Monoclonal Anti-Alkaline Phosphatase, Human Placental-Agarose antibody produced in mouse

product summary

company name :

MilliporeSigma

other brands :

FLUKA, Sigma-Aldrich, Roche Applied Science

product type :

antibody

product name :

Monoclonal Anti-Alkaline Phosphatase, Human Placental-Agarose antibody produced in mouse

catalog :

A2080

clonality :

monoclonal

host :

mouse

conjugate :

agarose

clone name :

8B6

reactivity :

hamsters, human

application :

immunoprecipitation

more info or order :

MilliporeSigma product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
immunoprecipitation; hamsters	Tashima Y, Stanley P. Antibodies that detect O-linked ?-D-N-acetylglucosamine on the extracellular domain of cell surface glycoproteins. J Biol Chem. 2014;289:11132-42 pubmed publisher
	Müller R, Jenny A, Stanley P. The EGF repeat-specific O-GlcNAc-transferase Eogt interacts with notch signaling and pyrimidine metabolism pathways in Drosophila. PLoS ONE. 2013;8:e62835 pubmed publisher

product information

cat no :

A2080

brand :

SIGMA

name :

Monoclonal Anti-Alkaline Phosphatase, Human Placental-Agarose antibody produced in mouse

prod type :

Chemical

name suffix :

clone 8B6, purified immunoglobulin, PBS suspension

storage :

2-8C

storage temp :

2-8C

physical form :

Suspension in 0.1 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

shipped in :

wet ice

syns :

Monoclonal Anti-Alkaline Phosphatase, Human Placental; Anti-Phosphatase, Alkaline, Human Placental; PLAP

mdl no :

MFCD00162106

unspsc code :

12352203

wgk :

form :

PBS suspension

antibody form :

purified immunoglobulin

application(s) :

immunoprecipitation: suitable

species reactivity :

human

immunogen :

Human epidermoid carcinoma cell line expressing placental alkaline phosphatase.

clone :

8B6

application :

Anti-Alkaline phosphatase human placental antibody coupled to agarose was packed in a column and used to purify alkaline-phosphatase conjugated FGF receptor out of conditioned media from COS-7 cells. Column was washed with 1M NaCl.1; Cultured pancreatic islet cells were fixed with cold 4% paraformaldhyde at room temperature for 10 minutes and incubated with mouse monoclonal anti-Alkaline Phosphatase, Human Placental antibody at 1:500. Mouse monoclonal anti-Alkaline Phosphatase, Human Placental antibody was used to detect a unique molecular probe that consisted of the FGFR extracellular domain expressed as a chimera with human PLAP.

more info or order :

MilliporeSigma product webpage