protein

PDK1 Protein, Human (sf9, His)

HY-P73718

50 μg

450 USD

PDK1 Protein, Human (sf9, His)

proteins

Sf9 insect cells

50 μg

450 USD

PDK1 protein is a key kinase that controls glucose and fatty acid metabolism by phosphorylating pyruvate dehydrogenase subunits PDHA1 and PDHA2. This molecular switch inhibits pyruvate dehydrogenase, fine-tunes metabolite flux and downregulates aerobic respiration. PDK1 Protein, Human (sf9, His) is the recombinant human-derived PDK1 protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of PDK1 Protein, Human (sf9, His) is 408 a.a., with molecular weight of ~45 kDa.

Human

PDK1, a pivotal kinase, exerts significant control over glucose and fatty acid metabolism by phosphorylating the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This phosphorylation event acts as a molecular switch, inhibiting pyruvate dehydrogenase activity and finely tuning metabolite flux through the tricarboxylic acid cycle. Consequently, PDK1 down-regulates aerobic respiration and impedes the conversion of pyruvate to acetyl-coenzyme A. Beyond its metabolic functions, PDK1 emerges as a critical player in cellular responses to hypoxia, where it plays a key role in promoting cell proliferation under oxygen-deprived conditions. Moreover, PDK1 serves a protective role by shielding cells against apoptosis triggered by hypoxia and oxidative stress, showcasing its multifaceted contributions to cellular homeostasis and stress response pathways.

Pyruvate dehydrogenase kinase isoform 1; PDK1; PDHK1

Greater than 90% as determined by reducing SDS-PAGE.

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 8.5, 10% glycerol.

Shipping with dry ice.