protein

IL-27 Protein, Human (CHO, His)

HY-P73199

5 μg;10 μg;20 μg

130;220;380 USD

IL-27 Protein, Human (CHO, His)

proteins

CHO

5 μg;10 μg;20 μg

130;220;380 USD

IL-27 (Interleukin-27) is a heterodimeric cytokine of the IL-12 family, composed of two subunits, EBI3 (Epstein-Barr-virus-induced molecule 3) and p28 [1] . IL-27 enhances the development, proliferation, and cytotoxic activity of CD8 + T cells, thereby indirectly promoting anti-tumor immunity [ 2] . IL-27 Protein, Human (CHO, His) is a recombinant protein with a His label that consists of two subunits, EBI3 (IL27B) (229 amino acids (M1-K229)) and IL27A (214 amino acids (F29-P243)), is produced by CHO cells.

Human

IL-27 is mainly produced by cells of myeloid origin such as monocytes, macrophages, dendritic cells, and microglial cells, in response to stimuli acting through Toll-like receptors or TNF-R-family members [3] . The amino acid sequence of human IL-27 protein has low homology for mouse IL-27 protein. IL-27 acts through a heterodimer receptor consisting of IL-27Rα (WSX1) and gp130 chains, activates the JAK/STAT signaling pathway, which mainly involves STAT1 and STAT3 phosphorylation. STAT3 activation by IL-27 induces the expression of SOCS3, which inhibits further IL-27 signaling in a negative feedback loop, through inhibition of JAK activity [3] . IL-27 plays multiple roles in proinflammatory and anti-inflammatory immune responses. IL-27 enhances NF-κB/AP-1 activity and increases IL-12p40, TNF-α, and IL-6 production, increases TLR4 and TLR5 expression [5] . IL-27 exerts inhibitory effects upon hPMSC adherence and proliferation, and promotes migration of hPMSCs. Besides, IL-27 can upregulate PDL1 expression and enhance the regulatory effects of hPMSCs on Th1 and Th2 cell differentiations and IL-10 secretion from CD4+T cells [6] . IL-27 induces the proliferation and differentiation in hematopoietic stem cells [7] . IL-27 increases IL-10 levels and the expression of p-STAT1, p-STAT3 [8] . IL-27 promotes the development and differentiation of CD4+IL-10+ T cells [9] .

Interleukin-27 subunit beta; IL-27B; Ebi3; Interleukin-27 subunit alpha; IL-27A; p28

Greater than 87% as determined by reducing SDS-PAGE

Lyophilized from a 0.2 μm filtered solution of 20 mM MOPS,150 mM NaCl, 0.05% CHAPS, PH7.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Room temperature in continental US; may vary elsewhere.