protein

Cynomolgus IL-23 alpha & Mouse IL-12 beta Heterodimer Protein (HEK293, His)

HY-P77703

20 μg;50 μg

290;640 USD

Cynomolgus IL-23 alpha & Mouse IL-12 beta Heterodimer Protein (HEK293, His)

proteins

HEK293

20 μg;50 μg

290;640 USD

IL-23 alpha and IL-12 beta are the IL-23p19 and IL-12p40 subunits, respectively, composing IL-23 via heterodimerization manner. IL-23 binds to a heterodimeric receptor composed of IL-12RB1 and IL-23R, activates the Jak-Stat signaling cascade, acts on memory CD4(+) T cells preferentially [1] . IL-23 also involves in activation of several pathways including p38 MAPK or NF-κB and promotes the production of pro-inflammatory cytokines such as interleukin-17A/IL17A [2] . Cynomolgus IL-23 alpha & Mouse IL-12 beta Heterodimer Protein is produced in HEK293 cells, with total length of 481 amino acids and a N-Terminal His-tag.

Cynomolgus

IL-23 alpha and IL-12 beta, also known as IL23p19 and IL12p40, respectively, and composing IL-23 in a heterodimerization manner, exerts proinflammatory effects and promotes angiogenesis [1] [5] . IL-23 belongs to the IL-12 cytokine family together with IL-12 p35/p40, IL-27 EBI3/p28 and IL-35 EBI3/p35, and is produced by various immune cells such as dendritic cells and macrophages upon Toll-like receptor signaling in tissues [3] . IL-23 has a preference expression on memory CD4(+) T cells, and activates the Jak-Stat signaling cascade. IL-23 leads to IL-23 receptor phosphorylation and forms a docking site to trigger phosphorylation signal of STAT3 and STAT4 [1] . IL-23 is a key factor perpetuating Th17 cell activation and cytokine production by binding IL-23 receptor to produce Th17 cytokines such as IL17 A, IL-17 F and IL-22 [2] . IL-23 also acts function on natural killer cells, results interferon-γ secretion increasing and enhances antibody-dependent cellular cytotoxicity [4] . The sequence of amino acids in IL-23 alpha proteins of cynomolgus shows high similarity with human (97.88%) and is very different from mouse (74.6%) and rat (79.37%) IL-23 facilitates development of inflammation in numerous other models of immune pathology where IL-12 had previously been implicated, including models of arthritis,intestinal inflammation, and psoriasis [6] [7] [8] .

IL23 alpha; IL12 beta; IL23 alpha&IL12 beta

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

Room temperature in continental US; may vary elsewhere.