protein

AKR1C2 Protein, Human (His)

HY-P75520

5 μg;10 μg;50 μg

94;160;450 USD

AKR1C2 Protein, Human (His)

proteins

E. coli

5 μg;10 μg;50 μg

94;160;450 USD

AKR1C2 protein: Cytoplasmic aldehyde-keto reductase, which uses NADH and NADPH to reduce ketosteroids to hydroxysteroids. AKR1C2 Protein, Human (His) is the recombinant human-derived AKR1C2 protein, expressed by E. coli , with N-6*His labeled tag. The total length of AKR1C2 Protein, Human (His) is 323 a.a., with molecular weight of ~38 kDa.

Human

AKR1C2 Protein is a cytosolic aldo-keto reductase that plays a pivotal role in catalyzing the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids. This enzyme exhibits a likely reductase function in vivo, as evidenced by the inhibition of its oxidase activity in vitro by physiological concentrations of NADPH. AKR1C2 displays a broad positional specificity, acting on positions 3, 17, and 20 of steroids, thereby regulating the metabolism of hormones such as estrogens and androgens. Collaborating with 5-alpha/5-beta-steroid reductases, it contributes to the conversion of steroid hormones into 3-alpha/5-alpha and 3-alpha/5-beta-tetrahydrosteroids. Furthermore, AKR1C2 catalyzes the inactivation of the potent androgen 5-alpha-dihydrotestosterone (5-alpha-DHT) to 5-alpha-androstane-3-alpha,17-beta-diol (3-alpha-diol). Specifically, it is capable of producing 17beta-hydroxy-5alpha-androstan-3-one/5alphaDHT and may also reduce conjugated steroids, such as 5alpha-dihydrotestosterone sulfate. Additionally, the protein exhibits an affinity for bile acids, further emphasizing its involvement in diverse metabolic pathways.

AKR-1C2; Aldo-keto reductase family 1 member C2; DD-2; AKR1C2

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

Room temperature in continental US; may vary elsewhere.