This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
antibody
product name :
Anti-HA High Affinity
catalog :
11867431001
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
3F10
reactivity :
human, mouse, rat, brewer's yeast, Xenopus laevis
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, EMSA, chromatin immunoprecipitation
citations: 60
Published Application/Species/Sample/DilutionReference
  • western blot; brewer's yeast; fig 3a
Teixeira V, Johnsen L, Martínez Montañés F, Grippa A, Búxo L, Idrissi F, et al. Regulation of lipid droplets by metabolically controlled Ldo isoforms. J Cell Biol. 2018;217:127-138 pubmed publisher
  • western blot; brewer's yeast; 1:4000
Bolognesi B, Lorenzo Gotor N, Dhar R, Cirillo D, Baldrighi M, Tartaglia G, et al. A Concentration-Dependent Liquid Phase Separation Can Cause Toxicity upon Increased Protein Expression. Cell Rep. 2016;16:222-231 pubmed publisher
  • western blot; human; 1:2000; fig 1
  • western blot; rat; 1:2000; fig 3
  • immunocytochemistry; mouse; 1:1000; fig s5
Fusté N, Fernández Hernández R, Cemeli T, Mirantes C, Pedraza N, Rafel M, et al. Cytoplasmic cyclin D1 regulates cell invasion and metastasis through the phosphorylation of paxillin. Nat Commun. 2016;7:11581 pubmed publisher
  • western blot; human
Li R, Liao G, Nirujogi R, Pinto S, Shaw P, Huang T, et al. Phosphoproteomic Profiling Reveals Epstein-Barr Virus Protein Kinase Integration of DNA Damage Response and Mitotic Signaling. PLoS Pathog. 2015;11:e1005346 pubmed publisher
  • immunoprecipitation; mouse; fig 6c
Van Maldegem F, Maslen S, Johnson C, Chandra A, Ganesh K, Skehel M, et al. CTNNBL1 facilitates the association of CWC15 with CDC5L and is required to maintain the abundance of the Prp19 spliceosomal complex. Nucleic Acids Res. 2015;43:7058-69 pubmed publisher
  • immunoprecipitation; mouse; fig 4
  • western blot; mouse; fig 4
Bittencourt D, Lee B, Gao L, Gerke D, Stallcup M. Role of distinct surfaces of the G9a ankyrin repeat domain in histone and DNA methylation during embryonic stem cell self-renewal and differentiation. Epigenetics Chromatin. 2014;7:27 pubmed publisher
  • western blot; mouse; 1:1,000
Shiba Fukushima K, Inoshita T, Hattori N, Imai Y. Lysine 63-linked polyubiquitination is dispensable for Parkin-mediated mitophagy. J Biol Chem. 2014;289:33131-6 pubmed publisher
  • immunocytochemistry; human; 1:800
Deng Z, Matsuda K, Tanikawa C, Lin J, Furukawa Y, Hamamoto R, et al. Late Cornified Envelope Group I, a novel target of p53, regulates PRMT5 activity. Neoplasia. 2014;16:656-64 pubmed publisher
  • immunoprecipitation; human
Yanagi T, Krajewska M, Matsuzawa S, Reed J. PCTAIRE1 phosphorylates p27 and regulates mitosis in cancer cells. Cancer Res. 2014;74:5795-807 pubmed publisher
  • immunoprecipitation; mouse
  • immunocytochemistry; mouse
  • western blot; mouse
  • immunoprecipitation; human
  • immunocytochemistry; human
  • western blot; human
Ro S, Semple I, Park H, Park H, Park H, Kim M, et al. Sestrin2 promotes Unc-51-like kinase 1 mediated phosphorylation of p62/sequestosome-1. FEBS J. 2014;281:3816-27 pubmed publisher
  • western blot; human
Potu H, Peterson L, Pal A, Verhaegen M, Cao J, Talpaz M, et al. Usp5 links suppression of p53 and FAS levels in melanoma to the BRAF pathway. Oncotarget. 2014;5:5559-69 pubmed
  • immunoprecipitation; human
Davis C, Zhu W, Gibson C, Bowman Kirigin J, Sorensen L, Ling J, et al. ARF6 inhibition stabilizes the vasculature and enhances survival during endotoxic shock. J Immunol. 2014;192:6045-52 pubmed publisher
  • western blot; human; fig 1
