protein

PLK1 Protein, Human (sf9, His)

HY-P76550

5 μg;10 μg;50 μg;100 μg

55;90;250;400 USD

PLK1 Protein, Human (sf9, His)

proteins

Sf9 insect cells

5 μg;10 μg;50 μg;100 μg

55;90;250;400 USD

PLK1 is a serine/threonine protein kinase that centrally coordinates key functions during the M phase of the cell cycle. It regulates centrosome maturation, spindle assembly, cohesin removal, inactivates APC/C inhibitors, and controls mitotic exit and cytokinesis. PLK1 Protein, Human (sf9, His) is the recombinant human-derived PLK1 protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of PLK1 Protein, Human (sf9, His) is 603 a.a., with molecular weight of ~66 kDa.

Human

PLK1, a serine/threonine-protein kinase, plays a pivotal role in orchestrating various essential functions throughout the M phase of the cell cycle. It regulates centrosome maturation and spindle assembly, facilitates the removal of cohesins from chromosome arms, inactivates anaphase-promoting complex/cyclosome (APC/C) inhibitors, and governs mitotic exit and cytokinesis. Operating by binding and phosphorylating proteins that are already phosphorylated on specific motifs recognized by the POLO box domains, PLK1 phosphorylates an extensive array of substrates, including BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, PRC1, RACGAP1/CYK4, SGO1, STAG2/SA2, TEX14, TOPORS, p73/TP73, TPT1, WEE1, HNRNPU, and others. PLK1's crucial roles include promoting centrosome functions and bipolar spindle assembly through the phosphorylation of KIZ, NEDD1, and NINL. Additionally, it governs mitotic exit and cytokinesis by phosphorylating CEP55, ECT2, KIF20A/MKLP2, CENPU, PRC1, and RACGAP1. PLK1's involvement extends to kinetochore functions, sister chromatid cohesion, and the regulation of various checkpoint proteins, positioning it as a key player in the intricate network governing cell cycle progression.

Serine/threonine-protein kinase PLK1; PLK-1; STPK13

Greater than 90% as determined by reducing SDS-PAGE

Supplied as a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, pH 7.4, 0.5 mM EDTA, 0.5 mM EGTA, 0.5 mM PMSF, 25% glycerol.

Shipping with dry ice