protein

FHIT Protein, Human (His)

HY-P75187

5 μg;10 μg;50 μg;100 μg

35;45;115;190 USD

FHIT Protein, Human (His)

proteins

E. coli

5 μg;10 μg;50 μg;100 μg

35;45;115;190 USD

FHIT protein has multiple enzymatic activities, including dinucleoside triphosphohydrolase, adenylate sulfatase and adenosine 5'-monophosphate amidase, and functions as a tumor suppressor. It regulates CTNNB1-mediated transcriptional activation, affecting key genes such as CCND1 and BIRC5. FHIT Protein, Human (His) is the recombinant human-derived FHIT protein, expressed by E. coli , with C-His labeled tag. The total length of FHIT Protein, Human (His) is 147 a.a., with molecular weight of ~18 kDa.

Human

FHIT protein exhibits dinucleoside triphosphate hydrolase activity, specifically cleaving P(1)-P(3)-bis(5'-adenosyl) triphosphate (Ap3A) to generate AMP and ADP. Additionally, it displays adenylylsulfatase activity, hydrolyzing adenosine 5'-phosphosulfate into AMP and sulfate, as well as adenosine 5'-monophosphoramidase activity, breaking down purine nucleotide phosphoramidates like adenosine 5'-monophosphoramidate (AMP-NH2) to produce AMP and NH2. Acting as a tumor suppressor, FHIT plays a role in modulating transcriptional activation by CTNNB1, influencing the expression of critical genes related to cell proliferation and survival, such as CCND1 and BIRC5. Furthermore, FHIT participates in apoptosis induction through SRC and AKT1 signaling pathways, inhibits MDM2-mediated proteasomal degradation of p53/TP53, and contributes to p53/TP53-mediated apoptosis. The apoptotic effect may involve FHIT's ability to bind Ap3A or related compounds, and it can also be attributed, in part, to its mitochondrial form, which enhances mitochondrial calcium uptake and sensitizes low-affinity Ca(2+) transporters.

Bis(5'-adenosyl)-triphosphatase; AP3A hydrolase; AP3Aase; FHIT

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 8.0, 10% Glycerol.

Room temperature in continental US; may vary elsewhere.