protein

RUVBL1 Protein, Human (sf9, His)

HY-P76016

100 μg

938 USD

RUVBL1 Protein, Human (sf9, His)

proteins

Sf9 insect cells

100 μg

938 USD

The RUVBL1 protein has DNA-stimulated ATPase and DNA helicase activities and can hexamerize for ATP hydrolysis. It is a member of the NuA4 complex and contributes to histone acetylation to activate genes. RUVBL1 Protein, Human (sf9, His) is the recombinant human-derived RUVBL1 protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of RUVBL1 Protein, Human (sf9, His) is 456 a.a., with molecular weight of ~57 kDa.

Human

RUVBL1 protein possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity, with hexamerization considered crucial for ATP hydrolysis, and adjacent subunits in the ring-like structure contributing to the ATPase activity. As a component of the NuA4 histone acetyltransferase complex, RUVBL1 is involved in the transcriptional activation of select genes, primarily through the acetylation of nucleosomal histones H4 and H2A. This modification can alter nucleosome-DNA interactions and promote interaction with other proteins that positively regulate transcription. The NuA4 complex, including the ATPase and helicase activities, is, in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. The NuA4 complex may also play a direct role in DNA repair when recruited to sites of DNA damage. Furthermore, RUVBL1 is a component of a SWR1-like complex responsible for the removal of histone H2A.Z/H2AZ1 from the nucleosome and is proposed as a core component of the chromatin remodeling INO80 complex, which exhibits DNA- and nucleosome-activated ATPase activity and catalyzes ATP-dependent nucleosome sliding. RUVBL1 is essential for cell proliferation, plays a role in oncogenic transformation by MYC, and modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. Additionally, RUVBL1 may bind plasminogen at the cell surface, enhancing plasminogen activation.

RuvB-like 1; ECP-54; NMP 238; Pontin 52; TIP49a; TAP54-alpha; RUVBL1; INO80H

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 10% Glycerol. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Room temperature in continental US; may vary elsewhere.