protein

UbcH7/UBE2L3 Protein, Human

HY-P79451

10 μg;50 μg;100 μg;500 μg

45;130;220;600 USD

UbcH7/UBE2L3 Protein, Human

proteins

E. coli

10 μg;50 μg;100 μg;500 μg

45;130;220;600 USD

UbcH7/UBE2L3 is a unique ubiquitin-conjugating E2 enzyme that cooperates with HECT-type and RBR family E3 ligases and lacks intrinsic E3-independent reactivity with lysine. It uniquely works with RBR family E3 enzymes such as PRKN, RNF31 and ARIH1, acting like a RING-HECT hybrid. UbcH7/UBE2L3 Protein, Human is the recombinant human-derived UbcH7/UBE2L3 protein, expressed by E. coli , with tag free.

Human

UbcH7/UBE2L3, a ubiquitin-conjugating enzyme E2, exhibits specificity in collaboration with HECT-type and RBR family E3 ubiquitin-protein ligases. Notably, its unique characteristic is the absence of intrinsic E3-independent reactivity with lysine, rendering it incompatible with most RING-containing E3 ubiquitin-protein ligases. However, it demonstrates activity with RBR family E3 enzymes such as PRKN, RNF31, and ARIH1, functioning akin to RING-HECT hybrids. Acting downstream of the E1 complex, UbcH7 catalyzes the covalent attachment of ubiquitin to target proteins and, in vitro, facilitates 'Lys-11'-linked polyubiquitination. Its involvement in the selective degradation of short-lived and aberrant proteins highlights its role in cellular quality control. Additionally, down-regulation during the S-phase suggests a contribution to cell cycle progression, while its impact on nuclear hormone receptors' transcriptional activity and potential role in myelopoiesis further underscore its multifaceted functions.

Ubiquitin-conjugating enzyme E2 L3; UBE2L3; E2 ubiquitin-conjugating enzyme L3; L-UBC; UbcH7; Ubiquitin carrier protein L3; Ubiquitin-conjugating enzyme E2-F1; Ubiquitin-protein ligase L3; UBCE7

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized from a 0.22 μm filtered solution of 20 mM Hepes, 50 mM Nacl, pH 7.4.

Room temperature in continental US; may vary elsewhere.