protein

Melanoma antigen preferentially/PRAME, Human (His)

HY-P72258

2 μg;10 μg;50 μg

41;100;282 USD

Melanoma antigen preferentially/PRAME, Human (His)

proteins

E. coli

2 μg;10 μg;50 μg

41;100;282 USD

PRAME is located in the CRL2 E3 ubiquitin-protein ligase complex and directs ubiquitination and degradation, specifically targeting the truncated MSRB1/SEPX1 selenoprotein.It is recruited to active promoter regions and is involved in chromatin regulation.Melanoma antigen preferentially/PRAME, Human (His) is the recombinant human-derived Melanoma antigen preferentially/PRAME, expressed by E.coli , with N-6*His labeled tag.

Human

PRAME serves as the substrate-recognition component within the Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex, orchestrating the ubiquitination and subsequent degradation of target proteins. The CRL2(PRAME) complex specifically mediates the ubiquitination and degradation of truncated MSRB1/SEPX1 selenoproteins generated by failed UGA/Sec decoding. In the nucleus, this complex is recruited to epigenetically and transcriptionally active promoter regions bound by nuclear transcription factor Y (NFY), suggesting its involvement in chromatin regulation. PRAME functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB, and RARG. This repression activity prevents retinoic acid-induced cell proliferation arrest, differentiation, and apoptosis. The multifaceted role of PRAME highlights its significance in protein modification, particularly in the context of protein ubiquitination and cellular regulatory processes.

CT130; MAPE; OIP4; OPA interacting protein 4

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized from a 0.2 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

Room temperature in continental US; may vary elsewhere.