protein

Azoreductase/NQO1 Protein, Mouse (P.pastoris, His)

HY-P71831

5 μg;10 μg;50 μg

84;145;400 USD

Azoreductase/NQO1 Protein, Mouse (P.pastoris, His)

proteins

P. pastoris

5 μg;10 μg;50 μg

84;145;400 USD

The azoreductase/NQO1 protein reduces quinones to hydroquinone using NADH or NADPH as an electron donor.Azoreductase/NQO1 Protein, Mouse (P.pastoris, His) is the recombinant mouse-derived Azoreductase/NQO1 protein, expressed by P.pastoris , with N-6*His labeled tag.

Mouse

Azoreductase/NQO1 protein, a flavin-containing quinone reductase, plays a pivotal role in cellular redox regulation by catalyzing the two-electron reduction of quinones to hydroquinones, utilizing either NADH or NADPH as electron donors. Operating through a ping-pong kinetic mechanism, the enzyme sequentially transfers electrons from NAD(P)H to its flavin cofactor and then from the reduced flavin to the quinone, thereby bypassing the formation of semiquinone and reactive oxygen species. This process serves as a key component in quinone detoxification, regulating the cellular redox state. Azoreductase/NQO1 also contributes to the reduction of plasma membrane redox system components, such as coenzyme Q and vitamin quinones, generating antioxidant hydroquinone forms and potentially acting as a superoxide scavenger. Moreover, the protein exhibits versatility in its actions, as it can alternatively activate quinones and their derivatives, producing redox-reactive hydroquinones with DNA cross-linking antitumor potential. Furthermore, Azoreductase/NQO1 functions as a gatekeeper of the core 20S proteasome, interacting with tumor suppressors TP53 and TP73 in a NADH-dependent manner to inhibit their ubiquitin-independent degradation during oxidative stress.

Nqo1; Dia4; Nmo1; Nmor1; NAD(P)H dehydrogenase [quinone] 1; EC 1.6.5.2; Azoreductase; DT-diaphorase; DTD; Phylloquinone reductase; Quinone reductase 1; QR1

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized from a 0.2 μm sterile filtered 20 mM Tris-HCI, 0.5 M NaCI, 6% Trehalose, pH 8.0.

Room temperature in continental US; may vary elsewhere.