protein

GM-CSF R alpha Protein, Human (Biotinylated, HEK293, His-Avi)

HY-P78110

20 μg;50 μg;100 μg

280;530;850 USD

GM-CSF R alpha Protein, Human (Biotinylated, HEK293, His-Avi)

proteins

HEK293

20 μg;50 μg;100 μg

280;530;850 USD

GM-CSF R alpha is the alpha subunit of the heterodimeric receptor for colony stimulating factor 2. GM-CSF R alpha binds to the cytokine with high specificity and low affinity [1] . GM-CSF R alpha binds to GM-CSF and induces cell differentiation [2] . GM-CSF R alpha monoclonal antibody decreases GM-CSFRα expression on GM-CSF-responsive cells and shows anti-inflammatory activity in rheumatoid arthritis (RA) [3] . GM-CSF R alpha Protein, Human (Biotinylated, HEK293, His-Avi) is a biotinylated recombinant protein with a C-Terminal His label and a C-Terminal Avi label, It consists of 298 amino acids (E23-G320) and is produced in HEK293 cells.

Human

GM-CSF R alpha is expressed on myeloid cells and on some non-hemopoietic cells, such as endothelial cells, not on T cells [2] . The amino acid sequence of human GM-CSF R alpha protein has low homology for mouse GM-CSF R alpha protein. GM-CSF receptor (GM-CSFR) consists of two subunits, an α-subunit, which binds the cytokine with low affinity, and a larger β-subunit (beta common; βc), responsible for signaling, forming a ternary receptor complex. Signal transduction in response to the cytokines interleukin (IL)-3 and IL-5 is also mediated by βc; therefore, receptor specificity is due to GM-CSFRα [1] . After binding GM-CSF to its receptor, Janus-kinase-2 (JAK-2) is recruited to the cytoplasmic domain of the β chain, and activation of JAK-2 occurs, which subsequently induces STAT-5 phosphorylation. This signaling pathway induces migration of STAT-5 dimers to the nucleus and promotes the transcription of various genes such as pim-1 and CIS to induce cell differentiation [2] . GM-CSFR α-subunit significantly increases positive synovial macrophages in the RA synovium. GM-CSFR α monoclonal antibody suppresses disease activity in the murine collagen-induced arthritis model [3] .

GM-CSF-R-alpha; GMCSFR-alpha; GMR-alpha; CSF2R; CSF2RY; CSF2RA; CDw116; CD116; CSF2RAX; CSF2RAY; CSF2RX; GMCSFR; GMR; SMDP4

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

Room temperature in continental US; may vary elsewhere.