protein

UBA1 Protein, Human (sf9, His-GST)

HY-P74485

5 μg;10 μg;50 μg;100 μg;500 μg;1 mg

50;80;200;320;1060;1680 USD

UBA1 Protein, Human (sf9, His-GST)

proteins

Sf9 insect cells

5 μg;10 μg;50 μg;100 μg;500 μg;1 mg

50;80;200;320;1060;1680 USD

The UBA1 protein initiates ubiquitin conjugation, marking proteins for degradation in the ubiquitin-proteasome system. UBA1 Protein, Human (sf9, His-GST) is the recombinant human-derived UBA1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

Human

The UBA1 protein plays a pivotal role in cellular processes as it catalyzes the initial step in ubiquitin conjugation, marking cellular proteins for degradation through the ubiquitin-proteasome system, as documented in several studies. This enzymatic activity involves the activation of ubiquitin by initially adenylating its C-terminal glycine residue with ATP. Subsequently, UBA1 links this activated ubiquitin to the side chain of a cysteine residue in E1, forming a ubiquitin-E1 thioester and releasing free AMP. The indispensable function of UBA1 extends to critical cellular processes, including the formation of radiation-induced foci, timely DNA repair, and the response to replication stress. Furthermore, UBA1 plays a role in promoting the recruitment of TP53BP1 and BRCA1 at DNA damage sites, emphasizing its significance in orchestrating DNA repair pathways and maintaining genomic integrity.

Ubiquitin-like modifier-activating enzyme 1; Protein A1S9; UBA1; A1S9T; UBE1

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, pH 7.4, 10% Glycerol, 0.5 mM GSH or PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Room temperature in continental US; may vary elsewhere.