protein

NS5 Protein, Dengue virus 2 (His)

HY-P75704

5 μg;10 μg

60;100 USD

NS5 Protein, Dengue virus 2 (His)

proteins

E. coli

5 μg;10 μg

60;100 USD

NS5 Protein is part of the flavivirus RNA replication complex (RC) composed of viral non-structural proteins and host-cell cofactors. NS5 is the largest flavivirus protein, the most conserved, which act as two domains, the RNA-dependent RNA polymerase (RdRp) and RNA methyltransferase enzyme (MTase). NS5 plays a fundamental role in flavivirus replication, viral RNA methylation, RNA polymerization, and host immune system evasion. NS5 Protein, Dengue virus 2 (His) is the recombinant Virus-derived NS5 protein, expressed by E. coli , with N-His labeled tag. The total length of NS5 Protein, Dengue virus 2 (His) is 296 a.a., with molecular weight of ~35.5 kDa.

Virus

Non-structural protein 5 (NS5) is part of the flavivirus RNA replication complex (RC) composed of viral non-structural proteins and host-cell cofactors. NS5 is the largest flavivirus protein, the most conserved, which act as two domains, the RNA-dependent RNA polymerase (RdRp) and RNA methyltransferase enzyme (MTase). The DENV-NS5 RdRp domain contributes to the viral replication stages, whereas the MTase initiates viral RNA capping and facilitates polyprotein translation. NS5 plays a fundamental role in viral RNA methylation, RNA polymerization, and host immune system evasion. It functions as a scaffold protein with different binding sites for the host STAT2 and ERC1 proteins, but sharing a requirement for UBR4. NS5 also interacts with hostcell proteins as hSTAT2 and contributes to the evasion of the type I interferon (IFN)-mediated innate immune response, which is the first step of host-cell defense against viral infections. NS5 is a potent antagonist of type I IFN signaling of the DENV proteins by suppressing the human JAK-STAT signaling [1] [2] [3] .

Dengue virus DENV-2 (strain New Guinea C) NS5 (methyltransferase domain) / Nonstructural protein 5 Protein; DENV-NS5 Protein; DENV

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 50 mM Tris-HCL, 300 mM NaCl, 200 mM arginine, pH 8.0.

Room temperature in continental US; may vary elsewhere.