protein

HEXB/Hexosaminidase B Protein, Mouse (HEK293, His)

HY-P75808

5 μg;10 μg;50 μg;100 μg

45;75;200;340 USD

HEXB/Hexosaminidase B Protein, Mouse (HEK293, His)

proteins

HEK293

5 μg;10 μg;50 μg;100 μg

45;75;200;340 USD

HEXB, or Hexosaminidase B protein, hydrolyzes N-acetyl-D-hexosamine in glycoconjugates. It selectively acts on neutral oligosaccharides, not degrading other substrates. HEXB does not degrade GM2 gangliosides; only isozyme A does. During fertilization, HEXB contributes to the zona block to polyspermy by inactivating the sperm galactosyltransferase-binding site. This mechanism prevents sperm binding to the zona pellucida. HEXB/Hexosaminidase B Protein, Mouse (HEK293, His) is the recombinant mouse-derived HEXB/Hexosaminidase B protein, expressed by HEK293 , with C-His labeled tag. The total length of HEXB/Hexosaminidase B Protein, Mouse (HEK293, His) is 505 a.a., with molecular weight of ~59.4 kDa.

Mouse

HEXB, or Hexosaminidase B protein, plays a crucial role in hydrolyzing the non-reducing end N-acetyl-D-hexosamine and/or sulfated N-acetyl-D-hexosamine found in glycoconjugates. This includes oligosaccharide moieties from proteins, neutral glycolipids, and certain mucopolysaccharides. Interestingly, isozyme B exhibits selective substrate specificity, efficiently hydrolyzing neutral oligosaccharides while not acting on other substrates. It is important to note that only isozyme A is responsible for the degradation of GM2 gangliosides in the presence of GM2A. Additionally, during fertilization, HEXB contributes to the zona block to polyspermy, being present in the cortical granules of non-activated oocytes. Upon oocyte activation, HEXB is exocytosed during the cortical reaction, effectively inactivating the sperm galactosyltransferase-binding site. This molecular mechanism accounts for the block in sperm binding to the zona pellucida, as reported in studies.

Beta-hexosaminidase subunit beta; HCC-7; HEXB

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Room temperature in continental US; may vary elsewhere.