protein

15 Lipoxygenase 2 Protein, Human (sf9)

HY-P74437

20 μg

300 USD

15 Lipoxygenase 2 Protein, Human (sf9)

proteins

Sf9 insect cells

20 μg

300 USD

15-lipoxygenase 2 (15-LOX2) is a nonheme iron-containing dioxygenase essential for the peroxidation of polyunsaturated fatty acids (PUFA). It exhibits peroxidase activity towards arachidonic acid and linoleic acid, producing bioactive lipid mediators. 15 Lipoxygenase 2 Protein, Human (sf9) is the recombinant human-derived 15 Lipoxygenase 2 protein, expressed by Sf9 insect cells , with tag free. and with residual Gly-Pro in N-terminal (not related to functional changes), , , , mutation. The total length of 15 Lipoxygenase 2 Protein, Human (sf9) is 676 a.a., with molecular weight of 62-66 kDa.

Human

15-Lipoxygenase 2 (15-LOX2) is a non-heme iron-containing dioxygenase with a crucial role in catalyzing stereo-specific peroxidation of both free and esterified polyunsaturated fatty acids (PUFAs), generating a diverse array of bioactive lipid mediators. This enzyme exhibits peroxidase activity on arachidonate, producing (15S)-hydroperoxyeicosatetraenoate/(15S)-HPETE, and on linoleate, generating 13-hydroperoxyoctadecadienoate/13-HPODE. Moreover, it oxygenates arachidonyl derivatives such as 2-arachidonoylglycerol (2-AG), leading to the production of 15-hydroxyeicosatetraenoyl glycerol (15-HETE-G), which acts as a peroxisome proliferator-activated receptor alpha agonist. 15-LOX2 efficiently class-switches pro-inflammatory mediators into anti-inflammatory intermediates and participates in the sequential oxidations of docosahexaenoate (DHA) to generate specialized pro-resolving mediators (SPMs) like resolvin D5. Besides its role in lipid metabolism, 15-LOX2 may influence cell cycle progression, cell proliferation, cytokine secretion by macrophages, and macrophage differentiation into proatherogenic foam cells. Importantly, it does not convert arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid/(15S)-HPETE, highlighting its substrate specificity.

Polyunsaturated fatty acid lipoxygenase ALOX15B; 15-LOX-2; ALOX15B

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% Glycerol, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Room temperature in continental US; may vary elsewhere.