protein

DNase I Protein, Human (HEK293, His)

HY-P72974

5 μg;10 μg;100 μg

89;151;718 USD

DNase I Protein, Human (HEK293, His)

proteins

HEK293

5 μg;10 μg;100 μg

89;151;718 USD

The DNase I protein is a serum endonuclease secreted by a variety of exocrine and endocrine organs and expressed in non-hematopoietic tissues. It preferentially selects protein-free DNA, plays a key role in apoptosis-induced cell death, and inhibits actin polymerization by specifically binding to G-actin. DNase I Protein, Human (HEK293, His) is the recombinant human-derived DNase I protein, expressed by HEK293 , with C-His labeled tag. The total length of DNase I Protein, Human (HEK293, His) is 282 a.a., with molecular weight of ~37 kDa.

Human

DNase I, a serum endonuclease, is secreted by a diverse array of exocrine and endocrine organs. It is expressed in non-hematopoietic tissues and exhibits a preference for cleaving protein-free DNA. Apart from its general role as an endonuclease, DNase I plays a crucial role in apoptosis-induced cell death. Additionally, it binds specifically to G-actin, hindering actin polymerization. In collaboration with DNASE1L3, DNase I is instrumental in the degradation of neutrophil extracellular traps (NETs), which primarily consist of DNA fibers and are released by neutrophils during inflammation. The degradation of intravascular NETs by DNase I and DNASE1L3 is essential to prevent the formation of clots that could obstruct blood vessels, thereby mitigating organ damage following inflammatory responses.

Deoxyribonuclease-1; DNase I; Dornase alfa; DNASE1; DNL1; DRNI

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Room temperature in continental US; may vary elsewhere.