protein

Aconitase 1/ACO1 Protein, Human (sf9, His)

HY-P75560

5 μg;10 μg;50 μg;100 μg

72;115;300;480 USD

Aconitase 1/ACO1 Protein, Human (sf9, His)

proteins

Sf9 insect cells

5 μg;10 μg;50 μg;100 μg

72;115;300;480 USD

The ACO1 protein is a bifunctional iron sensor that dynamically adapts to cellular iron levels. Under conditions of iron deficiency, it is converted to mRNA binding and regulates iron-related gene expression. Aconitase 1/ACO1 Protein, Human (sf9, His) is the recombinant human-derived Aconitase 1/ACO1 protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of Aconitase 1/ACO1 Protein, Human (sf9, His) is 889 a.a., with molecular weight of ~90 kDa.

Human

The Aconitase 1/ACO1 protein serves as a bifunctional iron sensor, dynamically adapting its activities based on cellular iron availability. Under conditions of iron deprivation, it undergoes a shift to mRNA binding activity, regulating the expression of genes involved in iron uptake, sequestration, and utilization. ACO1 binds to iron-responsive elements (IRES) in the untranslated regions of target mRNAs, thereby modulating translation—for instance, inhibiting the translation of ferritin and aminolevulinic acid synthase while stabilizing the mRNA of the transferrin receptor. Conversely, when cellular iron levels are elevated, ACO1 forms a 4Fe-4S cluster that precludes RNA binding activity and facilitates its aconitase activity. In this mode, ACO1 catalyzes the isomerization of citrate to isocitrate via cis-aconitate. This dual functionality underscores ACO1's crucial role in the intricate regulatory network governing cellular responses to varying iron concentrations.

Cytoplasmic aconitate hydratase; Aconitase; IRP1; IRE-BP 1; ACO1; IREB1

Greater than 90% as determined by reducing SDS-PAGE

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 300 mM NaCl, pH 8.0, 10% Glycerol.

Shipping with dry ice