Aida K, Hayashi H, Inamura K, Mizuno T, Sugiyama Y. Differential roles of ubiquitination in the degradation mechanism of cell surface-resident bile salt export pump and multidrug resistance-associated protein 2. Mol Pharmacol. 2014;85:482-91 pubmed publisher
  • western blot; human
Mücke K, Paulus C, Bernhardt K, Gerrer K, Schön K, Fink A, et al. Human cytomegalovirus major immediate early 1 protein targets host chromosomes by docking to the acidic pocket on the nucleosome surface. J Virol. 2014;88:1228-48 pubmed publisher
  • immunoprecipitation; human
Yasuda Inoue M, Kuroki M, Ariumi Y. Distinct DDX DEAD-box RNA helicases cooperate to modulate the HIV-1 Rev function. Biochem Biophys Res Commun. 2013;434:803-8 pubmed publisher
  • immunocytochemistry; human
Vogt D, Camus G, Herker E, Webster B, Tsou C, Greene W, et al. Lipid droplet-binding protein TIP47 regulates hepatitis C Virus RNA replication through interaction with the viral NS5A protein. PLoS Pathog. 2013;9:e1003302 pubmed publisher
  • immunoprecipitation; Xenopus laevis
Singh A, Winterbottom E, Ji Y, Hwang Y, Daar I. Abelson interactor 1 (ABI1) and its interaction with Wiskott-Aldrich syndrome protein (wasp) are critical for proper eye formation in Xenopus embryos. J Biol Chem. 2013;288:14135-46 pubmed publisher
  • immunoprecipitation; human
  • western blot; human; fig 5c
Wilkie Grantham R, Matsuzawa S, Reed J. Novel phosphorylation and ubiquitination sites regulate reactive oxygen species-dependent degradation of anti-apoptotic c-FLIP protein. J Biol Chem. 2013;288:12777-90 pubmed publisher
  • immunoprecipitation; human
  • immunocytochemistry; human
  • western blot; human
Matsuzaki H, Fujimoto T, Tanaka M, Shirasawa S. Tespa1 is a novel component of mitochondria-associated endoplasmic reticulum membranes and affects mitochondrial calcium flux. Biochem Biophys Res Commun. 2013;433:322-6 pubmed publisher
  • western blot; mouse; fig 6e
Schael S, Nüchel J, Muller S, Petermann P, Kormann J, Pérez Otaño I, et al. Casein kinase 2 phosphorylation of protein kinase C and casein kinase 2 substrate in neurons (PACSIN) 1 protein regulates neuronal spine formation. J Biol Chem. 2013;288:9303-12 pubmed publisher
  • immunocytochemistry; human; 1:1000
Aivar P, Fernandez Orth J, Gomis Pèrez C, Alberdi A, Alaimo A, Rodriguez M, et al. Surface expression and subunit specific control of steady protein levels by the Kv7.2 helix A-B linker. PLoS ONE. 2012;7:e47263 pubmed publisher
  • immunoprecipitation; human
  • western blot; human; fig 2
Katoh H, Okamoto T, Fukuhara T, Kambara H, Morita E, Mori Y, et al. Japanese encephalitis virus core protein inhibits stress granule formation through an interaction with Caprin-1 and facilitates viral propagation. J Virol. 2013;87:489-502 pubmed publisher
  • chromatin immunoprecipitation; mouse
  • western blot; mouse; 1:2000
Wallmen B, Schrempp M, Hecht A. Intrinsic properties of Tcf1 and Tcf4 splice variants determine cell-type-specific Wnt/?-catenin target gene expression. Nucleic Acids Res. 2012;40:9455-69 pubmed publisher
  • chromatin immunoprecipitation; human
Hintermair C, Heidemann M, Koch F, Descostes N, Gut M, Gut I, et al. Threonine-4 of mammalian RNA polymerase II CTD is targeted by Polo-like kinase 3 and required for transcriptional elongation. EMBO J. 2012;31:2784-97 pubmed publisher
  • immunoprecipitation; human
  • western blot; human
Pandithage R, Lilischkis R, Harting K, Wolf A, Jedamzik B, Lüscher Firzlaff J, et al. The regulation of SIRT2 function by cyclin-dependent kinases affects cell motility. J Cell Biol. 2008;180:915-29 pubmed publisher
