protein

CBFB Protein, Human (His)

HY-P76767

2 μg;10 μg;50 μg;100 μg;500 μg;1 mg

35;84;235;400;1120;1800 USD

CBFB Protein, Human (His)

proteins

E. coli

2 μg;10 μg;50 μg;100 μg;500 μg;1 mg

35;84;235;400;1120;1800 USD

CBFB forms a heterodimeric complex core binding factor (CBF) with RUNX family proteins, which enhances the DNA binding ability of RUNX members (such as RUNX1, RUNX2, and RUNX3). Heterodimers bind to specific sites in enhancers and promoters to regulate the transcription of target genes. CBFB Protein, Human (His) is the recombinant human-derived CBFB protein, expressed by E. coli , with N-6*His labeled tag. The total length of CBFB Protein, Human (His) is 181 a.a., with molecular weight of ~25 kDa.

Human

CBFB, a core-binding factor, forms the heterodimeric complex, CBF, with RUNX family proteins, including RUNX1, RUNX2, and RUNX3. RUNX members exert transcriptional modulation on their target genes by recognizing the core consensus binding sequence 5'-TGTGGT-3', or exceptionally, 5'-TGCGGT-3', within regulatory regions through their runt domain. CBFB serves as a non-DNA-binding regulatory subunit that enhances the sequence-specific DNA-binding capacity of RUNX allosterically. These heterodimers bind to the core sites of various enhancers and promoters, such as murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3, and GM-CSF promoters. CBF complexes play a role in the repression of the ZBTB7B transcription factor during cytotoxic (CD8+) T cell development, acting as a transcriptional silencer by binding to the RUNX-binding sequence within the ZBTB7B locus, thereby facilitating cytotoxic T cell differentiation. The interaction involves heterodimerization with RUNX1, RUNX2, and RUNX3, and CBFB also interacts with COPRS and forms a complex with PRMT5 and RUNX1.

Core-binding factor subunit beta; CBF-beta; PEA2-beta; PEBP2-beta

Greater than 90% as determined by reducing SDS-PAGE

Lyophilized from a 0.2 μm filtered solution of sterile 50 mM Tris, 300 mM NaCl, 5% trehalose, 5% mannitol and 0.01% Tween 80, pH 7.4.

Room temperature in continental US; may vary elsewhere.