antibody

Phospho-Chk2 Antibody

HY-P80799

10 μL;50 μL;100 μL

70;185;300 USD

polyclonal

domestic rabbit

nonconjugated

western blot, immunocytochemistry, immunohistochemistry - paraffin section

Phospho-Chk2 Antibody

antibody

Rabbit

Polyclonal Antibody

Unconjugated

10 μL;50 μL;100 μL

70;185;300 USD

Primary Antibodies

Human, Mouse, Rat

Chk2: In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

CHEK2; CDS1; CHK2; RAD53; Serine/threonine-protein kinase Chk2; CHK2 checkpoint homolog; Cds1 homolog; Hucds1; hCds1; Checkpoint kinase 2

Synthetic phosphopeptide corresponding to residues surrounding Thr68 of Human Chk2.AA range:35-84.

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

WB, ICC/IF, IHC-P

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.