product summary
company name :
MilliporeSigma
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, FLUKA, Sigma-Aldrich, Novabiochem, Guava, Roche Applied Science
product type :
antibody
product name :
Monoclonal Anti-Actin, α-Smooth Muscle
catalog :
A2547
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
1A4
reactivity :
African green monkey, common marmoset, human, mouse, rat, dogs, chicken, bovine, pigs , fruit fly , domestic sheep, domestic rabbit, domestic horse, rhesus macaque
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, other, immunocytochemistry knockout validation
more info or order :
citations: 1642
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry knockout validation; mouse; loading ...; fig 2a
  • western blot; mouse; loading ...; fig 5a
Tomasek J, Haaksma C, Schwartz R, Howard E. Whole animal knockout of smooth muscle alpha-actin does not alter excisional wound healing or the fibroblast-to-myofibroblast transition. Wound Repair Regen. 2013;21:166-76 pubmed publisher
  • immunohistochemistry; mouse; 1:500; loading ...; fig 4d
Khasawneh R, Kist R, Queen R, Hussain R, Coxhead J, Schneider J, et al. Msx1 haploinsufficiency modifies the Pax9-deficient cardiovascular phenotype. BMC Dev Biol. 2021;21:14 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 1a
  • western blot; mouse; loading ...; fig 2a
  • western blot; rat; fig 3b
Sun W, Byon C, Kim D, Choi H, Park J, Joo S, et al. Renoprotective Effects of Maslinic Acid on Experimental Renal Fibrosis in Unilateral Ureteral Obstruction Model via Targeting MyD88. Front Pharmacol. 2021;12:708575 pubmed publisher
  • western blot; human; loading ...; fig 4b
  • western blot; mouse; loading ...; fig 2a
Fang L, Wang W, Chen J, Zuo A, Gao H, Yan T, et al. Osthole Attenuates Bleomycin-Induced Pulmonary Fibrosis by Modulating NADPH Oxidase 4-Derived Oxidative Stress in Mice. Oxid Med Cell Longev. 2021;2021:3309944 pubmed publisher
  • immunocytochemistry; human; 1:200; loading ...; fig 5b
  • immunocytochemistry; mouse; 1:200; loading ...; fig 1a
  • western blot; mouse; loading ...; fig 2c, 2j
Xi Y, Li Y, Xu P, Li S, Liu Z, Tung H, et al. The anti-fibrotic drug pirfenidone inhibits liver fibrosis by targeting the small oxidoreductase glutaredoxin-1. Sci Adv. 2021;7:eabg9241 pubmed publisher
  • immunohistochemistry - paraffin section; rat; loading ...; fig 4
  • western blot; rat; 1:1000; loading ...; fig 5d
Albino A, Zambom F, Foresto Neto O, Oliveira K, Ávila V, Arias S, et al. Renal Inflammation and Innate Immune Activation Underlie the Transition From Gentamicin-Induced Acute Kidney Injury to Renal Fibrosis. Front Physiol. 2021;12:606392 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:1000; loading ...; fig 1a
  • immunocytochemistry; human; 1:1000; loading ...; fig 7b
Zehender A, Li Y, Lin N, Stefanica A, Nüchel J, Chen C, et al. TGFβ promotes fibrosis by MYST1-dependent epigenetic regulation of autophagy. Nat Commun. 2021;12:4404 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig s2b
Zhang D, Huang J, Sun X, Chen H, Huang S, Yang J, et al. Targeting local lymphatics to ameliorate heterotopic ossification via FGFR3-BMPR1a pathway. Nat Commun. 2021;12:4391 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 1h
Goyette M, Elkholi I, Apcher C, Kuasne H, Rothlin C, Muller W, et al. Targeting Axl favors an antitumorigenic microenvironment that enhances immunotherapy responses by decreasing Hif-1α levels. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:1000; loading ...; fig 1a
Shani O, Raz Y, Monteran L, Scharff Y, Levi Galibov O, Megides O, et al. Evolution of fibroblasts in the lung metastatic microenvironment is driven by stage-specific transcriptional plasticity. elife. 2021;10: pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 2a
Zhang B, Lapenta K, Wang Q, Nam J, Chung D, Robert M, et al. Trefoil factor 2 secreted from damaged hepatocytes activates hepatic stellate cells to induce fibrogenesis. J Biol Chem. 2021;297:100887 pubmed publisher
  • western blot; human; loading ...; fig 5b
  • immunocytochemistry; mouse; 1:4000; loading ...; fig 5c
  • immunohistochemistry; mouse; 1:1000; loading ...; fig s3e
Jungwirth U, van Weverwijk A, Evans R, Jenkins L, Vicente D, Alexander J, et al. Impairment of a distinct cancer-associated fibroblast population limits tumour growth and metastasis. Nat Commun. 2021;12:3516 pubmed publisher
  • western blot; human; loading ...; fig 2a
Ostriker A, Xie Y, Chakraborty R, Sizer A, Bai Y, Ding M, et al. TET2 Protects Against Vascular Smooth Muscle Cell Apoptosis and Intimal Thickening in Transplant Vasculopathy. Circulation. 2021;144:455-470 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:25,000; loading ...; fig 6b
Barthet V, Brucoli M, Ladds M, Nössing C, Kiourtis C, Baudot A, et al. Autophagy suppresses the formation of hepatocyte-derived cancer-initiating ductular progenitor cells in the liver. Sci Adv. 2021;7: pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:50; loading ...; fig 1d
van Batenburg A, Kazemier K, Van Oosterhout M, van der Vis J, Grutters J, Goldschmeding R, et al. Telomere shortening and DNA damage in culprit cells of different types of progressive fibrosing interstitial lung disease. ERJ Open Res. 2021;7: pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 3.1 ug/ml; loading ...; fig 4b
Williams H, Wadey K, Frankow A, Blythe H, Forbes T, Johnson J, et al. Aneurysm severity is suppressed by deletion of CCN4. J Cell Commun Signal. 2021;15:421-432 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:1000; loading ...; fig 6c
Palau V, Nugraha B, Benito D, Pascual J, Emmert M, Hoerstrup S, et al. Both Specific Endothelial and Proximal Tubular Adam17 Deletion Protect against Diabetic Nephropathy. Int J Mol Sci. 2021;22: pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:1000; fig 6i
  • immunocytochemistry; mouse; 1:1000; fig 2g
Kemp S, Carpenter E, Steele N, Donahue K, Nwosu Z, Pacheco A, et al. Apolipoprotein E Promotes Immune Suppression in Pancreatic Cancer through NF-κB-Mediated Production of CXCL1. Cancer Res. 2021;81:4305-4318 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 6b
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig 2a
Flamini S, Sergeev P, Viana de Barros Z, Mello T, Biagioli M, Paglialunga M, et al. Glucocorticoid-induced leucine zipper regulates liver fibrosis by suppressing CCL2-mediated leukocyte recruitment. Cell Death Dis. 2021;12:421 pubmed publisher
  • immunocytochemistry; human; 1:1000; loading ...; fig 1c
Yamamura Y, Furuichi K, Murakawa Y, Hirabayashi S, Yoshihara M, Sako K, et al. Identification of candidate PAX2-regulated genes implicated in human kidney development. Sci Rep. 2021;11:9123 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig 1h
  • western blot; human; loading ...; fig 3b
O Hare M, Amarnani D, Whitmore H, An M, Marino C, Ramos L, et al. Targeting Runt-Related Transcription Factor 1 Prevents Pulmonary Fibrosis and Reduces Expression of Severe Acute Respiratory Syndrome Coronavirus 2 Host Mediators. Am J Pathol. 2021;191:1193-1208 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 5a
  • western blot; mouse; loading ...; fig 5b
Borges P, Waclawiak I, Georgii J, Fraga Junior V, Barros J, Lemos F, et al. Adenosine Diphosphate Improves Wound Healing in Diabetic Mice Through P2Y12 Receptor Activation. Front Immunol. 2021;12:651740 pubmed publisher
  • immunohistochemistry; human
Georgopoulou D, Callari M, Rueda O, Shea A, Martin A, Giovannetti A, et al. Landscapes of cellular phenotypic diversity in breast cancer xenografts and their impact on drug response. Nat Commun. 2021;12:1998 pubmed publisher
  • western blot; human; loading ...; fig 1a
Fell S, Wang Z, Blanchard A, Nanthakumar C, Griffin M. Transglutaminase 2: a novel therapeutic target for idiopathic pulmonary fibrosis using selective small molecule inhibitors. Amino Acids. 2021;53:205-217 pubmed publisher
  • western blot; human; 1:2000; loading ...; fig 1c
Ledein L, Leger B, Dees C, Beyer C, Distler A, Vettori S, et al. Translational engagement of lysophosphatidic acid receptor 1 in skin fibrosis: from dermal fibroblasts of patients with scleroderma to tight skin 1 mouse. Br J Pharmacol. 2020;177:4296-4309 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 1d
Lechertier T, Reynolds L, Kim H, Pedrosa A, Gómez Escudero J, Muñoz Félix J, et al. Pericyte FAK negatively regulates Gas6/Axl signalling to suppress tumour angiogenesis and tumour growth. Nat Commun. 2020;11:2810 pubmed publisher
  • immunocytochemistry; African green monkey; 1:1000; loading ...; fig 6a
  • immunocytochemistry; rhesus macaque; 1:1000; loading ...; fig 6a
Stauske M, Rodriguez Polo I, Haas W, Knorr D, Borchert T, Streckfuss Bömeke K, et al. Non-Human Primate iPSC Generation, Cultivation, and Cardiac Differentiation under Chemically Defined Conditions. Cells. 2020;9: pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:200; loading ...; fig 6f
Somerville T, Biffi G, Da ler Plenker J, Hur S, He X, Vance K, et al. Squamous trans-differentiation of pancreatic cancer cells promotes stromal inflammation. elife. 2020;9: pubmed publisher
  • immunohistochemistry; mouse; 5.0-7.5 ug/ml; loading ...; fig 2c
Di Gregoli K, Somerville M, Bianco R, Thomas A, Frankow A, Newby A, et al. Galectin-3 Identifies a Subset of Macrophages With a Potential Beneficial Role in Atherosclerosis. Arterioscler Thromb Vasc Biol. 2020;40:1491-1509 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 7
  • western blot; mouse; 1:250; loading ...; fig 7g, h
Gremlich S, Roth Kleiner M, Equey L, Fytianos K, Schittny J, Cremona T. Tenascin-C inactivation impacts lung structure and function beyond lung development. Sci Rep. 2020;10:5118 pubmed publisher
  • immunohistochemistry - paraffin section; domestic rabbit; 1:500; loading ...; fig 4a
Meier Bürgisser G, Evrova O, Calcagni M, Scalera C, Giovanoli P, Buschmann J. Impact of PDGF-BB on cellular distribution and extracellular matrix in the healing rabbit Achilles tendon three weeks post-operation. FEBS Open Bio. 2020;10:327-337 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig s8c, s9c
Mantani P, Dunér P, Ljungcrantz I, Nilsson J, Bjorkbacka H, Fredrikson G. ILC2 transfers to apolipoprotein E deficient mice reduce the lipid content of atherosclerotic lesions. BMC Immunol. 2019;20:47 pubmed publisher
  • immunohistochemistry; mouse; 1:200; loading ...; fig 4h, s3j, s6f
Yung T, Poon F, Liang M, Coquenlorge S, McGaugh E, Hui C, et al. Sufu- and Spop-mediated downregulation of Hedgehog signaling promotes beta cell differentiation through organ-specific niche signals. Nat Commun. 2019;10:4647 pubmed publisher
  • western blot; human; loading ...; fig 5c
Ren J, Smid M, Iaria J, Salvatori D, van Dam H, Zhu H, et al. Cancer-associated fibroblast-derived Gremlin 1 promotes breast cancer progression. Breast Cancer Res. 2019;21:109 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...
