protein

CYP21A2 Protein, Human (His)

HY-P72164

5 μg;10 μg;50 μg

88;150;420 USD

CYP21A2 Protein, Human (His)

proteins

E. coli

5 μg;10 μg;50 μg

88;150;420 USD

CYP21A2 Protein, a cytochrome P450 monooxygenase, crucially hydroxylates progesterone and 17alpha-hydroxyprogesterone at C-21, yielding vital intermediates for mineralocorticoid and glucocorticoid biosynthesis in adrenal steroidogenesis. This enzymatic process involves molecular oxygen, with NADPH providing essential electrons via cytochrome P450 reductase (CPR). CYP21A2 plays a pivotal role in regulating adrenal cortex hormone production. CYP21A2 Protein, Human (His) is the recombinant human-derived CYP21A2 protein, expressed by E. coli , with N-6*His labeled tag. The total length of CYP21A2 Protein, Human (His) is 494 a.a., with molecular weight of ~59.9 kDa.

Human

The CYP21A2 protein, a cytochrome P450 monooxygenase, plays a pivotal role in adrenal steroidogenesis by catalyzing the hydroxylation at C-21 of progesterone and 17alpha-hydroxyprogesterone. This enzymatic activity leads to the formation of crucial intermediate metabolites, namely 11-deoxycorticosterone and 11-deoxycortisol, which are integral components in the biosynthetic pathway of mineralocorticoids and glucocorticoids. Mechanistically, CYP21A2 utilizes molecular oxygen, inserting one oxygen atom into the substrate and reducing the second into a water molecule. The necessary electrons for this process are provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). These biochemical transformations orchestrated by CYP21A2 are essential for the synthesis of key adrenal steroids, contributing significantly to the regulation of mineralocorticoid and glucocorticoid production in the adrenal cortex.

CYP21; 21 OHase; 21-OHase; CA21H ; CAH1; CP21A_HUMAN; CPS1; CYP21A 2; CYP21A2; CYP21B; Cytochrome P-450c21; Cytochrome P450 21; Cytochrome P450 C21B; Cytochrome P450 XXI; Cytochrome P450; family 21; subfamily A; polypeptide 2; Cytochrome P450; subfamily XXIA steroid 21 hydroxylase;

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized from a 0.2 μm solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

Room temperature in continental US; may vary elsewhere.