protein

DDX58 Protein, Human (His)

HY-P72170

5 μg;10 μg;20 μg;50 μg;100 μg

62;100;160;300;510 USD

DDX58 Protein, Human (His)

proteins

E. coli

5 μg;10 μg;20 μg;50 μg;100 μg

62;100;160;300;510 USD

The DDX58 protein is an innate immune receptor that detects cytoplasmic viral nucleic acids and initiates a signaling cascade for type I interferon production.It forms ribonucleoprotein complexes with viral RNA and promotes homo-oligomerization.DDX58 Protein, Human (His) is the recombinant human-derived DDX58 protein, expressed by E.coli , with N-6*His labeled tag.

Human

DDX58 protein serves as an innate immune receptor crucial for detecting cytoplasmic viral nucleic acids, initiating a downstream signaling cascade that leads to the production of type I interferons and pro-inflammatory cytokines. It forms a ribonucleoprotein complex with viral RNAs, promoting homooligomerization into filaments. This oligomerization facilitates the recruitment of RNF135, an E3 ubiquitin-protein ligase, which amplifies RIG-I-mediated antiviral signaling. Upon activation, DDX58 associates with MAVS/IPS1, activating TBK1 and IKBKE kinases, leading to the phosphorylation of IRF3 and IRF7. This activation prompts the transcription of antiviral immunological genes, including interferons IFN-alpha and IFN-beta. DDX58 recognizes various viral RNA ligands, such as 5'-triphosphorylated ssRNAs and dsRNAs, with the 5'-triphosphate moiety and blunt-end base pairing being crucial for its activity. The protein detects a broad range of RNA viruses and plays a role in antiviral signaling against dsDNA-containing viruses, such as Epstein-Barr virus. Additionally, it participates in granulocyte production, differentiation, bacterial phagocytosis, and the regulation of cell migration.

Antiviral innate immune response receptor RIG-I; DEAD box protein 58; RLR-1; RIG-I

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized from a 0.2 μm solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0 or PBS, 6% Trehalose, pH 7.4.

Room temperature in continental US; may vary elsewhere.