Cibi D, Mia M, Guna Shekeran S, Yun L, Sandireddy R, Gupta P, et al. Neural crest-specific deletion of Rbfox2 in mice leads to craniofacial abnormalities including cleft palate. elife. 2019;8: pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 4f
Wen H, Gao S, Wang Y, Ray M, Magnuson M, Wright C, et al. Myeloid cell-derived HB-EGF Drives Tissue Recovery After Pancreatitis. Cell Mol Gastroenterol Hepatol. 2019;: pubmed publisher
  • immunocytochemistry; human; 1:500; loading ...; fig 4c26
An S, Raju I, Surenkhuu B, Kwon J, Gulati S, Karaman M, et al. Neutrophil extracellular traps (NETs) contribute to pathological changes of ocular graft-vs.-host disease (oGVHD) dry eye: Implications for novel biomarkers and therapeutic strategies. Ocul Surf. 2019;: pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 6f
Farbehi N, Patrick R, Dorison A, Xaymardan M, Janbandhu V, Wystub Lis K, et al. Single-cell expression profiling reveals dynamic flux of cardiac stromal, vascular and immune cells in health and injury. elife. 2019;8: pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 3b
Wu W, Zhang W, Choi M, Zhao J, Gao P, Xue M, et al. Vascular smooth muscle-MAPK14 is required for neointimal hyperplasia by suppressing VSMC differentiation and inducing proliferation and inflammation. Redox Biol. 2019;22:101137 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:400; loading ...; fig s5c
Sahara M, Santoro F, Sohlmér J, Zhou C, Witman N, Leung C, et al. Population and Single-Cell Analysis of Human Cardiogenesis Reveals Unique LGR5 Ventricular Progenitors in Embryonic Outflow Tract. Dev Cell. 2019;48:475-490.e7 pubmed publisher
  • immunocytochemistry; human; fig 1i
Wimmer R, Leopoldi A, Aichinger M, Wick N, Hantusch B, Novatchkova M, et al. Human blood vessel organoids as a model of diabetic vasculopathy. Nature. 2019;565:505-510 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 2g
Mahmoud M, Evans I, Mehta V, Pellet Many C, Paliashvili K, Zachary I. Smooth muscle cell-specific knockout of neuropilin-1 impairs postnatal lung development and pathological vascular smooth muscle cell accumulation. Am J Physiol Cell Physiol. 2019;316:C424-C433 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig s4e
Quitterer U, Fu X, Pohl A, Bayoumy K, Langer A, AbdAlla S. Beta-Arrestin1 Prevents Preeclampsia by Downregulation of Mechanosensitive AT1-B2 Receptor Heteromers. Cell. 2019;176:318-333.e19 pubmed publisher
  • immunohistochemistry; human; 1:200; fig 1c
Weltner J, Balboa D, Katayama S, Bespalov M, Krjutskov K, Jouhilahti E, et al. Human pluripotent reprogramming with CRISPR activators. Nat Commun. 2018;9:2643 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig s4b
García Nores G, Ly C, Cuzzone D, Kataru R, Hespe G, Torrisi J, et al. CD4+ T cells are activated in regional lymph nodes and migrate to skin to initiate lymphedema. Nat Commun. 2018;9:1970 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:2500
Rickelt S, Hynes R. Antibodies and methods for immunohistochemistry of extracellular matrix proteins. Matrix Biol. 2018;71-72:10-27 pubmed publisher
  • western blot; human; 1:1000; fig 8a
Ho L, Skiba N, Ullmer C, Rao P. Lysophosphatidic Acid Induces ECM Production via Activation of the Mechanosensitive YAP/TAZ Transcriptional Pathway in Trabecular Meshwork Cells. Invest Ophthalmol Vis Sci. 2018;59:1969-1984 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 2c
  • immunocytochemistry; mouse; 1:200; loading ...; fig 5f
  • immunohistochemistry - frozen section; human; 1:200; loading ...; fig 11c
Fu X, Khalil H, Kanisicak O, Boyer J, Vagnozzi R, Maliken B, et al. Specialized fibroblast differentiated states underlie scar formation in the infarcted mouse heart. J Clin Invest. 2018;128:2127-2143 pubmed publisher
  • immunohistochemistry - paraffin section; rat; 1:600; loading ...; fig 8a
Nofi C, Bogatyryov Y, Dedkov E. Preservation of Functional Microvascular Bed Is Vital for Long-Term Survival of Cardiac Myocytes Within Large Transmural Post-Myocardial Infarction Scar. J Histochem Cytochem. 2017;:22155417741640 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 2d
Van T, Polykratis A, Straub B, Kondylis V, Papadopoulou N, Pasparakis M. Kinase-independent functions of RIPK1 regulate hepatocyte survival and liver carcinogenesis. J Clin Invest. 2017;127:2662-2677 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:2500; loading ...; fig s9
Xia H, Gilbertsen A, Herrera J, Racila E, Smith K, Peterson M, et al. Calcium-binding protein S100A4 confers mesenchymal progenitor cell fibrogenicity in idiopathic pulmonary fibrosis. J Clin Invest. 2017;127:2586-2597 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:250; fig 1a
Tian H, Ketova T, Hardy D, Xu X, Gao X, Zijlstra A, et al. Endoglin Mediates Vascular Maturation by Promoting Vascular Smooth Muscle Cell Migration and Spreading. Arterioscler Thromb Vasc Biol. 2017;37:1115-1126 pubmed publisher
  • western blot; human; loading ...; fig 4c
Li P, Wang Y, Mao X, Jiang Y, Liu J, Li J, et al. CRB3 downregulation confers breast cancer stem cell traits through TAZ/?-catenin. Oncogenesis. 2017;6:e322 pubmed publisher
  • immunocytochemistry; human; 1:200; fig s2d
Qi W, Keenan H, Li Q, Ishikado A, Kannt A, Sadowski T, et al. Pyruvate kinase M2 activation may protect against the progression of diabetic glomerular pathology and mitochondrial dysfunction. Nat Med. 2017;23:753-762 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 3c
  • western blot; human; 1:1000; fig 3b
Gerarduzzi C, Kumar R, Trivedi P, Ajay A, Iyer A, Boswell S, et al. Silencing SMOC2 ameliorates kidney fibrosis by inhibiting fibroblast to myofibroblast transformation. JCI Insight. 2017;2: pubmed publisher
  • western blot; human; loading ...; fig 2a
Menicacci B, Laurenzana A, Chillà A, Margheri F, Peppicelli S, Tanganelli E, et al. Chronic Resveratrol Treatment Inhibits MRC5 Fibroblast SASP-Related Protumoral Effects on Melanoma Cells. J Gerontol A Biol Sci Med Sci. 2017;72:1187-1195 pubmed publisher
  • immunohistochemistry; mouse; 1:2000; loading ...; fig 8i
Barry C, Ji E, Sharma H, Beukes L, Vilimas P, Degraaf Y, et al. Morphological and neurochemical differences in peptidergic nerve fibers of the mouse vagina. J Comp Neurol. 2017;525:2394-2410 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 5e
Arioka Y, Ito H, Hirata A, Semi K, Yamada Y, Seishima M. Behavior of leucine-rich repeat-containing G-protein coupled receptor 5-expressing cells in the reprogramming process. Stem Cell Res. 2017;20:1-9 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 3b
Schatton D, Pla Martín D, Marx M, Hansen H, Mourier A, Nemazanyy I, et al. CLUH regulates mitochondrial metabolism by controlling translation and decay of target mRNAs. J Cell Biol. 2017;216:675-693 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 2e
Lovric S, Gonçalves S, Gee H, Oskouian B, Srinivas H, Choi W, et al. Mutations in sphingosine-1-phosphate lyase cause nephrosis with ichthyosis and adrenal insufficiency. J Clin Invest. 2017;127:912-928 pubmed publisher
  • immunohistochemistry; rat; 1:100; fig 3a
  • western blot; rat; 1:2000; fig 2a
Shu D, Wojciechowski M, Lovicu F. Bone Morphogenetic Protein-7 Suppresses TGF?2-Induced Epithelial-Mesenchymal Transition in the Lens: Implications for Cataract Prevention. Invest Ophthalmol Vis Sci. 2017;58:781-796 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 2f
Xu J, Zhu S, Heng B, Dissanayaka W, Zhang C. TGF-?1-induced differentiation of SHED into functional smooth muscle cells. Stem Cell Res Ther. 2017;8:10 pubmed publisher
  • immunohistochemistry; human; 1:500; fig 2b
Gopal S, Veracini L, Grall D, Butori C, Schaub S, Audebert S, et al. Fibronectin-guided migration of carcinoma collectives. Nat Commun. 2017;8:14105 pubmed publisher
  • immunohistochemistry; mouse; 1:500; loading ...; fig 7a
Benito Jardón M, Klapproth S, Gimeno LLuch I, Petzold T, Bharadwaj M, Müller D, et al. The fibronectin synergy site re-enforces cell adhesion and mediates a crosstalk between integrin classes. elife. 2017;6: pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:500; loading ...; fig 1b
de Jong R, Paulin N, Lemnitzer P, Viola J, Winter C, Ferraro B, et al. Protective Aptitude of Annexin A1 in Arterial Neointima Formation in Atherosclerosis-Prone Mice-Brief Report. Arterioscler Thromb Vasc Biol. 2017;37:312-315 pubmed publisher
  • immunocytochemistry; human; 1:150; loading ...; fig s1j
Hosoya M, Fujioka M, Sone T, Okamoto S, Akamatsu W, Ukai H, et al. Cochlear Cell Modeling Using Disease-Specific iPSCs Unveils a Degenerative Phenotype and Suggests Treatments for Congenital Progressive Hearing Loss. Cell Rep. 2017;18:68-81 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 1d
Yang J, Savvatis K, Kang J, Fan P, Zhong H, Schwartz K, et al. Targeting LOXL2 for cardiac interstitial fibrosis and heart failure treatment. Nat Commun. 2016;7:13710 pubmed publisher
  • immunocytochemistry; mouse; 1:2000; fig 2a
Hofmann K, Fiedler S, Vierkotten S, Weber J, Klee S, Jia J, et al. Classical transient receptor potential 6 (TRPC6) channels support myofibroblast differentiation and development of experimental pulmonary fibrosis. Biochim Biophys Acta Mol Basis Dis. 2017;1863:560-568 pubmed publisher
  • immunocytochemistry; mouse; fig 6a
Tian S, Li C, Ran R, Chen S. Surfactant protein A deficiency exacerbates renal interstitial fibrosis following obstructive injury in mice. Biochim Biophys Acta Mol Basis Dis. 2017;1863:509-517 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 2c
Goreczny G, Ouderkirk Pecone J, Olson E, Krendel M, Turner C. Hic-5 remodeling of the stromal matrix promotes breast tumor progression. Oncogene. 2017;36:2693-2703 pubmed publisher
