protein

Complement C3/C3a Protein, Human

HY-P7862

5 μg;10 μg;50 μg;100 μg

52;89;250;425 USD

Complement C3/C3a Protein, Human

proteins

E. coli

5 μg;10 μg;50 μg;100 μg

52;89;250;425 USD

Complement C3/C3a protein plays a key role in initiating the complement system through processing by C3 convertase in both the classical and alternative pathways. Complement C3/C3a protein is involved in various immune and inflammatory responses and also has a role in regulating blood pressure. Complement C3/C3a Protein, Human is a recombinant Complement C3/C3a protein expressed in E. coli, untagged, and consists of 77 amino acids (S672-R748) [1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11] .

Human

Complement C3 can be cleaved by C3 convertases or by blood proteases (such as thrombin) and immune cell-derived proteases (such as cathepsins) at sites of inflammation, producing two split products: Complement C3/C3a and Complement C3/C3b. Complement C3/C3a participates in various immune and inflammatory responses and exhibits bidirectional effects in different immune cells, potentially influencing immune responses and disease processes through different mechanisms [1] [2] . (a) In monocytes and macrophages, Complement C3/C3a mediates through TLR-4, promoting the production of pro-inflammatory mediators such as IL-1β, TNF-α, IL-6, and PGE2. (b) In mast cells, Complement C3/C3a induces histamine degranulation. (c) In eosinophils, it stimulates calcium mobilization, oxidative burst, and degranulation. (d) Additionally, Complement C3/C3a reduces neutrophil migration into the bloodstream by inhibiting mobilization factors. (e) Complement C3/C3a also plays a crucial role in brain inflammation by activating brain endothelial cells and recruiting leukocytes. (f) Complement C3/C3a can directly or indirectly alter T cell responses through interaction with receptors C3aR on antigen-presenting cells (APCs) and T cells [3] [4] [5] [6] . Complement C3/C3a is present in plasma-derived FVIII products and can promote T cell proliferation mediated by pdFVIII (FVIII products extracted from plasma) and lipopolysaccharides (HY-D1056) in dendritic cells (DCs) [7] . Additionally, Complement C3 is reported to have antioxidant stress activity, protecting human bronchial epithelial cells from cigarette smoke-induced oxidative stress and inhibiting apoptosis [7] .

rHuComplement C3/C3a; Complement Component C3a; C3a; Anaphylatoxin

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4 or PBS, pH 7.4, 8% trehalose.

Room temperature in continental US; may vary elsewhere.