protein

CHST15 Protein, Human (HEK293, His)

HY-P76258

5 μg;10 μg;50 μg

46;75;210 USD

CHST15 Protein, Human (HEK293, His)

proteins

HEK293

5 μg;10 μg;50 μg

46;75;210 USD

The CHST15 protein is a sulfotransferase that catalyzes the transfer of sulfate to GalNAc 4-sulfate in chondroitin sulfate A to form chondroitin sulfate E with GlcA-GalNAc(4,6-SO(4)) units. It also sulfates the unique non-reducing terminal sequence GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), similar to thrombomodulin chondroitin sulfate. CHST15 Protein, Human (HEK293, His) is the recombinant human-derived CHST15 protein, expressed by HEK293 , with N-His labeled tag.

Human

CHST15 protein functions as a sulfotransferase with a distinctive role in transferring sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue in chondroitin sulfate A, leading to the formation of chondroitin sulfate E containing GlcA-GalNAc(4,6-SO(4)) repeating units. Additionally, CHST15 transfers sulfate to a unique non-reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), resulting in a highly sulfated structure reminiscent of thrombomodulin chondroitin sulfate. Beyond its role in chondroitin sulfate biosynthesis, CHST15 may function as a B-cell receptor involved in BCR ligation-mediated early activation, mediating regulatory signals crucial to B-cell development and the potential regulation of B-cell-specific RAG expression. However, the precise implications of these results in vivo remain unclear. The multifaceted activities of CHST15 highlight its importance in the intricate molecular processes governing chondroitin sulfate modifications and B-cell regulatory pathways, though further research is needed to elucidate its in vivo functions comprehensively.

Carbohydrate sulfotransferase 15; hBRAG; GalNAc4S-6ST; BRAG

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Room temperature in continental US; may vary elsewhere.