  • immunocytochemistry; human; 1:2000; fig 1
Herring A, Messana A, Bara A, Hazelbaker D, Eggan K, Barrett L. Generation of a TLE1 homozygous knockout human embryonic stem cell line using CRISPR-Cas9. Stem Cell Res. 2016;17:430-432 pubmed publisher
  • western blot; rat; 1:1000; fig 8
Keshri G, Gupta A, Yadav A, Sharma S, Singh S. Photobiomodulation with Pulsed and Continuous Wave Near-Infrared Laser (810 nm, Al-Ga-As) Augments Dermal Wound Healing in Immunosuppressed Rats. PLoS ONE. 2016;11:e0166705 pubmed publisher
  • immunohistochemistry; mouse; fig 5c
Grootaert M, Schrijvers D, Hermans M, Van Hoof V, De Meyer G, Martinet W. Caspase-3 Deletion Promotes Necrosis in Atherosclerotic Plaques of ApoE Knockout Mice. Oxid Med Cell Longev. 2016;2016:3087469 pubmed
  • immunohistochemistry - paraffin section; mouse; 1:400; loading ...; fig 4d
Sizemore G, Balakrishnan S, Hammer A, Thies K, Trimboli A, Wallace J, et al. Stromal PTEN inhibits the expansion of mammary epithelial stem cells through Jagged-1. Oncogene. 2017;36:2297-2308 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 5f
Han J, Bae J, Choi C, Choi S, Kang H, Jo E, et al. Autophagy induced by AXL receptor tyrosine kinase alleviates acute liver injury via inhibition of NLRP3 inflammasome activation in mice. Autophagy. 2016;12:2326-2343 pubmed
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 2d
Chiche A, Moumen M, Romagnoli M, Petit V, Lasla H, Jézéquel P, et al. p53 deficiency induces cancer stem cell pool expansion in a mouse model of triple-negative breast tumors. Oncogene. 2017;36:2355-2365 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; fig 7
Ren S, Luo Y, Chen H, Warburton D, Lam H, Wang L, et al. Inactivation of Tsc2 in Mesoderm-Derived Cells Causes Polycystic Kidney Lesions and Impairs Lung Alveolarization. Am J Pathol. 2016;186:3261-3272 pubmed publisher
  • immunohistochemistry; human; 1:200; loading ...; fig 4a
Nguyen H, Kirkton R, Bursac N. Engineering prokaryotic channels for control of mammalian tissue excitability. Nat Commun. 2016;7:13132 pubmed publisher
  • immunocytochemistry; human; fig 1b
Che D, Zhou T, Lan Y, Xie J, Gong H, Li C, et al. High glucose-induced epithelial-mesenchymal transition contributes to the upregulation of fibrogenic factors in retinal pigment epithelial cells. Int J Mol Med. 2016;38:1815-1822 pubmed publisher
  • immunohistochemistry - paraffin section; rat; 1:4000; loading ...; tbl 2
Ta M, Schwensen K, Foster S, Korgaonkar M, Ozimek Kulik J, Phillips J, et al. Effects of TORC1 Inhibition during the Early and Established Phases of Polycystic Kidney Disease. PLoS ONE. 2016;11:e0164193 pubmed publisher
  • immunohistochemistry; rat; 1:500; loading ...; fig 3c
  • western blot; rat; 1:500; loading ...; fig 1b
Gupta S, Zeglinski M, Rattan S, Landry N, Ghavami S, Wigle J, et al. Inhibition of autophagy inhibits the conversion of cardiac fibroblasts to cardiac myofibroblasts. Oncotarget. 2016;7:78516-78531 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 2h
  • immunocytochemistry; mouse; loading ...; fig 4c
Liu Y, Wang T, Zhang R, Fu W, Wang X, Wang F, et al. Calorie restriction protects against experimental abdominal aortic aneurysms in mice. J Exp Med. 2016;213:2473-2488 pubmed
  • western blot; human; 1:2000; fig 1
  • immunohistochemistry - paraffin section; mouse; 1:400; fig 5
  • western blot; mouse; 1:2000; fig s1
Chen P, Qin L, Li G, Tellides G, Simons M. Fibroblast growth factor (FGF) signaling regulates transforming growth factor beta (TGF?)-dependent smooth muscle cell phenotype modulation. Sci Rep. 2016;6:33407 pubmed publisher
  • western blot; human; fig 1
  • immunohistochemistry - paraffin section; mouse; fig 5
Sato N, Takasaka N, Yoshida M, Tsubouchi K, Minagawa S, Araya J, et al. Metformin attenuates lung fibrosis development via NOX4 suppression. Respir Res. 2016;17:107 pubmed publisher
  • western blot; human; loading ...; fig 5a
Jurek A, Genander M, Kundu P, Catchpole T, He X, Strååt K, et al. Eph receptor interclass cooperation is required for the regulation of cell proliferation. Exp Cell Res. 2016;348:10-22 pubmed publisher
  • western blot; mouse; 1:500; tbl 1
Görtz G, Moshkelgosha S, Jesenek C, Edelmann B, Horstmann M, Banga J, et al. Pathogenic Phenotype of Adipogenesis and Hyaluronan in Orbital Fibroblasts From Female Graves' Orbitopathy Mouse Model. Endocrinology. 2016;157:3771-3778 pubmed
  • immunohistochemistry - paraffin section; mouse; 1:200; fig 2
  • western blot; mouse; 1:5000; fig 3
Riascos Bernal D, Chinnasamy P, Cao L, Dunaway C, Valenta T, Basler K, et al. β-Catenin C-terminal signals suppress p53 and are essential for artery formation. Nat Commun. 2016;7:12389 pubmed publisher
  • immunocytochemistry; human; 1:900; loading ...; fig 1d
Medrano J, Rombaut C, Simon C, Pellicer A, Goossens E. Human spermatogonial stem cells display limited proliferation in vitro under mouse spermatogonial stem cell culture conditions. Fertil Steril. 2016;106:1539-1549.e8 pubmed publisher
  • western blot; human; 1:1000; fig s2
Kaukonen R, Mai A, Georgiadou M, Saari M, De Franceschi N, Betz T, et al. Normal stroma suppresses cancer cell proliferation via mechanosensitive regulation of JMJD1a-mediated transcription. Nat Commun. 2016;7:12237 pubmed publisher
  • immunohistochemistry - paraffin section; rat; 1:4000; loading ...; tbl 1
Ta M, Schwensen K, Liuwantara D, Huso D, Watnick T, Rangan G. Constitutive renal Rel/nuclear factor-?B expression in Lewis polycystic kidney disease rats. World J Nephrol. 2016;5:339-57 pubmed publisher
  • immunocytochemistry; mouse; 1:200; fig 3k
Dauleh S, Santeramo I, Fielding C, Ward K, Herrmann A, Murray P, et al. Characterisation of Cultured Mesothelial Cells Derived from the Murine Adult Omentum. PLoS ONE. 2016;11:e0158997 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 4l
Yu Q, Song W, Wang D, Zeng Y. Identification of blood vascular endothelial stem cells by the expression of protein C receptor. Cell Res. 2016;26:1079-1098 pubmed publisher
  • immunohistochemistry - paraffin section; rat; fig 3
Kim J, Hong S, Park C, Park J, Choi S, Woo S, et al. Intramyocardial Adipose-Derived Stem Cell Transplantation Increases Pericardial Fat with Recovery of Myocardial Function after Acute Myocardial Infarction. PLoS ONE. 2016;11:e0158067 pubmed publisher
  • immunohistochemistry; mouse; 1:200; fig 1
Barrionuevo F, Hurtado A, Kim G, Real F, Bakkali M, Kopp J, et al. Sox9 and Sox8 protect the adult testis from male-to-female genetic reprogramming and complete degeneration. elife. 2016;5: pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:1000; tbl 1
  • western blot; mouse; 1:1000; tbl 2
Muñoz Félix J, Pérez Roque L, Núñez Gómez E, Oujo B, Arevalo M, Ruiz Remolina L, et al. Overexpression of the short endoglin isoform reduces renal fibrosis and inflammation after unilateral ureteral obstruction. Biochim Biophys Acta. 2016;1862:1801-14 pubmed publisher
  • immunohistochemistry; mouse; 1:4000; fig 6
Lin W, Lim S, Yen T, Alison M. The Influence of Bone Marrow-Secreted IL-10 in a Mouse Model of Cerulein-Induced Pancreatic Fibrosis. Biomed Res Int. 2016;2016:4601532 pubmed publisher
  • immunohistochemistry - paraffin section; rat; 1:100; fig 3
Zaglia T, Di Bona A, Chioato T, Basso C, Ausoni S, Mongillo M. Optimized protocol for immunostaining of experimental GFP-expressing and human hearts. Histochem Cell Biol. 2016;146:407-19 pubmed publisher
  • western blot; human; fig 1
Kobayashi K, Araya J, Minagawa S, Hara H, Saito N, Kadota T, et al. Involvement of PARK2-Mediated Mitophagy in Idiopathic Pulmonary Fibrosis Pathogenesis. J Immunol. 2016;197:504-16 pubmed publisher
  • immunocytochemistry; mouse; 1:500; fig 1
Farrugia A, Calvo F. Cdc42 regulates Cdc42EP3 function in cancer-associated fibroblasts. Small Gtpases. 2017;8:49-57 pubmed publisher
  • western blot; rat; 1:1000; fig 1
Puchert M, Adams V, Linke A, Engele J. Evidence for the involvement of the CXCL12 system in the adaptation of skeletal muscles to physical exercise. Cell Signal. 2016;28:1205-15 pubmed publisher
  • immunohistochemistry; human; loading ...; fig 3d
Chesnokova V, Zonis S, Zhou C, Recouvreux M, Ben Shlomo A, Araki T, et al. Growth hormone is permissive for neoplastic colon growth. Proc Natl Acad Sci U S A. 2016;113:E3250-9 pubmed publisher
  • immunocytochemistry; human; 1:1000; fig 1
Momcilovic O, Sivapatham R, Oron T, Meyer M, Mooney S, Rao M, et al. Derivation, Characterization, and Neural Differentiation of Integration-Free Induced Pluripotent Stem Cell Lines from Parkinson's Disease Patients Carrying SNCA, LRRK2, PARK2, and GBA Mutations. PLoS ONE. 2016;11:e0154890 pubmed publisher
  • immunohistochemistry - frozen section; human; 1:400; fig 4
  • immunocytochemistry; human; 1:2000; fig 2
Chen P, Qin L, Li G, Tellides G, Simons M. Smooth muscle FGF/TGFβ cross talk regulates atherosclerosis progression. EMBO Mol Med. 2016;8:712-28 pubmed publisher
  • western blot; mouse; fig 5A
Chen L, DeWispelaere A, Dastvan F, Osborne W, Blechner C, Windhorst S, et al. Smooth Muscle-Alpha Actin Inhibits Vascular Smooth Muscle Cell Proliferation and Migration by Inhibiting Rac1 Activity. PLoS ONE. 2016;11:e0155726 pubmed publisher
  • immunocytochemistry; mouse; fig 2
Ramesh S, Singh A, Cibi D, Hausenloy D, Singh M. In Vitro Culture of Epicardial Cells From Mouse Embryonic Heart. J Vis Exp. 2016;: pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:500; loading ...; fig s6d
  • immunocytochemistry; mouse; 1:1000; loading ...; fig 1g
Miyawaki S, Kawamura Y, Oiwa Y, Shimizu A, Hachiya T, Bono H, et al. Tumour resistance in induced pluripotent stem cells derived from naked mole-rats. Nat Commun. 2016;7:11471 pubmed publisher
