by Saturated Fatty Acids. EBioMedicine. 2018;30:261-272 pubmed publisher
Jiang X, Li L, Ying Z, Pan C, Huang S, Li L, et al. A Small Molecule That Protects the Integrity of the Electron Transfer Chain Blocks the Mitochondrial Apoptotic Pathway. Mol Cell. 2016;63:229-239 pubmed publisher
Tumbarello D, Waxse B, Arden S, Bright N, Kendrick Jones J, Buss F. Autophagy receptors link myosin VI to autophagosomes to mediate Tom1-dependent autophagosome maturation and fusion with the lysosome. Nat Cell Biol. 2012;14:1024-35 pubmed publisher
product information
Product No. :
A2859
Product Name :
Monoclonal Anti-ATG5 antibody produced in mouse
Host :
mouse
Conjugate :
unconjugated
Antibody Type :
monoclonal
Clone Number (if monoclonal) :
ATG5-18
Applications/Description :
Suitable for immunoprecipitation (IP); western blot: 2.5-5.0 μg/mL using whole extract of human K562 cells
Species Reactivity :
human, mouse, rat
Isotype :
IgG2a
Concentration :
~1.0 mg/mL
Storage :
Store at -20°C
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA