antibody

RPA32 Antibody

HY-P80975

10 μL;50 μL;100 μL

70;185;300 USD

monoclonal

domestic rabbit

nonconjugated

YA679

western blot, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

RPA32 Antibody

antibody

Virus

Monoclonal

Non-conjugated

YA679

10 μL;50 μL;100 μL

70;185;300 USD

Primary Antibodies

Human, Mouse, Rat

The related gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. The subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways.

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

WB, IHC-P, ICC/IF, IP

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.