  • western blot; human; fig 3
Ding H, Xu Y, Gao D, Wang L. Glioma-associated oncogene homolog 1 promotes epithelial-mesenchymal transition in human renal tubular epithelial cell. Am J Transl Res. 2016;8:662-9 pubmed
  • immunocytochemistry; mouse; fig 1
Zhu D, Hadoke P, Wu J, Vesey A, Lerman D, Dweck M, et al. Ablation of the androgen receptor from vascular smooth muscle cells demonstrates a role for testosterone in vascular calcification. Sci Rep. 2016;6:24807 pubmed publisher
  • immunocytochemistry; mouse; 1:1000; fig 4
Waisbourd Zinman O, Koh H, Tsai S, Lavrut P, Dang C, Zhao X, et al. The toxin biliatresone causes mouse extrahepatic cholangiocyte damage and fibrosis through decreased glutathione and SOX17. Hepatology. 2016;64:880-93 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; fig 1
Wezel A, De Vries M, Maassen J, Kip P, Peters E, Karper J, et al. Deficiency of the TLR4 analogue RP105 aggravates vein graft disease by inducing a pro-inflammatory response. Sci Rep. 2016;6:24248 pubmed publisher
  • immunocytochemistry; human; fig 6
Månsson Broberg A, Rodin S, Bulatovic I, Ibarra C, Löfling M, Genead R, et al. Wnt/?-Catenin Stimulation and Laminins Support Cardiovascular Cell Progenitor Expansion from Human Fetal Cardiac Mesenchymal Stromal Cells. Stem Cell Reports. 2016;6:607-617 pubmed publisher
  • immunohistochemistry - paraffin section; human; fig 7
  • western blot; human; fig 3
Park S, Choi Y, Jung N, Yu Y, Ryu K, Kim H, et al. Myogenic differentiation potential of human tonsil-derived mesenchymal stem cells and their potential for use to promote skeletal muscle regeneration. Int J Mol Med. 2016;37:1209-20 pubmed publisher
  • immunohistochemistry; human; 1:100; fig 4d
Gruosso T, Mieulet V, Cardon M, Bourachot B, Kieffer Y, Devun F, et al. Chronic oxidative stress promotes H2AX protein degradation and enhances chemosensitivity in breast cancer patients. EMBO Mol Med. 2016;8:527-49 pubmed publisher
  • western blot; rat; 1:2000; loading ...; fig 1a
  • immunohistochemistry; human; 1:50,000; loading ...; fig 12
Djamali A, Wilson N, Sadowski E, Zha W, Niles D, Hafez O, et al. Nox2 and Cyclosporine-Induced Renal Hypoxia. Transplantation. 2016;100:1198-210 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:200; fig 1
Thakur A, Nigri J, Lac S, Leca J, Bressy C, Berthezene P, et al. TAp73 loss favors Smad-independent TGF-β signaling that drives EMT in pancreatic ductal adenocarcinoma. Cell Death Differ. 2016;23:1358-70 pubmed publisher
  • immunohistochemistry; mouse; fig 2
  • western blot; mouse; fig 3
Liu S, Wu C, Huang K, Wang C, Guan S, Chen L, et al. C/EBP homologous protein (CHOP) deficiency ameliorates renal fibrosis in unilateral ureteral obstructive kidney disease. Oncotarget. 2016;7:21900-12 pubmed publisher
  • immunohistochemistry; mouse; fig 8
Marneros A. Increased VEGF-A promotes multiple distinct aging diseases of the eye through shared pathomechanisms. EMBO Mol Med. 2016;8:208-31 pubmed publisher
  • immunohistochemistry; rat; 1:2500; loading ...; fig 4a
Rodionova K, Fiedler C, Guenther F, Grouzmann E, Neuhuber W, Fischer M, et al. Complex reinnervation pattern after unilateral renal denervation in rats. Am J Physiol Regul Integr Comp Physiol. 2016;310:R806-18 pubmed publisher
  • western blot; human; fig 4
Kim D, Helfman D. Loss of MLCK leads to disruption of cell-cell adhesion and invasive behavior of breast epithelial cells via increased expression of EGFR and ERK/JNK signaling. Oncogene. 2016;35:4495-508 pubmed publisher
  • immunocytochemistry; mouse; 1:500; loading ...; fig s1a
Scognamiglio R, Cabezas Wallscheid N, Thier M, Altamura S, Reyes A, Prendergast Ã, et al. Myc Depletion Induces a Pluripotent Dormant State Mimicking Diapause. Cell. 2016;164:668-80 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; fig 7
Jean Charles P, Zhang L, Wu J, Han S, Brian L, Freedman N, et al. Ubiquitin-specific Protease 20 Regulates the Reciprocal Functions of β-Arrestin2 in Toll-like Receptor 4-promoted Nuclear Factor κB (NFκB) Activation. J Biol Chem. 2016;291:7450-64 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:5000; fig 4
Allaire J, Roy S, Ouellet C, Lemieux Ã, Jones C, Paquet M, et al. Bmp signaling in colonic mesenchyme regulates stromal microenvironment and protects from polyposis initiation. Int J Cancer. 2016;138:2700-12 pubmed publisher
  • immunocytochemistry; domestic horse; 1:300; fig 1
Quattrocelli M, Giacomazzi G, Broeckx S, Ceelen L, Bolca S, Spaas J, et al. Equine-Induced Pluripotent Stem Cells Retain Lineage Commitment Toward Myogenic and Chondrogenic Fates. Stem Cell Reports. 2016;6:55-63 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:400; fig 1
Lai C, Wang K, Lee F, Tsai H, Ma C, Cheng T, et al. Toll-Like Receptor 4 Is Essential in the Development of Abdominal Aortic Aneurysm. PLoS ONE. 2016;11:e0146565 pubmed publisher
  • immunocytochemistry; mouse; 1:100; fig 3
Maimets M, Rocchi C, Bron R, Pringle S, Kuipers J, Giepmans B, et al. Long-Term In Vitro Expansion of Salivary Gland Stem Cells Driven by Wnt Signals. Stem Cell Reports. 2016;6:150-62 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:300; fig 4
Zahavi T, Lanton T, Divon M, Salmon A, Peretz T, Galun E, et al. Sorafenib treatment during partial hepatectomy reduces tumorgenesis in an inflammation-associated liver cancer model. Oncotarget. 2016;7:4860-70 pubmed publisher
  • immunocytochemistry; dogs; fig S1g
Loperfido M, Jarmin S, Dastidar S, Di Matteo M, Perini I, Moore M, et al. piggyBac transposons expressing full-length human dystrophin enable genetic correction of dystrophic mesoangioblasts. Nucleic Acids Res. 2016;44:744-60 pubmed publisher
  • immunocytochemistry; rat; 1:500; fig 1
Smith K, Zhou B, Avdulov S, Benyumov A, Peterson M, Liu Y, et al. Transforming Growth Factor-β1 Induced Epithelial Mesenchymal Transition is blocked by a chemical antagonist of translation factor eIF4E. Sci Rep. 2015;5:18233 pubmed publisher
  • immunohistochemistry; mouse; fig 4
Tarin C, Carril M, Martin Ventura J, Markuerkiaga I, Padro D, Llamas Granda P, et al. Targeted gold-coated iron oxide nanoparticles for CD163 detection in atherosclerosis by MRI. Sci Rep. 2015;5:17135 pubmed publisher
  • western blot; human; 2.5 ug/ml; loading ...; fig 4b
Leclercq A, Veillat V, Loriot S, Spuul P, Madonna F, Roques X, et al. A Methodology for Concomitant Isolation of Intimal and Adventitial Endothelial Cells from the Human Thoracic Aorta. PLoS ONE. 2015;10:e0143144 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:500; fig 2
Jung M, Ryu Y, Kang G. Investigation of the origin of stromal and endothelial cells at the desmoplastic interface in xenograft tumor in mice. Pathol Res Pract. 2015;211:925-30 pubmed publisher
  • immunohistochemistry - paraffin section; rat; 1:1000; fig 6
Hall A, Ashton S, Horner J, Wilson Z, Reens J, Richmond G, et al. PDGFR Inhibition Results in Pericyte Depletion and Hemorrhage into the Corpus Luteum of the Rat Ovary. Toxicol Pathol. 2016;44:98-111 pubmed publisher
  • western blot; mouse; 1:1000; fig 4a
Abshagen K, Senne M, Genz B, Thomas M, Vollmar B. Differential Effects of Axin2 Deficiency on the Fibrogenic and Regenerative Response in Livers of Bile Duct-Ligated Mice. Eur Surg Res. 2015;55:328-340 pubmed
  • immunohistochemistry - paraffin section; mouse; 1:500; loading ...; fig 4a
Baba I, Egi Y, Utsumi H, Kakimoto T, Suzuki K. Inhibitory effects of fasudil on renal interstitial fibrosis induced by unilateral ureteral obstruction. Mol Med Rep. 2015;12:8010-20 pubmed publisher
  • immunohistochemistry; mouse; 1:100; fig 8i-l
Manda K, Tripathi P, Hsi A, Ning J, Ruzinova M, Liapis H, et al. NFATc1 promotes prostate tumorigenesis and overcomes PTEN loss-induced senescence. Oncogene. 2016;35:3282-92 pubmed publisher
  • western blot; rat; 1:500; fig 7
Aoki K, Teshima Y, Kondo H, Saito S, Fukui A, Fukunaga N, et al. Role of Indoxyl Sulfate as a Predisposing Factor for Atrial Fibrillation in Renal Dysfunction. J Am Heart Assoc. 2015;4:e002023 pubmed publisher
  • western blot; human; 1:5000; fig 2
Xing M, Wang X, Palmai Pallag T, Shen H, Helleday T, Hickson I, et al. Acute MUS81 depletion leads to replication fork slowing and a constitutive DNA damage response. Oncotarget. 2015;6:37638-46 pubmed publisher
  • immunohistochemistry; mouse
Grootaert M, da Costa Martins P, Bitsch N, Pintelon I, De Meyer G, Martinet W, et al. Defective autophagy in vascular smooth muscle cells accelerates senescence and promotes neointima formation and atherogenesis. Autophagy. 2015;11:2014-2032 pubmed publisher
  • immunocytochemistry; human; fig 4
  • western blot; human; fig 4
Doldi V, Callari M, Giannoni E, D Aiuto F, Maffezzini M, Valdagni R, et al. Integrated gene and miRNA expression analysis of prostate cancer associated fibroblasts supports a prominent role for interleukin-6 in fibroblast activation. Oncotarget. 2015;6:31441-60 pubmed publisher
  • immunohistochemistry; mouse; 1:800
Forbes M, Thornhill B, Galarreta C, Chevalier R. A population of mitochondrion-rich cells in the pars recta of mouse kidney. Cell Tissue Res. 2016;363:791-803 pubmed publisher
  • western blot; mouse; 1:1000; fig 6f
Quijada P, Hariharan N, Cubillo J, Bala K, Emathinger J, Wang B, et al. Nuclear Calcium/Calmodulin-dependent Protein Kinase II Signaling Enhances Cardiac Progenitor Cell Survival and Cardiac Lineage Commitment. J Biol Chem. 2015;290:25411-26 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:500; fig s2
Chang C, Zhang M, Rajapakshe K, Coarfa C, Edwards D, Huang S, et al. Mammary Stem Cells and Tumor-Initiating Cells Are More Resistant to Apoptosis and Exhibit Increased DNA Repair Activity in Response to DNA Damage. Stem Cell Reports. 2015;5:378-91 pubmed publisher
  • immunocytochemistry; mouse
Fu Y, Huang C, Xu X, Gu H, Ye Y, Jiang C, et al. Direct reprogramming of mouse fibroblasts into cardiomyocytes with chemical cocktails. Cell Res. 2015;25:1013-24 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 1c
Manieri N, Mack M, Himmelrich M, Worthley D, Hanson E, Eckmann L, et al. Mucosally transplanted mesenchymal stem cells stimulate intestinal healing by promoting angiogenesis. J Clin Invest. 2015;125:3606-18 pubmed publisher
  • western blot; mouse; fig 1d
Fan Z, Hao C, Li M, Dai X, Qin H, Li J, et al. MKL1 is an epigenetic modulator of TGF-β induced fibrogenesis. Biochim Biophys Acta. 2015;1849:1219-28 pubmed publisher
  • other; mouse; 1:200; fig 2,3,5,6,7
  • western blot; mouse; 1:4000
Kramann R, Fleig S, Schneider R, Fabian S, DiRocco D, Maarouf O, et al. Pharmacological GLI2 inhibition prevents myofibroblast cell-cycle progression and reduces kidney fibrosis. J Clin Invest. 2015;125:2935-51 pubmed publisher
  • immunocytochemistry; human; 1:300; fig 5g
Birket M, Ribeiro M, Verkerk A, Ward D, Leitoguinho A, Den Hartogh S, et al. Expansion and patterning of cardiovascular progenitors derived from human pluripotent stem cells. Nat Biotechnol. 2015;33:970-9 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:50; fig 2
Malik I, Stange I, Martius G, Cameron S, Rave Fränk M, Hess C, et al. Role of PECAM-1 in radiation-induced liver inflammation. J Cell Mol Med. 2015;19:2441-52 pubmed publisher
  • immunohistochemistry; human; fig 5
Ruiz de Garibay G, Herranz C, Llorente A, Boni J, Serra Musach J, Mateo F, et al. Lymphangioleiomyomatosis Biomarkers Linked to Lung Metastatic Potential and Cell Stemness. PLoS ONE. 2015;10:e0132546 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:5000; fig 1
Grewal N, Franken R, Mulder B, Goumans M, Lindeman J, Jongbloed M, et al. Histopathology of aortic complications in bicuspid aortic valve versus Marfan syndrome: relevance for therapy?. Heart Vessels. 2016;31:795-806 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; fig 8a
  • immunocytochemistry; mouse; 1:100; loading ...; fig 1e
Singh N, Kotla S, Dyukova E, Traylor J, Orr A, Chernoff J, et al. Disruption of p21-activated kinase 1 gene diminishes atherosclerosis in apolipoprotein E-deficient mice. Nat Commun. 2015;6:7450 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:4000
Lokody I, Francis J, Gardiner J, Erler J, Swain A. Pten Regulates Epithelial Cytodifferentiation during Prostate Development. PLoS ONE. 2015;10:e0129470 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:200; fig s1
Ruozi G, Bortolotti F, Falcione A, Dal Ferro M, Ukovich L, Macedo A, et al. AAV-mediated in vivo functional selection of tissue-protective factors against ischaemia. Nat Commun. 2015;6:7388 pubmed publisher
  • immunohistochemistry - paraffin section; rat; 1:10,000; fig 7
Frenay A, Yazdani S, Boersema M, van der Graaf A, Waanders F, van den Born J, et al. Incomplete Restoration of Angiotensin II-Induced Renal Extracellular Matrix Deposition and Inflammation Despite Complete Functional Recovery in Rats. PLoS ONE. 2015;10:e0129732 pubmed publisher
  • western blot; human; fig 3
Yan M, Chu L, Qin B, Wang Z, Liu X, Jin C, et al. Regulation of NDR1 activity by PLK1 ensures proper spindle orientation in mitosis. Sci Rep. 2015;5:10449 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:400
Wei K, Díaz Trelles R, Liu Q, Diez Cuñado M, Scimia M, Cai W, et al. Developmental origin of age-related coronary artery disease. Cardiovasc Res. 2015;107:287-94 pubmed publisher
  • immunocytochemistry; rat; 1:1000
Pimenta M, Francisco R, Silva R, Porto C, Lazari M. Relaxin affects cell organization and early and late stages of spermatogenesis in a coculture of rat testicular cells. Andrology. 2015;3:772-86 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:500; fig 2
Louveau A, Smirnov I, Keyes T, Eccles J, Rouhani S, Peske J, et al. Structural and functional features of central nervous system lymphatic vessels. Nature. 2015;523:337-41 pubmed publisher
  • western blot; mouse; 1:2000; loading ...; fig 5c
Cushing L, Costinean S, Xu W, Jiang Z, Madden L, Kuang P, et al. Disruption of miR-29 Leads to Aberrant Differentiation of Smooth Muscle Cells Selectively Associated with Distal Lung Vasculature. PLoS Genet. 2015;11:e1005238 pubmed publisher
  • immunohistochemistry; rat; 1:50; fig 5
Matsunaga A, Harita Y, Shibagaki Y, Shimizu N, Shibuya K, Ono H, et al. Identification of 4-Trimethylaminobutyraldehyde Dehydrogenase (TMABA-DH) as a Candidate Serum Autoantibody Target for Kawasaki Disease. PLoS ONE. 2015;10:e0128189 pubmed publisher
  • immunohistochemistry - paraffin section; domestic horse; 1:500
Chevigny M, Guérin Montpetit K, Vargas A, Lefebvre Lavoie J, Lavoie J. Contribution of SRF, Elk-1, and myocardin to airway smooth muscle remodeling in heaves, an asthma-like disease of horses. Am J Physiol Lung Cell Mol Physiol. 2015;309:L37-45 pubmed publisher
  • flow cytometry; mouse; fig 1
Doni A, Musso T, Morone D, Bastone A, Zambelli V, Sironi M, et al. An acidic microenvironment sets the humoral pattern recognition molecule PTX3 in a tissue repair mode. J Exp Med. 2015;212:905-25 pubmed publisher
  • immunohistochemistry; mouse; 1:10,000; tbl 2, f5, s5, s6
Bernichtein S, Pigat N, Capiod T, Boutillon F, Verkarre V, Camparo P, et al. High milk consumption does not affect prostate tumor progression in two mouse models of benign and neoplastic lesions. PLoS ONE. 2015;10:e0125423 pubmed publisher
  • immunocytochemistry; human
  • western blot; human
Katanov C, Lerrer S, Liubomirski Y, Leider Trejo L, Meshel T, Bar J, et al. Regulation of the inflammatory profile of stromal cells in human breast cancer: prominent roles for TNF-? and the NF-?B pathway. Stem Cell Res Ther. 2015;6:87 pubmed publisher
  • western blot; rat; 1:1000; fig 1
Yongping M, Zhang X, Xuewei L, Fan W, Chen J, Zhang H, et al. Astragaloside prevents BDL-induced liver fibrosis through inhibition of notch signaling activation. J Ethnopharmacol. 2015;169:200-9 pubmed publisher
  • western blot; rat; fig 1
Lee H, Jeong H, Park S, Yoo W, Choi S, Choi K, et al. Fusion protein of retinol-binding protein and albumin domain III reduces liver fibrosis. EMBO Mol Med. 2015;7:819-30 pubmed publisher
  • western blot; human; fig 3b
Li Y, Drabsch Y, Pujuguet P, Ren J, van Laar T, Zhang L, et al. Genetic depletion and pharmacological targeting of αv integrin in breast cancer cells impairs metastasis in zebrafish and mouse xenograft models. Breast Cancer Res. 2015;17:28 pubmed publisher
  • western blot; human; 1:5000
Dayer C, Stamenkovic I. Recruitment of Matrix Metalloproteinase-9 (MMP-9) to the Fibroblast Cell Surface by Lysyl Hydroxylase 3 (LH3) Triggers Transforming Growth Factor-β (TGF-β) Activation and Fibroblast Differentiation. J Biol Chem. 2015;290:13763-78 pubmed publisher
  • immunohistochemistry - paraffin section; rat; 1:500
Maquigussa E, Arnoni C, Pereira L, Boim M. Calcitriol ameliorates renal damage in a pre-established proteinuria model. Mol Med Rep. 2015;12:1009-15 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 1a
  • flow cytometry; mouse; fig 2d
  • western blot; mouse; loading ...; fig 1c, 5c
Jia D, Duan F, Peng P, Sun L, Ruan Y, Gu J. Pyrroloquinoline-quinone suppresses liver fibrogenesis in mice. PLoS ONE. 2015;10:e0121939 pubmed publisher
  • immunocytochemistry; human; 1:100
Videla Richardson G, Garcia C, Roisman A, Slavutsky I, Fernandez Espinosa D, Romorini L, et al. Specific Preferences in Lineage Choice and Phenotypic Plasticity of Glioma Stem Cells Under BMP4 and Noggin Influence. Brain Pathol. 2016;26:43-61 pubmed publisher
  • other; human; loading ...; fig 6b
  • western blot; human; loading ...; fig 2b
Yamada A, Aki T, Unuma K, Funakoshi T, Uemura K. Paraquat induces epithelial-mesenchymal transition-like cellular response resulting in fibrogenesis and the prevention of apoptosis in human pulmonary epithelial cells. PLoS ONE. 2015;10:e0120192 pubmed publisher
  • immunocytochemistry; human; fig s1
  • western blot; human; fig s1
Kawada M, Inoue H, Ohba S, Yoshida J, Masuda T, Yamasaki M, et al. Stromal cells positively and negatively modulate the growth of cancer cells: stimulation via the PGE2-TNFα-IL-6 pathway and inhibition via secreted GAPDH-E-cadherin interaction. PLoS ONE. 2015;10:e0119415 pubmed publisher
  • immunocytochemistry; common marmoset; 1:750; fig 3
  • immunohistochemistry; common marmoset; 1:1000; fig 3
Debowski K, Warthemann R, Lentes J, Salinas Riester G, Dressel R, Langenstroth D, et al. Non-viral generation of marmoset monkey iPS cells by a six-factor-in-one-vector approach. PLoS ONE. 2015;10:e0118424 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:100; fig 3
Kitamura M, Nishino T, Obata Y, Oka S, Abe S, Muta K, et al. The kampo medicine Daikenchuto inhibits peritoneal fibrosis in mice. Biol Pharm Bull. 2015;38:193-200 pubmed publisher
  • immunocytochemistry; dogs; 1:400
Liu M, Flanagan T, Lu C, French A, Argyle D, Corcoran B. Culture and characterisation of canine mitral valve interstitial and endothelial cells. Vet J. 2015;204:32-9 pubmed publisher
  • immunohistochemistry; mouse; 1:15000; fig s3
Kijani S, Yrlid U, Heyden M, Levin M, Borén J, Fogelstrand P. Filter-Dense Multicolor Microscopy. PLoS ONE. 2015;10:e0119499 pubmed publisher
  • immunohistochemistry - paraffin section; rat
Otsuki S, Sawada H, Yodoya N, Shinohara T, Kato T, Ohashi H, et al. Potential contribution of phenotypically modulated smooth muscle cells and related inflammation in the development of experimental obstructive pulmonary vasculopathy in rats. PLoS ONE. 2015;10:e0118655 pubmed publisher
