protein

S6K1/RPS6KB1 Protein, Human (sf9, GST)

HY-P76588

50 μg

280 USD

S6K1/RPS6KB1 Protein, Human (sf9, GST)

proteins

Sf9 insect cells

50 μg

280 USD

S6K1/RPS6KB1 is a kinase downstream of mTOR that regulates cellular processes critical for proliferation, growth, and cell cycle. It phosphorylates EIF4B, RPS6 and EEF2K and regulates protein synthesis by inhibiting the pro-apoptotic function of BAD, thereby enhancing cell survival. S6K1/RPS6KB1 Protein, Human (sf9, GST) is the recombinant human-derived S6K1/RPS6KB1 protein, expressed by Sf9 insect cells , with N-GST labeled tag.

Human

S6K1/RPS6KB1, a serine/threonine-protein kinase, functions downstream of mTOR signaling in response to growth factors and nutrients, orchestrating cellular processes crucial for proliferation, cell growth, and cell cycle progression. Through its phosphorylation of EIF4B, RPS6, and EEF2K, it regulates protein synthesis, contributing to cell survival by suppressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex, and upon mitogenic stimulation, phosphorylation by mTORC1 leads to dissociation and activation. S6K1 also plays a pivotal role in translation initiation by phosphorylating substrates in the pre-initiation complex. Furthermore, it controls translation elongation by phosphorylating EEF2 kinase in response to IGF1. Additionally, S6K1 is involved in feedback regulation of mTORC2 and mTORC1, mediating cell survival, and modulating insulin resistance. Notably, it participates in the regulation of fatty acid uptake and interferon-gamma-induced translation inhibition through its phosphorylation of EPRS. These multifaceted roles highlight the significance of S6K1 in coordinating diverse cellular responses to environmental cues.

Ribosomal protein S6 kinase beta-1; S6K1; P70S6K1; p70 S6KA; RPS6KB1; STK14A

Greater than 90% as determined by reducing SDS-PAGE

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 10% Glycerol, 1 mM GSH. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Room temperature in continental US; may vary elsewhere.