protein

ISG15 E1/UBE1L Protein, Human (sf9, His, StrepⅡ)

HY-P79454

10 μg;50 μg

200;560 USD

ISG15 E1/UBE1L Protein, Human (sf9, His, StrepⅡ)

proteins

Sf9 insect cells

10 μg;50 μg

200;560 USD

The ISG15 E1/UBE1L protein initiates ubiquitination by adenylating the C-terminal glycine of ubiquitin with ATP to form a thioester bond with its cysteine residue. This produces ubiquitin-E1 thioester and free AMP. ISG15 E1/UBE1L Protein, Human (sf9, His, Strep) is the recombinant human-derived ISG15 E1/UBE1L protein, expressed by Sf9 insect cells , with N-8*His, N-Strep labeled tag. The total length of ISG15 E1/UBE1L Protein, Human (sf9, His, Strep) is 1011 a.a., .

Human

ISG15 E1/UBE1L protein initiates the ubiquitination process by activating ubiquitin through a two-step mechanism. It begins by adenylating the C-terminal glycine residue of ubiquitin with ATP and subsequently forms a thioester bond between this glycine residue and a cysteine residue in E1. This results in the generation of a ubiquitin-E1 thioester and free AMP. In addition to its role in ubiquitin activation, ISG15 E1/UBE1L catalyzes the ISGylation of the influenza A virus NS1 protein, highlighting its involvement in antiviral responses. The protein is actively engaged in protein modification processes, particularly protein ubiquitination, underscoring its significance in regulating cellular pathways through post-translational modifications.

Ubiquitin-like modifier-activating enzyme 7; UBA7; Ubiquitin-activating enzyme 7; D8; Ubiquitin-activating enzyme E1 homolog; UBE1L; UBE2

Greater than 95% as determined by reducing SDS-PAGE

Supplied as a 0.2 μm filtered solution of 50 mM HEPES (pH7.5), 200 mM NaCl, 1 mM DTT, 20% glycerol.

Shipping with dry ice