  • immunocytochemistry; human; 1:500
Fritz A, Adil M, Mao S, Schaffer D. cAMP and EPAC Signaling Functionally Replace OCT4 During Induced Pluripotent Stem Cell Reprogramming. Mol Ther. 2015;23:952-963 pubmed publisher
  • immunohistochemistry; human; 1:10000
Gallos G, Yocum G, Siviski M, Yim P, Fu X, Poe M, et al. Selective targeting of the α5-subunit of GABAA receptors relaxes airway smooth muscle and inhibits cellular calcium handling. Am J Physiol Lung Cell Mol Physiol. 2015;308:L931-42 pubmed publisher
  • immunocytochemistry; human
Afzal M, Strande J. Generation of induced pluripotent stem cells from muscular dystrophy patients: efficient integration-free reprogramming of urine derived cells. J Vis Exp. 2015;:52032 pubmed publisher
  • western blot; rat; fig 4
Yang L, Hu J, Hao H, Yin Z, Liu G, Zou X. Sodium tanshinone IIA sulfonate attenuates the transforming growth factor-β1-induced differentiation of atrial fibroblasts into myofibroblasts in vitro. Int J Mol Med. 2015;35:1026-32 pubmed publisher
  • flow cytometry; human
Shapero K, Wylie Sears J, Levine R, Mayer J, Bischoff J. Reciprocal interactions between mitral valve endothelial and interstitial cells reduce endothelial-to-mesenchymal transition and myofibroblastic activation. J Mol Cell Cardiol. 2015;80:175-85 pubmed publisher
  • western blot; mouse; fig 1A
  • western blot; human; fig 2A
Bárcena C, Stefanovic M, Tutusaus A, Martinez Nieto G, Martinez L, García Ruiz C, et al. Angiogenin secretion from hepatoma cells activates hepatic stellate cells to amplify a self-sustained cycle promoting liver cancer. Sci Rep. 2015;5:7916 pubmed publisher
  • western blot; mouse
Malhotra R, Burke M, Martyn T, Shakartzi H, Thayer T, O Rourke C, et al. Inhibition of bone morphogenetic protein signal transduction prevents the medial vascular calcification associated with matrix Gla protein deficiency. PLoS ONE. 2015;10:e0117098 pubmed publisher
  • immunohistochemistry - frozen section; human; 1:1000; fig 2
Coutelle O, Schiffmann L, Liwschitz M, Brunold M, Goede V, Hallek M, et al. Dual targeting of Angiopoetin-2 and VEGF potentiates effective vascular normalisation without inducing empty basement membrane sleeves in xenograft tumours. Br J Cancer. 2015;112:495-503 pubmed publisher
  • immunocytochemistry; mouse; 1:400
Kalwa H, Storch U, Demleitner J, Fiedler S, Mayer T, Kannler M, et al. Phospholipase C epsilon (PLCε) induced TRPC6 activation: a common but redundant mechanism in primary podocytes. J Cell Physiol. 2015;230:1389-99 pubmed publisher
  • immunohistochemistry - paraffin section; rat; 1:4000; tbl 2
Ta M, Rao P, Korgaonkar M, Foster S, Peduto A, Harris D, et al. Pyrrolidine dithiocarbamate reduces the progression of total kidney volume and cyst enlargement in experimental polycystic kidney disease. Physiol Rep. 2014;2: pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:30000
Vogels R, Vlenterie M, Versleijen Jonkers Y, Ruijter E, Bekers E, Verdijk M, et al. Solitary fibrous tumor - clinicopathologic, immunohistochemical and molecular analysis of 28 cases. Diagn Pathol. 2014;9:224 pubmed publisher
  • immunohistochemistry; mouse; fig s2
O Hara L, McInnes K, Simitsidellis I, Morgan S, Atanassova N, Slowikowska Hilczer J, et al. Autocrine androgen action is essential for Leydig cell maturation and function, and protects against late-onset Leydig cell apoptosis in both mice and men. FASEB J. 2015;29:894-910 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:1600
Barnes M, McMullen M, Roychowdhury S, Madhun N, Niese K, Olman M, et al. Macrophage migration inhibitory factor is required for recruitment of scar-associated macrophages during liver fibrosis. J Leukoc Biol. 2015;97:161-9 pubmed publisher
  • immunocytochemistry; mouse; 1:300; fig 4
Macías D, Fernández Agüera M, Bonilla Henao V, López Barneo J. Deletion of the von Hippel-Lindau gene causes sympathoadrenal cell death and impairs chemoreceptor-mediated adaptation to hypoxia. EMBO Mol Med. 2014;6:1577-92 pubmed publisher
  • immunohistochemistry; mouse; fig 1
Taniguchi T, Asano Y, Akamata K, Noda S, Takahashi T, Ichimura Y, et al. Fibrosis, vascular activation, and immune abnormalities resembling systemic sclerosis in bleomycin-treated Fli-1-haploinsufficient mice. Arthritis Rheumatol. 2015;67:517-26 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:1000
Moreira R, Velz T, Alves N, Gesche V, Malischewski A, Schmitz Rode T, et al. Tissue-engineered heart valve with a tubular leaflet design for minimally invasive transcatheter implantation. Tissue Eng Part C Methods. 2015;21:530-40 pubmed publisher
  • immunohistochemistry - paraffin section; mouse
Venalis P, Kumánovics G, Schulze Koops H, Distler A, Dees C, Zerr P, et al. Cardiomyopathy in murine models of systemic sclerosis. Arthritis Rheumatol. 2015;67:508-16 pubmed publisher
  • immunocytochemistry; mouse; 1:1000; fig 3
Lim A, Shin K, Zhao C, Kawano S, Beachy P. Spatially restricted Hedgehog signalling regulates HGF-induced branching of the adult prostate. Nat Cell Biol. 2014;16:1135-45 pubmed publisher
  • immunohistochemistry; mouse; 1:200; loading ...; fig 5c
Delgado Olguín P, Dang L, He D, Thomas S, Chi L, Sukonnik T, et al. Ezh2-mediated repression of a transcriptional pathway upstream of Mmp9 maintains integrity of the developing vasculature. Development. 2014;141:4610-7 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:1000
Kunasegaran K, Ho V, Chang T, De Silva D, Bakker M, Christoffels V, et al. Transcriptional repressor Tbx3 is required for the hormone-sensing cell lineage in mammary epithelium. PLoS ONE. 2014;9:e110191 pubmed publisher
  • immunohistochemistry; mouse; 1:1000; fig 5
  • western blot; mouse; 1:1000; fig 5
Oujo B, Muñoz Félix J, Arévalo M, Núñez Gómez E, Pérez Roque L, Pericacho M, et al. L-Endoglin overexpression increases renal fibrosis after unilateral ureteral obstruction. PLoS ONE. 2014;9:e110365 pubmed publisher
  • immunocytochemistry; human; 1:400
Kim W, Barron D, San Martin R, Chan K, Tran L, Yang F, et al. RUNX1 is essential for mesenchymal stem cell proliferation and myofibroblast differentiation. Proc Natl Acad Sci U S A. 2014;111:16389-94 pubmed publisher
  • western blot; human; fig 1
Cai X, Dai Z, Reeves R, Caballero Benítez A, Duran K, Delrow J, et al. Autonomous stimulation of cancer cell plasticity by the human NKG2D lymphocyte receptor coexpressed with its ligands on cancer cells. PLoS ONE. 2014;9:e108942 pubmed publisher
  • immunohistochemistry - paraffin section; human; fig 2
  • immunohistochemistry; mouse; fig 3
  • western blot; mouse; fig 2
Liang N, Zhang C, Dill P, Panasyuk G, Pion D, Koka V, et al. Regulation of YAP by mTOR and autophagy reveals a therapeutic target of tuberous sclerosis complex. J Exp Med. 2014;211:2249-63 pubmed publisher
  • immunohistochemistry - frozen section; mouse
Morioka T, Sakabe M, Ioka T, Iguchi T, Mizuta K, Hattammaru M, et al. An important role of endothelial hairy-related transcription factors in mouse vascular development. Genesis. 2014;52:897-906 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:500
Pickup M, Hover L, Polikowsky E, Chytil A, Gorska A, Novitskiy S, et al. BMPR2 loss in fibroblasts promotes mammary carcinoma metastasis via increased inflammation. Mol Oncol. 2015;9:179-91 pubmed publisher
  • western blot; human
Li J, Liu J, Li P, Mao X, Li W, Yang J, et al. Loss of LKB1 disrupts breast epithelial cell polarity and promotes breast cancer metastasis and invasion. J Exp Clin Cancer Res. 2014;33:70 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:1000
Miller L, Lincoln J. Isolation of murine valve endothelial cells. J Vis Exp. 2014;: pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:500
Vannucchi M, Traini C, Guasti D, Del Popolo G, Faussone Pellegrini M. Telocytes subtypes in human urinary bladder. J Cell Mol Med. 2014;18:2000-8 pubmed publisher
  • immunohistochemistry - frozen section; rat; 1:1000; fig 1
Traini C, Faussone Pellegrini M, Evangelista S, Mazzaferro K, Cipriani G, Santicioli P, et al. Inner and outer portions of colonic circular muscle: ultrastructural and immunohistochemical changes in rat chronically treated with otilonium bromide. PLoS ONE. 2014;9:e103237 pubmed publisher
  • immunohistochemistry; human; fig 6
Aga M, Bradley J, Wanchu R, Yang Y, Acott T, Keller K. Differential effects of caveolin-1 and -2 knockdown on aqueous outflow and altered extracellular matrix turnover in caveolin-silenced trabecular meshwork cells. Invest Ophthalmol Vis Sci. 2014;55:5497-509 pubmed publisher
  • immunohistochemistry - frozen section; human; fig 2
Calabro S, Maczurek A, Morgan A, Tu T, Wen V, Yee C, et al. Hepatocyte produced matrix metalloproteinases are regulated by CD147 in liver fibrogenesis. PLoS ONE. 2014;9:e90571 pubmed publisher
  • western blot; human
Romero A, Caceres M, Arancibia R, Silva D, Couve E, Martinez C, et al. Cigarette smoke condensate inhibits collagen gel contraction and prostaglandin E2 production in human gingival fibroblasts. J Periodontal Res. 2015;50:371-9 pubmed publisher
  • immunohistochemistry; human; 1:500
  • immunohistochemistry - frozen section; pigs
Le Provost G, Pullar C. ?2-adrenoceptor activation modulates skin wound healing processes to reduce scarring. J Invest Dermatol. 2015;135:279-88 pubmed publisher
  • immunocytochemistry; human
Carlessi L, Fusar Poli E, Bechi G, Mantegazza M, Pascucci B, Narciso L, et al. Functional and molecular defects of hiPSC-derived neurons from patients with ATM deficiency. Cell Death Dis. 2014;5:e1342 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:500
