protein

ITK Protein, Mouse (sf9, His-GST)

HY-P74795

50 μg

350 USD

ITK Protein, Mouse (sf9, His-GST)

proteins

Sf9 insect cells

50 μg

350 USD

ITK protein is a tyrosine kinase that plays a crucial role in directing adaptive immune responses by controlling the development, function, and differentiation of conventional T cells and unconventional NKT cells.After T cell receptor (TCR) activation by antigen-presenting cells (APC), ITK is recruited to the cell membrane and phosphorylated by LCK, thereby making it fully activated.ITK Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived ITK protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

Mouse

ITK protein, a tyrosine kinase, assumes a critical role in orchestrating the adaptive immune response by governing the development, function, and differentiation of both conventional T-cells and nonconventional NKT-cells. Upon activation of the T-cell receptor (TCR) by antigen-presenting cells (APC), ITK is recruited to the cell membrane in close proximity to the stimulated TCR receptor, where it undergoes phosphorylation by LCK. This phosphorylation initiates ITK autophosphorylation, culminating in its full activation. Once activated, ITK phosphorylates PLCG1, activating this lipase and triggering the subsequent cleavage of its substrates. Consequently, calcium is released from the endoplasmic reticulum into the cytoplasm, facilitating the translocation of the nuclear activator of activated T-cells (NFAT) into the nucleus to execute its transcriptional functions. ITK also phosphorylates two essential adapter proteins, the linker for activation of T-cells/LAT protein and LCP2, leading to the recruitment of various signaling molecules, such as VAV1. This intricate signaling cascade ultimately results in lymphokine production, T-cell proliferation, and differentiation. Moreover, ITK is indispensable for TCR-mediated calcium response in gamma-delta T-cells, potentially influencing the transcriptomic signature in the Vgamma2-positive subset of immature gamma-delta T-cells. Additionally, ITK phosphorylates TBX21 at 'Tyr-525,' mediating its interaction with GATA3. The multifaceted functions of ITK underscore its pivotal role in shaping the adaptive immune response.

Tyrosine-protein kinase ITK/TSK; IL-2-inducible T-cell kinase; ITK; EMT; LYK

Greater than 90% as determined by reducing SDS-PAGE

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, pH 7.5, 10% Glycerol, 0.5 mM GSH. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Room temperature in continental US; may vary elsewhere.