protein

Calcineurin A/PPP3CA Protein, Human (sf9, His)

HY-P76759

50 μg

380 USD

Calcineurin A/PPP3CA Protein, Human (sf9, His)

proteins

Sf9 insect cells

50 μg

380 USD

The calcineurin A/PPP3CA protein is a calcium-dependent, calmodulin-stimulated phosphatase. PPP3CA inhibits MAP3K14/NIK signaling and SLC9A1 activity. PPP3CA is involved in processes such as kidney development, salivary enzyme secretion, hippocampal homosynaptic depotentiation, keratinocyte survival, bone formation, and T cell proliferation. Calcineurin A/PPP3CA Protein, Human (sf9, His) is the recombinant human-derived Calcineurin A/PPP3CA protein, expressed by Sf9 insect cells , with N-His labeled tag.

Human

Calcineurin A, also known as PPP3CA, is a calcium-dependent, calmodulin-stimulated protein phosphatase that plays a pivotal role in transducing intracellular Ca(2+)-mediated signals. Known substrates often contain a PxIxIT motif and/or an LxVP motif, highlighting the specificity of its phosphatase activity. In response to elevated Ca(2+) levels, Calcineurin A dephosphorylates and activates phosphatase SSH1, leading to cofilin dephosphorylation. Furthermore, upon increased Ca(2+) levels following mitochondrial depolarization, Calcineurin A dephosphorylates DNM1L, inducing its translocation to the mitochondrion. The protein also positively regulates CACNA1B/CAV2.2-mediated Ca(2+) release probability at hippocampal neuronal soma and synaptic terminals. Calcineurin A dephosphorylates and activates transcription factor NFATC1, thereby regulating NFAT-mediated transcription by promoting nuclear translocation. Its broad array of functions includes the dephosphorylation of heat shock protein HSPB1, transcription factor ELK1, DARPP32, CRTC2, TFEB, and KLHL3, suggesting a crucial role in various cellular processes such as kidney development, secretion of salivary enzymes, bone formation, immune responses, and regulation of ion transport. The multifaceted roles of Calcineurin A underscore its significance in diverse cellular functions and signal transduction pathways.

Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform; CALNA; CNA

Greater than 90% as determined by reducing SDS-PAGE

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 10% Glycerol. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Room temperature in continental US; may vary elsewhere.