Owens P, Pickup M, Novitskiy S, Giltnane J, Gorska A, Hopkins C, et al. Inhibition of BMP signaling suppresses metastasis in mammary cancer. Oncogene. 2015;34:2437-49 pubmed publisher
  • immunohistochemistry; human; 1:1000
Jabari S, da Silveira A, de Oliveira E, Quint K, Wirries A, Neuhuber W, et al. Mucosal layers and related nerve fibres in non-chagasic and chagasic human colon--a quantitative immunohistochemical study. Cell Tissue Res. 2014;358:75-83 pubmed publisher
  • immunohistochemistry - paraffin section; rat; 1:20,000; fig 3
Wagenaar G, Sengers R, Laghmani E, Chen X, Lindeboom M, Roks A, et al. Angiotensin II type 2 receptor ligand PD123319 attenuates hyperoxia-induced lung and heart injury at a low dose in newborn rats. Am J Physiol Lung Cell Mol Physiol. 2014;307:L261-72 pubmed publisher
  • flow cytometry; human; 1:400
  • immunocytochemistry; human; 1:400
Karow M, Schichor C, Beckervordersandforth R, Berninger B. Lineage-reprogramming of pericyte-derived cells of the adult human brain into induced neurons. J Vis Exp. 2014;: pubmed publisher
  • western blot; mouse; 1:500
Gotha L, Lim S, Osherov A, Wolff R, Qiang B, Erlich I, et al. Heparan sulfate side chains have a critical role in the inhibitory effects of perlecan on vascular smooth muscle cell response to arterial injury. Am J Physiol Heart Circ Physiol. 2014;307:H337-45 pubmed publisher
  • immunocytochemistry; domestic rabbit; 1:500
Jiang Y, Kou Z, Wu T, An W, Zhou R, Wang H, et al. Xist deficiency and disorders of X-inactivation in rabbit embryonic stem cells can be rescued by transcription-factor-mediated conversion. Stem Cells Dev. 2014;23:2283-96 pubmed publisher
  • immunohistochemistry; mouse
Wang J, Eguchi K, Matsumoto S, Fujiu K, Komuro I, Nagai R, et al. The ?-3 polyunsaturated fatty acid, eicosapentaenoic acid, attenuates abdominal aortic aneurysm development via suppression of tissue remodeling. PLoS ONE. 2014;9:e96286 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:10000
Zhang J, Hatakeyama J, Eto K, Abe S. Reconstruction of a seminiferous tubule-like structure in a 3 dimensional culture system of re-aggregated mouse neonatal testicular cells within a collagen matrix. Gen Comp Endocrinol. 2014;205:121-32 pubmed publisher
  • immunohistochemistry - paraffin section; mouse
Li Y, Pan J, Wei C, Chen J, Liu Y, Liu J, et al. LIM homeodomain transcription factor Isl1 directs normal pyloric development by targeting Gata3. BMC Biol. 2014;12:25 pubmed publisher
  • immunohistochemistry - paraffin section; domestic sheep; 1:1000
  • immunocytochemistry; domestic sheep; 1:400
Moreira R, Gesche V, Hurtado Aguilar L, Schmitz Rode T, Frese J, Jockenhoevel S, et al. TexMi: development of tissue-engineered textile-reinforced mitral valve prosthesis. Tissue Eng Part C Methods. 2014;20:741-8 pubmed publisher
  • western blot; mouse; 1:1000
Swärd K, Albinsson S, Rippe C. Arterial dysfunction but maintained systemic blood pressure in cavin-1-deficient mice. PLoS ONE. 2014;9:e92428 pubmed publisher
  • immunocytochemistry; human; 1:250
  • immunohistochemistry; human; 1:250
Balk Møller E, Kim J, Hopkinson B, Timmermans Wielenga V, Petersen O, Villadsen R. A marker of endocrine receptor-positive cells, CEACAM6, is shared by two major classes of breast cancer: luminal and HER2-enriched. Am J Pathol. 2014;184:1198-208 pubmed publisher
  • immunohistochemistry; mouse
Kaenel P, Hahnewald S, Wotzkow C, Strange R, Andres A. Overexpression of EphB4 in the mammary epithelium shifts the differentiation pathway of progenitor cells and promotes branching activity and vascularization. Dev Growth Differ. 2014;56:255-75 pubmed publisher
  • immunocytochemistry; mouse; 1:500
Mäkelä J, Toppari J, Rivero Muller A, Ventelä S. Reconstruction of mouse testicular cellular microenvironments in long-term seminiferous tubule culture. PLoS ONE. 2014;9:e90088 pubmed publisher
  • immunohistochemistry; mouse
Namba F, Go H, Murphy J, La P, Yang G, Sengupta S, et al. Expression level and subcellular localization of heme oxygenase-1 modulates its cytoprotective properties in response to lung injury: a mouse model. PLoS ONE. 2014;9:e90936 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:5000
Grewal N, Gittenberger de Groot A, Poelmann R, Klautz R, Lindeman J, Goumans M, et al. Ascending aorta dilation in association with bicuspid aortic valve: a maturation defect of the aortic wall. J Thorac Cardiovasc Surg. 2014;148:1583-90 pubmed publisher
  • immunohistochemistry; mouse; 1:200
Son A, Sheleg M, Cooper M, Sun Y, Kleiman N, Zhou R. Formation of persistent hyperplastic primary vitreous in ephrin-A5-/- mice. Invest Ophthalmol Vis Sci. 2014;55:1594-606 pubmed publisher
  • western blot; fruit fly ; fig 1
Cuartero S, Fresán U, Reina O, Planet E, Espinàs M. Ibf1 and Ibf2 are novel CP190-interacting proteins required for insulator function. EMBO J. 2014;33:637-47 pubmed publisher
  • immunohistochemistry; mouse
Sastre C, Fernández Laso V, Madrigal Matute J, Munoz Garcia B, Moreno J, Pastor Vargas C, et al. Genetic deletion or TWEAK blocking antibody administration reduce atherosclerosis and enhance plaque stability in mice. J Cell Mol Med. 2014;18:721-34 pubmed publisher
  • immunohistochemistry; mouse
Yoshida T, Yamashita M, Horimai C, Hayashi M. Deletion of Krüppel-like factor 4 in endothelial and hematopoietic cells enhances neointimal formation following vascular injury. J Am Heart Assoc. 2014;3:e000622 pubmed publisher
  • immunohistochemistry - paraffin section; mouse
Redmond E, Liu W, Hamm K, Hatch E, Cahill P, Morrow D. Perivascular delivery of Notch 1 siRNA inhibits injury-induced arterial remodeling. PLoS ONE. 2014;9:e84122 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:1000
Chang T, Kunasegaran K, Tarulli G, De Silva D, Voorhoeve P, Pietersen A. New insights into lineage restriction of mammary gland epithelium using parity-identified mammary epithelial cells. Breast Cancer Res. 2014;16:R1 pubmed publisher
  • western blot; mouse
Toko H, Hariharan N, Konstandin M, Ormachea L, McGregor M, Gude N, et al. Differential regulation of cellular senescence and differentiation by prolyl isomerase Pin1 in cardiac progenitor cells. J Biol Chem. 2014;289:5348-56 pubmed publisher
  • immunohistochemistry - frozen section; human
Holzer L, Cor A, Holzer G. Expression of gap junction proteins connexins 26, 30, and 43 in Dupuytren's disease. Acta Orthop. 2014;85:97-101 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:400
  • immunohistochemistry; mouse; 1:400
Nakayama A, Nakayama M, Turner C, Höing S, Lepore J, Adams R. Ephrin-B2 controls PDGFR? internalization and signaling. Genes Dev. 2013;27:2576-89 pubmed publisher
  • immunohistochemistry - paraffin section; human
Pérot G, Mendiboure J, Brouste V, Velasco V, Terrier P, Bonvalot S, et al. Smooth muscle differentiation identifies two classes of poorly differentiated pleomorphic sarcomas with distinct outcome. Mod Pathol. 2014;27:840-50 pubmed publisher
  • immunohistochemistry; mouse; 1:2000
Kerr G, Young J, Horvay K, Abud H, Loveland K. Regulated Wnt/beta-catenin signaling sustains adult spermatogenesis in mice. Biol Reprod. 2014;90:3 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:500
Lafkas D, Rodilla V, Huyghe M, Mourao L, Kiaris H, Fre S. Notch3 marks clonogenic mammary luminal progenitor cells in vivo. J Cell Biol. 2013;203:47-56 pubmed publisher
  • immunohistochemistry; mouse; fig 1
Cagnet S, Faraldo M, Kreft M, Sonnenberg A, Raymond K, Glukhova M. Signaling events mediated by ?3?1 integrin are essential for mammary tumorigenesis. Oncogene. 2014;33:4286-95 pubmed publisher
  • immunocytochemistry; human; 1:1000
  • immunohistochemistry; human; 1:1000
Kossack N, Terwort N, Wistuba J, Ehmcke J, Schlatt S, Scholer H, et al. A combined approach facilitates the reliable detection of human spermatogonia in vitro. Hum Reprod. 2013;28:3012-25 pubmed publisher
  • immunohistochemistry; human; 1:4000
  • immunohistochemistry; mouse; 1:4000
Burger N, Haak M, de Bakker B, Al Shaibani Z, de Groot C, Christoffels V, et al. Systematic analysis of the development of the ductus venosus in wild type mouse and human embryos. Early Hum Dev. 2013;89:1067-73 pubmed publisher
  • immunohistochemistry; mouse
Yang J, Zeini M, Lin C, Lin C, Xiong Y, Shang C, et al. Epicardial calcineurin-NFAT signals through Smad2 to direct coronary smooth muscle cell and arterial wall development. Cardiovasc Res. 2014;101:120-9 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:1000
Orecchioni S, Gregato G, Martin Padura I, Reggiani F, Braidotti P, Mancuso P, et al. Complementary populations of human adipose CD34+ progenitor cells promote growth, angiogenesis, and metastasis of breast cancer. Cancer Res. 2013;73:5880-91 pubmed publisher
  • immunocytochemistry; mouse; 1:600
Stewart C, Wang Y, Bonilla Claudio M, Martin J, Gonzalez G, Taketo M, et al. CTNNB1 in mesenchyme regulates epithelial cell differentiation during Müllerian duct and postnatal uterine development. Mol Endocrinol. 2013;27:1442-54 pubmed publisher
  • immunohistochemistry - paraffin section; human
Martino Echarri E, Fernández Rodríguez R, Rodríguez Baena F, Barrientos Duran A, Torres Collado A, Plaza Calonge M, et al. Contribution of ADAMTS1 as a tumor suppressor gene in human breast carcinoma. Linking its tumor inhibitory properties to its proteolytic activity on nidogen-1 and nidogen-2. Int J Cancer. 2013;133:2315-24 pubmed publisher
  • immunohistochemistry; mouse; 1:200
Tripathi P, Wang Y, Coussens M, Manda K, Casey A, Lin C, et al. Activation of NFAT signaling establishes a tumorigenic microenvironment through cell autonomous and non-cell autonomous mechanisms. Oncogene. 2014;33:1840-9 pubmed publisher