Sheinboim D, Maza I, Dror I, Parikh S, Krupalnik V, Bell R, et al. OCT4 impedes cell fate redirection by the melanocyte lineage master regulator MITF in mouse ESCs. Nat Commun. 2017;8:1022 pubmed publisher
Wang S, Stanika R, Wang X, Hagen J, Kennedy M, Obermair G, et al. Densin-180 Controls the Trafficking and Signaling of L-Type Voltage-Gated Cav1.2 Ca2+ Channels at Excitatory Synapses. J Neurosci. 2017;37:4679-4691 pubmed publisher
Cho C, Lee K, Chen W, Wang C, Chang Y, Huang H, et al. MST3 promotes proliferation and tumorigenicity through the VAV2/Rac1 signal axis in breast cancer. Oncotarget. 2016;7:14586-604 pubmed publisher
Subramanian V, MacQueen A, Vader G, Shinohara M, Sanchez A, Borde V, et al. Chromosome Synapsis Alleviates Mek1-Dependent Suppression of Meiotic DNA Repair. PLoS Biol. 2016;14:e1002369 pubmed publisher
Yan D, Perrimon N. spenito is required for sex determination in Drosophila melanogaster. Proc Natl Acad Sci U S A. 2015;112:11606-11 pubmed publisher
Fimiani C, Goina E, Mallamaci A. Upregulating endogenous genes by an RNA-programmable artificial transactivator. Nucleic Acids Res. 2015;43:7850-64 pubmed publisher
Miyazaki T, Lin T, Ito K, Lee C, Stopfer M. A gustatory second-order neuron that connects sucrose-sensitive primary neurons and a distinct region of the gnathal ganglion in the Drosophila brain. J Neurogenet. 2015;29:144-55 pubmed publisher
Moriyama T, Sangel P, Yamaguchi H, Obuse C, Miyamoto Y, Oka M, et al. Identification and characterization of a nuclear localization signal of TRIM28 that overlaps with the HP1 box. Biochem Biophys Res Commun. 2015;462:201-7 pubmed publisher
Naito T, Takatsu H, Miyano R, Takada N, Nakayama K, Shin H. Phospholipid Flippase ATP10A Translocates Phosphatidylcholine and Is Involved in Plasma Membrane Dynamics. J Biol Chem. 2015;290:15004-17 pubmed publisher
Tatebayashi K, Yamamoto K, Nagoya M, Takayama T, Nishimura A, Sakurai M, et al. Osmosensing and scaffolding functions of the oligomeric four-transmembrane domain osmosensor Sho1. Nat Commun. 2015;6:6975 pubmed publisher
Linzen U, Lilischkis R, Pandithage R, Schilling B, Ullius A, Lüscher Firzlaff J, et al. ING5 is phosphorylated by CDK2 and controls cell proliferation independently of p53. PLoS ONE. 2015;10:e0123736 pubmed publisher
Raju D, Schonauer S, Hamzeh H, FLYNN K, Bradke F, Vom Dorp K, et al. Accumulation of glucosylceramide in the absence of the beta-glucosidase GBA2 alters cytoskeletal dynamics. PLoS Genet. 2015;11:e1005063 pubmed publisher
Padmanabhan R, Taneyhill L. Cadherin-6B undergoes macropinocytosis and clathrin-mediated endocytosis during cranial neural crest cell EMT. J Cell Sci. 2015;128:1773-86 pubmed publisher
Yanagi T, Shi R, Aza Blanc P, Reed J, Matsuzawa S. PCTAIRE1-knockdown sensitizes cancer cells to TNF family cytokines. PLoS ONE. 2015;10:e0119404 pubmed publisher
Masuda K, Chiyoda T, Sugiyama N, Segura Cabrera A, Kabe Y, Ueki A, et al. LATS1 and LATS2 phosphorylate CDC26 to modulate assembly of the tetratricopeptide repeat subcomplex of APC/C. PLoS ONE. 2015;10:e0118662 pubmed publisher
Ikeda T, Hirata S, Takamatsu K, Haruta M, Tsukamoto H, Ito T, et al. Suppression of Th1-mediated autoimmunity by embryonic stem cell-derived dendritic cells. PLoS ONE. 2014;9:e115198 pubmed publisher
Furriols M, Casanova J. Germline and somatic vitelline proteins colocalize in aggregates in the follicular epithelium of Drosophila ovaries. Fly (Austin). 2014;8:113-9 pubmed publisher
Lee J, Peng Y, Lin W, Parrish J. Coordinate control of terminal dendrite patterning and dynamics by the membrane protein Raw. Development. 2015;142:162-73 pubmed publisher
Ay A, Holland J, Sperlea A, Devakanmalai G, Knierer S, Sangervasi S, et al. Spatial gradients of protein-level time delays set the pace of the traveling segmentation clock waves. Development. 2014;141:4158-67 pubmed publisher
Chigwechokha P, Komatsu M, Itakura T, Shiozaki K. Nile Tilapia Neu3 sialidases: molecular cloning, functional characterization and expression in Oreochromis niloticus. Gene. 2014;552:155-64 pubmed publisher
Cohen M, King C, Dikeakos J, Mymryk J. Functional analysis of the C-terminal region of human adenovirus E1A reveals a misidentified nuclear localization signal. Virology. 2014;468-470:238-43 pubmed publisher
Boros J, Arnoult N, Stroobant V, Collet J, Decottignies A. Polycomb repressive complex 2 and H3K27me3 cooperate with H3K9 methylation to maintain heterochromatin protein 1? at chromatin. Mol Cell Biol. 2014;34:3662-74 pubmed publisher
Warring S, Dou Z, Carruthers V, McFadden G, van Dooren G. Characterization of the chloroquine resistance transporter homologue in Toxoplasma gondii. Eukaryot Cell. 2014;13:1360-70 pubmed publisher
Goodwin E, Motamedi N, Lipovsky A, Fernández Busnadiego R, DiMaio D. Expression of DNAJB12 or DNAJB14 causes coordinate invasion of the nucleus by membranes associated with a novel nuclear pore structure. PLoS ONE. 2014;9:e94322 pubmed publisher
Duval N, Daubas P, Bourcier de Carbon C, St Cloment C, Tinevez J, Lopes M, et al. Msx1 and Msx2 act as essential activators of Atoh1 expression in the murine spinal cord. Development. 2014;141:1726-36 pubmed publisher
Brenker C, Zhou Y, Müller A, Echeverry F, Trötschel C, Poetsch A, et al. The Ca2+-activated K+ current of human sperm is mediated by Slo3. elife. 2014;3:e01438 pubmed publisher
Liu Z, Fang J, Dearman J, Zhang L, Zuo J. In vivo generation of immature inner hair cells in neonatal mouse cochleae by ectopic Atoh1 expression. PLoS ONE. 2014;9:e89377 pubmed publisher
Okada T, Nitta T, Kaji K, Takashima A, Oda H, Tamehiro N, et al. Differential function of Themis CABIT domains during T cell development. PLoS ONE. 2014;9:e89115 pubmed publisher
Gammage P, Rorbach J, Vincent A, Rebar E, Minczuk M. Mitochondrially targeted ZFNs for selective degradation of pathogenic mitochondrial genomes bearing large-scale deletions or point mutations. EMBO Mol Med. 2014;6:458-66 pubmed publisher
Schneppenheim J, Hüttl S, Mentrup T, Lüllmann Rauch R, Rothaug M, Engelke M, et al. The intramembrane proteases signal Peptide peptidase-like 2a and 2b have distinct functions in vivo. Mol Cell Biol. 2014;34:1398-411 pubmed publisher
Fan X, Jin W, Lu J, Wang J, Wang Y. Rapid and reversible knockdown of endogenous proteins by peptide-directed lysosomal degradation. Nat Neurosci. 2014;17:471-80 pubmed publisher
Nandadasa S, Tao Q, Shoemaker A, Cha S, Wylie C. Regulation of classical cadherin membrane expression and F-actin assembly by alpha-catenins, during Xenopus embryogenesis. PLoS ONE. 2012;7:e38756 pubmed publisher
Kocer S, Wang H, Malbon C. "Shaping" of cell signaling via AKAP-tethered PDE4D: Probing with AKAR2-AKAP5 biosensor. J Mol Signal. 2012;7:4 pubmed publisher
Ariumi Y, Kuroki M, Dansako H, Abe K, Ikeda M, Wakita T, et al. The DNA damage sensors ataxia-telangiectasia mutated kinase and checkpoint kinase 2 are required for hepatitis C virus RNA replication. J Virol. 2008;82:9639-46 pubmed publisher
Xu C, Xu W, Palmer A, Reed J. BI-1 regulates endoplasmic reticulum Ca2+ homeostasis downstream of Bcl-2 family proteins. J Biol Chem. 2008;283:11477-84 pubmed publisher
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA