protein

HEXB/Hexosaminidase B Protein, Human (HEK293, His)

HY-P74890

5 μg;10 μg;50 μg

88;150;420 USD

HEXB/Hexosaminidase B Protein, Human (HEK293, His)

proteins

HEK293

5 μg;10 μg;50 μg

88;150;420 USD

HEXB (hexosaminidase B) proteolytically hydrolyzes N-acetyl-D-hexosamine and/or sulfated residues in glycoconjugates, including oligosaccharides, glycolipids, and mucopolysaccharides. B isoenzymes efficiently hydrolyze neutral oligosaccharides, making them unique. HEXB/Hexosaminidase B Protein, Human (HEK293, His) is the recombinant human-derived HEXB/Hexosaminidase B protein, expressed by HEK293 , with C-His labeled tag. The total length of HEXB/Hexosaminidase B Protein, Human (HEK293, His) is 514 a.a., with molecular weight of 60-66 kDa.

Human

HEXB (Hexosaminidase B) protein exhibits hydrolytic activity towards the non-reducing end N-acetyl-D-hexosamine and/or sulfated N-acetyl-D-hexosamine residues found in various glycoconjugates, including oligosaccharide moieties from proteins, neutral glycolipids, and specific mucopolysaccharides. Notably, the B isozyme demonstrates efficient hydrolysis of neutral oligosaccharides, distinguishing it from other substrates. While isozyme A is responsible for the degradation of GM2 gangliosides in the presence of GM2A, isozyme B does not participate in this process. During fertilization, HEXB, specifically isozyme B, plays a crucial role in the zona block to polyspermy. It is localized in the cortical granules of non-activated oocytes and is exocytosed during the cortical reaction triggered by oocyte activation. This exocytosis results in the inactivation of the sperm galactosyltransferase-binding site, contributing to the prevention of sperm binding to the zona pellucida and ensuring the integrity of the zona block to polyspermy.

Beta-hexosaminidase subunit beta; HCC-7; HEXB

Greater than 95% as determined by reducing SDS-PAGE.

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

Shipping with dry ice.