protein

PLY Protein, Streptococcus pneumoniae serotype 4 (N-His)

HY-P72057A

5 μg;10 μg;50 μg;100 μg

130;220;520;880 USD

PLY Protein, Streptococcus pneumoniae serotype 4 (N-His)

proteins

E. coli

5 μg;10 μg;50 μg;100 μg

130;220;520;880 USD

The PLY protein, a cholesterol-dependent toxin, induces cytolysis by forming pores in host membranes with a significant conformational change and oligomeric pore complex formation. Cholesterol is crucial for binding, insertion, and pore formation. PLY Protein, Streptococcus pneumoniae serotype 4 (N-His) is the recombinant PLY protein, expressed by E. coli , with N-His labeled tag. The total length of PLY Protein, Streptococcus pneumoniae serotype 4 (N-His) is 470 a.a., with molecular weight of ~55 kDa.

Others

The PLY protein operates as a cholesterol-dependent toxin, inducing cytolysis by forming pores in host membranes containing cholesterol. Following its binding to target membranes, the protein undergoes a significant conformational change, facilitating its insertion into the host membrane and the subsequent formation of an oligomeric pore complex. The presence of cholesterol is crucial for various stages of this process, including binding to host membranes, membrane insertion, and pore formation, with cholesterol binding mediated by a Thr-Leu pair in the C-terminus. Notably, PLY protein exhibits reversibility and can be inactivated by oxidation. The resulting homooligomeric pore complex, comprising 35 to 50 subunits, is formed upon insertion into the host membrane, contributing to the protein's cytolytic activity.

ply; SP_1923; Pneumolysin; Ply; Thiol-activated cytolysin

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80.

Room temperature in continental US; may vary elsewhere.