protein

RIOK1 Protein, Human (sf9, His-GST)

HY-P76578

5 μg;10 μg;50 μg

105;178;500 USD

RIOK1 Protein, Human (sf9, His-GST)

proteins

Sf9 insect cells

5 μg;10 μg;50 μg

105;178;500 USD

The RIOK1 protein is critical in the final cytoplasmic maturation of the 40S ribosomal subunit, actively processing 18S-E pre-rRNA into mature 18S rRNA. RIOK1 is critical for NOB1 and PNO1 recycling of 40S late precursors, and its association with 40S late subunits suggests involvement in a translation-like checkpoint cycle. RIOK1 Protein, Human (sf9, His-GST) is the recombinant human-derived RIOK1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of RIOK1 Protein, Human (sf9, His-GST) is 568 a.a., with molecular weight of ~94 kDa.

Human

The RIOK1 Protein plays a crucial role in the final stages of cytoplasmic maturation of the 40S ribosomal subunit, actively participating in the processing of 18S-E pre-rRNA to mature 18S rRNA. Essential for the recycling of NOB1 and PNO1 from the late 40S precursor, RIOK1's association with the very late 40S subunit intermediate suggests involvement in a translation-like checkpoint cycle preceding its binding to the 60S ribosomal subunit. Despite its proposed protein kinase domain acting predominantly as an ATPase, the catalytic activity dynamically regulates its association with the 40S subunit. Beyond its role in ribosomal biogenesis, RIOK1 acts as an adapter protein, facilitating the recruitment of NCL/nucleolin to the PRMT5 complex for symmetrical methylation, demonstrating its multifaceted contributions to cellular processes.

Serine/threonine-protein kinase RIO1; RIO kinase 1; RIO1

Greater than 85% as determined by reducing SDS-PAGE

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.4.

Shipping with dry ice.