protein

USP5 Protein, Human (sf9, His)

HY-P74479

20 μg;50 μg

160;340 USD

USP5 Protein, Human (sf9, His)

proteins

Sf9 insect cells

20 μg;50 μg

160;340 USD

USP5 protein selectively cleaves branched multiubiquitin polymers, favoring 'Lys-48'-linked polyubiquitin disassembly. It exhibits lower affinity for linear and 'Lys-63'-linked polyubiquitin. USP5 knockdown leads to p53/TP53 accumulation, enhancing p53/TP53 transcriptional activity, as unanchored polyubiquitin competes with ubiquitinated p53/TP53 for proteasomal recognition, unlike MDM2. USP5 Protein, Human (sf9, His) is the recombinant human-derived USP5 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of USP5 Protein, Human (sf9, His) is 835 a.a., with molecular weight of ~100 kDa.

Human

USP5, a deubiquitinating enzyme, demonstrates a marked preference for cleaving both linear and branched multiubiquitin polymers, with a particular affinity for branched structures. It is actively involved in the disassembly of unanchored 'Lys-48'-linked polyubiquitin chains. While USP5 binds linear and 'Lys-63'-linked polyubiquitin with a comparatively lower affinity, its knockdown results in the accumulation of p53/TP53 and an elevation in p53/TP53 transcriptional activity. This effect is attributed to the ability of the unanchored polyubiquitin accumulating in the absence of USP5 to compete with ubiquitinated p53/TP53, but not with MDM2, for recognition by the proteasome.

Ubiquitin carboxyl-terminal hydrolase 5; Isopeptidase T; USP5; ISOT

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Room temperature in continental US; may vary elsewhere.