protein

GBA/Glucosylceramidase Protein, Human (HEK293, His)

HY-P75786

5 μg;10 μg;50 μg

120;190;540 USD

GBA/Glucosylceramidase Protein, Human (HEK293, His)

proteins

HEK293

5 μg;10 μg;50 μg

120;190;540 USD

GBA (glucosylceramidase) is a lysosomal enzyme that degrades lipids and aids in cell membrane turnover. It hydrolyzes glucosylceramide to form ceramide and glucose. GBA/Glucosylceramidase Protein, Human (HEK293, His) is the recombinant human-derived GBA/Glucosylceramidase protein, expressed by HEK293 , with C-His labeled tag.

Human

GBA (Glucosylceramidase) is a crucial enzyme located within the lysosomal compartment, playing a central role in the degradation of complex lipids and cellular membrane turnover. It catalyzes the hydrolysis of glucosylceramides (GlcCers), such as beta-D-glucosyl-(1 1')-N-acylsphing-4-enine, into free ceramides and glucose. This enzymatic activity is essential for the PKC-activated salvage pathway of ceramide formation. Additionally, GBA participates in a transglucosylation reaction, transferring glucose from GlcCer to cholesterol, a process preferred with GlcCers containing mono-unsaturated fatty acids. Under specific conditions, GBA may catalyze the reverse reaction, transferring glucose from cholesteryl 3-beta-D-glucoside to ceramide. Furthermore, GBA can hydrolyze cholesteryl 3-beta-D-glucoside, yielding glucose and cholesterol. While GBA exhibits lower activity with galactosylceramides (GalCers), it can also transfer galactose between GalCers and cholesterol in vitro. In contrast, xylosylceramide (XylCer) is not a significant substrate for hydrolysis, but it serves as a good xylose donor for transxylosylation activity, forming cholesteryl 3-beta-D-xyloside.

Lysosomal acid glucosylceramidase; Alglucerase; Beta-GC; SGTase; GC; GLUC

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or PBS, pH 8.0, 8% trehalose.

Room temperature in continental US; may vary elsewhere.