  • western blot; rat; 1:7500
Ciceri P, Elli F, Brenna I, Volpi E, Brancaccio D, Cozzolino M. The calcimimetic calindol prevents high phosphate-induced vascular calcification by upregulating matrix GLA protein. Nephron Exp Nephrol. 2012;122:75-82 pubmed publisher
  • immunohistochemistry - paraffin section; human
Jones G, Bown M, Gretarsdottir S, Romaine S, Helgadottir A, Yu G, et al. A sequence variant associated with sortilin-1 (SORT1) on 1p13.3 is independently associated with abdominal aortic aneurysm. Hum Mol Genet. 2013;22:2941-7 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:500
Yu D, Ware C, Waterland R, Zhang J, Chen M, Gadkari M, et al. Developmentally programmed 3' CpG island methylation confers tissue- and cell-type-specific transcriptional activation. Mol Cell Biol. 2013;33:1845-58 pubmed publisher
  • immunohistochemistry; human; 15 ug/mL
Brereton M, Wareing M, Jones R, Greenwood S. Characterisation of K+ channels in human fetoplacental vascular smooth muscle cells. PLoS ONE. 2013;8:e57451 pubmed publisher
  • western blot; rat; 1:7500
Ciceri P, Elli F, Brenna I, Volpi E, Romagnoli S, Tosi D, et al. Lanthanum prevents high phosphate-induced vascular calcification by preserving vascular smooth muscle lineage markers. Calcif Tissue Int. 2013;92:521-30 pubmed publisher
  • immunocytochemistry; rat; 1:500
Ho W, Davis A, Chadha P, Greenwood I. Effective contractile response to voltage-gated Na+ channels revealed by a channel activator. Am J Physiol Cell Physiol. 2013;304:C739-47 pubmed publisher
  • western blot; mouse; fig 1
Steenhard B, Vanacore R, Friedman D, Zelenchuk A, Stroganova L, Isom K, et al. Upregulated expression of integrin ?1 in mesangial cells and integrin ?3 and vimentin in podocytes of Col4a3-null (Alport) mice. PLoS ONE. 2012;7:e50745 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:3000
Hoss M, Saric T, Denecke B, Peinkofer G, Bovi M, Groll J, et al. Expansion and differentiation of germline-derived pluripotent stem cells on biomaterials. Tissue Eng Part A. 2013;19:1067-80 pubmed publisher
  • flow cytometry; human
Bouacida A, Rosset P, Trichet V, Guilloton F, Espagnolle N, Cordonier T, et al. Pericyte-like progenitors show high immaturity and engraftment potential as compared with mesenchymal stem cells. PLoS ONE. 2012;7:e48648 pubmed publisher
  • western blot; chicken; 1:10000
Kosla J, Dvorak M, Cermák V. Molecular analysis of the TGF-beta controlled gene expression program in chicken embryo dermal myofibroblasts. Gene. 2013;513:90-100 pubmed publisher
  • western blot; mouse; fig 2
Rahman A, Ekman M, Shakirova Y, Andersson K, Morgelin M, Erjefalt J, et al. Late onset vascular dysfunction in the R6/1 model of Huntington's disease. Eur J Pharmacol. 2013;698:345-53 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:3000
  • western blot; mouse; 1:10000
Harmelink C, Peng Y, Debenedittis P, Chen H, Shou W, Jiao K. Myocardial Mycn is essential for mouse ventricular wall morphogenesis. Dev Biol. 2013;373:53-63 pubmed publisher
  • immunohistochemistry - paraffin section; human
  • western blot; human
Cigna N, Farrokhi Moshai E, Brayer S, Marchal Sommé J, Wemeau Stervinou L, Fabre A, et al. The hedgehog system machinery controls transforming growth factor-?-dependent myofibroblastic differentiation in humans: involvement in idiopathic pulmonary fibrosis. Am J Pathol. 2012;181:2126-37 pubmed publisher
  • immunocytochemistry; mouse; fig s2b
Tondeleir D, Lambrechts A, Muller M, Jonckheere V, Doll T, Vandamme D, et al. Cells lacking ?-actin are genetically reprogrammed and maintain conditional migratory capacity. Mol Cell Proteomics. 2012;11:255-71 pubmed publisher
  • immunohistochemistry; human; 1:5000
Yamada Y, Yamamoto H, Ohishi Y, Nishiyama K, Fukuhara M, Saitou T, et al. Sclerosing variant of perivascular epithelioid cell tumor in the female genital organs. Pathol Int. 2011;61:768-72 pubmed publisher
  • immunohistochemistry - paraffin section; human; fig 3
von Toerne C, Schmidt C, Adams J, Kiss E, Bedke J, Porubsky S, et al. Wnt pathway regulation in chronic renal allograft damage. Am J Transplant. 2009;9:2223-39 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:600
Yamazato Y, Ferreira A, Hong K, Sriramula S, Francis J, Yamazato M, et al. Prevention of pulmonary hypertension by Angiotensin-converting enzyme 2 gene transfer. Hypertension. 2009;54:365-71 pubmed publisher
  • immunohistochemistry - frozen section; rat; 1:1,000
Lennerz J, Rühle V, Ceppa E, Neuhuber W, Bunnett N, Grady E, et al. Calcitonin receptor-like receptor (CLR), receptor activity-modifying protein 1 (RAMP1), and calcitonin gene-related peptide (CGRP) immunoreactivity in the rat trigeminovascular system: differences between peripheral and central CGRP receptor distribu. J Comp Neurol. 2008;507:1277-99 pubmed publisher
Rodriguez Polo I, Mißbach S, Petkov S, Mattern F, Maierhofer A, Grządzielewska I, et al. A piggyBac-based platform for genome editing and clonal rhesus macaque iPSC line derivation. Sci Rep. 2021;11:15439 pubmed publisher
Wang W, Tang Y, Xiao Q, Lee W, Cheng B, Niu Z, et al. Stromal induction of BRD4 phosphorylation Results in Chromatin Remodeling and BET inhibitor Resistance in Colorectal Cancer. Nat Commun. 2021;12:4441 pubmed publisher
Júnior D, Hausen M, Asami J, Higa A, Leite F, Mambrini G, et al. A New Dermal Substitute Containing Polyvinyl Alcohol with Silver Nanoparticles and Collagen with Hyaluronic Acid: In Vitro and In Vivo Approaches. Antibiotics (Basel). 2021;10: pubmed publisher
Molnar A, Lakat T, Hosszú A, Szebeni B, Balogh A, Orfi L, et al. Lyophilization and homogenization of biological samples improves reproducibility and reduces standard deviation in molecular biology techniques. Amino Acids. 2021;53:917-928 pubmed publisher
Landry N, Rattan S, Filomeno K, Meier T, Meier S, Foran S, et al. SKI activates the Hippo pathway via LIMD1 to inhibit cardiac fibroblast activation. Basic Res Cardiol. 2021;116:25 pubmed publisher
Tang W, Li Y, Li A, Bronner M. Clonal analysis and dynamic imaging identify multipotency of individual Gallus gallus caudal hindbrain neural crest cells toward cardiac and enteric fates. Nat Commun. 2021;12:1894 pubmed publisher
Steele N, Biffi G, Kemp S, Zhang Y, Drouillard D, Syu L, et al. Inhibition of Hedgehog Signaling Alters Fibroblast Composition in Pancreatic Cancer. Clin Cancer Res. 2021;: pubmed publisher
Chen Y, Chen H, Fan H, Tung Y, Kuo C, Tu M, et al. Anti-Inflammatory, Antioxidant, and Antifibrotic Effects of Kefir Peptides on Salt-Induced Renal Vascular Damage and Dysfunction in Aged Stroke-Prone Spontaneously Hypertensive Rats. Antioxidants (Basel). 2020;9: pubmed publisher
Gao P, GAO P, Choi M, Chegireddy K, Slivano O, Zhao J, et al. Transcriptome analysis of mouse aortae reveals multiple novel pathways regulated by aging. Aging (Albany NY). 2020;12:15603-15623 pubmed publisher
Itami Y, Miyake M, Ohnishi S, Tatsumi Y, Gotoh D, Hori S, et al. Disabled Homolog 2 (DAB2) Protein in Tumor Microenvironment Correlates with Aggressive Phenotype in Human Urothelial Carcinoma of the Bladder. Diagnostics (Basel). 2020;10: pubmed publisher
Grepin R, Guyot M, Dumond A, Durivault J, Ambrosetti D, Roussel J, et al. The combination of bevacizumab/Avastin and erlotinib/Tarceva is relevant for the treatment of metastatic renal cell carcinoma: the role of a synonymous mutation of the EGFR receptor. Theranostics. 2020;10:1107-1121 pubmed publisher
Tseng V, Ni K, Allawzi A, Prohaska C, Hernandez Lagunas L, Elajaili H, et al. Extracellular Superoxide Dismutase Regulates Early Vascular Hyaluronan Remodeling in Hypoxic Pulmonary Hypertension. Sci Rep. 2020;10:280 pubmed publisher
Quan Y, Park W, Jin J, Kim W, Park S, Kang K. Sirtuin 3 Activation by Honokiol Decreases Unilateral Ureteral Obstruction-Induced Renal Inflammation and Fibrosis via Regulation of Mitochondrial Dynamics and the Renal NF-κBTGF-β1/Smad Signaling Pathway. Int J Mol Sci. 2020;21: pubmed publisher
Bernard M, Yang B, Migneault F, Turgeon J, Dieudé M, Olivier M, et al. Autophagy drives fibroblast senescence through MTORC2 regulation. Autophagy. 2020;:1-13 pubmed publisher
To K, Gui P, Li M, Zawieja S, Castorena Gonzalez J, Davis M. T-type, but not L-type, voltage-gated calcium channels are dispensable for lymphatic pacemaking and spontaneous contractions. Sci Rep. 2020;10:70 pubmed publisher
Metelli A, Wu B, Riesenberg B, Guglietta S, Huck J, Mills C, et al. Thrombin contributes to cancer immune evasion via proteolysis of platelet-bound GARP to activate LTGF-β. Sci Transl Med. 2020;12: pubmed publisher
Tibaldi E, Brocca A, Sticca A, Gola E, Pizzi M, Bordin L, et al. Fam20C-mediated phosphorylation of osteopontin is critical for its secretion but dispensable for its action as a cytokine in the activation of hepatic stellate cells in liver fibrogenesis. FASEB J. 2020;34:1122-1135 pubmed publisher
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product information
Product No. :
A2547
Product Name :
Monoclonal Anti-Actin, α-Smooth Muscle
Host :
mouse
Conjugate :
unconjugated
Antibody Type :
monoclonal
Clone Number (if monoclonal) :
1A4
Applications/Description :
Suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections) using smooth muscle cells; western blot using smooth muscle cells; indirect immunofluorescence: 1:400 using blood vessels in sections of human appendix; immunohistochemistr
Species Reactivity :
human, frog, sheep, chicken, goat, bovine, rat, guinea pig, mouse, canine, rabbit, snake
Isotype :
IgG2a
Storage :
Store at -20°C
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA