protein

CD40L/CD154/TRAP Protein, Human (HEK293, hFc-Flag)

HY-P72028

2 μg;5 μg;10 μg;50 μg;100 μg;250 μg;500 μg;1 mg

41;80;125;200;320;600;960;1540 USD

CD40L/CD154/TRAP Protein, Human (HEK293, hFc-Flag)

proteins

HEK293

2 μg;5 μg;10 μg;50 μg;100 μg;250 μg;500 μg;1 mg

41;80;125;200;320;600;960;1540 USD

CD40L (CD154; TRAP) is a ligand to CD40/TNFRSF5, acts function by generating a costimulatory signal that up-regulates IL-4 synthesis [1] . CD40L is specifically expressed on activated CD4+ T-lymphocytes and involves in activation of NF-κB/MAPK pathway [2] [3] . CD40L also involves in B cell differentiation, maturation, and apoptosis [4] . CD40L in human, cleaved into 2 chains of membrane form (1-261 a.a.) and soluble form (113-261 a.a.) which serves as a ligand for integrins (ITGA5:ITGB1 and ITGAV:ITGB3). CD40L/CD154/TRAP Protein, Human (HEK293, hFc-Flag) has a total length of 248 amino acids (M13-L261), is expressed in HEK392 cells with N-terminal hFc- and Flag-tag.

Human

CD40 Ligand (CD40L; CD154; TRAP) belongs to the tumor necrosis factor (TNF) family, is the ligand for CD40/TNFRSF5, specifically expressed on activated CD4+ T-lymphocytes [1] . CD40L is a type II transmembrane protein on B cells triggers important signals for B cell differentiation, maturation, and apoptosis [4] . CD40L acts function by cross-linking on T-cells to generate a costimulatory signal and thus enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation, as well as promoting the production of interferon-γ, and TNF-α [1] [4] . CD40L, binding with CD40 on antigen-presenting cells (APC), activates TNFR-associated factor 2- and IKK2-dependent pathways with stimulating I-κB kinase (IKK), increasing NF-κB DNA binding, and p65 nuclear translocation. The activation of I-κB kinase leads to strongly c-Jun N-terminal kinase activation as well as GST-I-κB and GST-p65 phosphorylation [2] . CD40L involves in MAPK pathways that strongly repress Bcl-6 with inducing the phosphorylation of Erk1/2, p38 and Jnk1/2 and activating IRF4 mediated by NF-κB [3] . CD40L also binds to and signals through several integrins, including αvβ3 and α5β1, which bind to the trimeric interface of CD40L. CD40L plays a major role in immune response and is a major target for inflammation [5] . CD40L is widely found in different animals, while the sequence in Human is highly similar to Rhesus macaque (98.08%), but very different from Rat and Mouse with similarities of 77.31% and 77.69%, respectively. CD40L in Human is cleaved into 2 chains of membrane form (1-261 a.a.) and soluble form (113-261 a.a.), while the soluble form derives from the membrane form by proteolytic processing. Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and a matrix metalloproteinases (MMP) inhibitor-sensitive pathway [6] .

CD40-L; T-cell antigen Gp39; NF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5; CD154; sCD40L;

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized from a 0.2 μm solution of PBS, 6% Trehalose, pH 7.4 or PBS, pH 7.4, 8% trehalose.

Room temperature in continental US; may vary elsewhere.