protein

DAP10 Protein, Human (HEK293, mFc)

HY-P75310

5 μg;10 μg;50 μg;100 μg;500 μg

45;70;200;340;950 USD

DAP10 Protein, Human (HEK293, mFc)

proteins

HEK293

5 μg;10 μg;50 μg;100 μg;500 μg

45;70;200;340;950 USD

DAP10 is a transmembrane adapter protein that forms the activating receptor KLRK1-HCST, which is critical for cytotoxicity in target cells expressing stress-inducing ligands. It is activated by ULBP, triggering calcium mobilization and activating PI3K, MAP2K/ERK and JAK2/STAT5 signaling pathways. DAP10 Protein, Human (HEK293, mFc) is the recombinant human-derived DAP10 protein, expressed by HEK293 , with C-mFc labeled tag. The total length of DAP10 Protein, Human (HEK293, mFc) is 30 a.a., with molecular weight of ~33-45 kDa.

Human

DAP10, a transmembrane adapter protein, forms an activation receptor, KLRK1-HCST, in lymphoid and myeloid cells, playing a pivotal role in triggering cytotoxicity against target cells expressing stress-induced ligands such as MHC class I chain-related MICA and MICB, as well as UL16-binding proteins (ULBPs). This receptor, activated by the interaction of ULBPs with KLRK1-HCST, initiates calcium mobilization and activates the PI3-kinase PIK3R1, MAP2K/ERK, and JAK2/STAT5 signaling pathways. The presence of both PIK3R1 and GRB2 is essential for full KLRK1-HCST-mediated activation, leading to the ultimate killing of target cells. In NK cells, KLRK1-HCST signaling induces cytotoxicity and enhances cytokine production, while in T-cells, it primarily provides costimulation for TCR-induced signals. The KLRK1-HCST receptor plays a crucial role in immune surveillance against tumors, and its absence results in the escape of melanoma cells from NK cell-mediated immune surveillance. DAP10 interacts with CLEC5A, forming a CLEC5A/TYROBP/HCST trimolecular complex, and it homodimerizes, with disulfide linkages. The heterohexamer comprises four subunits of HCST/DAP10 and two subunits of KLRK1, and it interacts with PIK3R1, GRB2, KLRK1, and CD300H.

Hematopoietic cell signal transducer; HCST; DAP10; KAP10; PIK3AP

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Room temperature in continental US; may vary elsewhere.